Results 11 to 20 of about 120,736 (335)

A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data [PDF]

open access: yesBioinformatics, 2014
MOTIVATION Open-source bacterial genome assembly remains inaccessible to many biologists because of its complexity. Few software solutions exist that are capable of automating all steps in the process of de novo genome assembly from Illumina data ...
D. Coil, G. Jospin, A. Darling
semanticscholar   +5 more sources

CD-HIT-OTU-MiSeq, an Improved Approach for Clustering and Analyzing Paired End MiSeq 16S rRNA Sequences [PDF]

open access: yes, 2017
In recent years, Illumina MiSeq sequencers replaced pyrosequencing platforms and became dominant in 16S rRNA sequencing. One unique feature of MiSeq technology, compared with Pyrosequencing, is the Paired End (PE) reads, with each read can be sequenced ...
Weizhong Li, Yuanyuan Chang
core   +2 more sources

Low-level point heteroplasmy detection in human mitogenomes amplified with different polymerases and sequenced on MiSeq FGx platform

open access: yesArchives of Forensic Medicine and Criminology, 2023
Introduction: Massively parallel sequencing of mitogenomes usually requires prior amplification.
Katarzyna Skonieczna   +3 more
doaj   +2 more sources

Comparison of MiSeq, MinION, and hybrid genome sequencing for analysis of Campylobacter jejuni [PDF]

open access: yesScientific Reports, 2021
The sequencing, assembly, and analysis of bacterial genomes is central to tracking and characterizing foodborne pathogens. The bulk of bacterial genome sequencing at the US Food and Drug Administration is performed using short-read Illumina MiSeq ...
Jason M. Neal-McKinney   +4 more
semanticscholar   +4 more sources

Hybrid De Novo Genome Assembly Using MiSeq and SOLiD Short Read Data. [PDF]

open access: yesPLoS ONE, 2015
A hybrid de novo assembly pipeline was constructed to utilize both MiSeq and SOLiD short read data in combination in the assembly. The short read data were converted to a standard format of the pipeline, and were supplied to the pipeline components such ...
Tsutomu Ikegami   +6 more
doaj   +2 more sources

Illumina MiSeq sequencing analysis of fungal diversity in stored dates [PDF]

open access: yesBMC Microbiology, 2017
Background Date palm has been a major fruit tree in the Middle East over thousands of years, especially in the Arabian Peninsula. Dates are consumed fresh (Rutab) or after partial drying and storage (Tamar) during off-season.
Ismail M. Al-Bulushi   +4 more
doaj   +3 more sources

Primer ID MiSeq Library Prep v2

open access: yes, 2017
This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID.
Shuntai Zhou
core   +2 more sources

Primer ID MiSeq Library Prep v1

open access: yes, 2017
This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID.
Shuntai Zhou
core   +2 more sources

Fungal Community Structure of Macroalga Ulva Intestinalis Revealed by Miseq Sequencing [PDF]

open access: yesSqualen, 2017
Since algae are well known as the host of fungi, the information about total fungal community associated with alga is needed to give the initial data that is useful for natural product discovery. The study on total fungal community associated with marine
Agusman Agusman, Feng Danqing
doaj   +3 more sources

Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform [PDF]

open access: yesNucleic Acids Research, 2015
With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs.
M. Schirmer   +5 more
semanticscholar   +5 more sources

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