The VHL tumor suppressor at the crossroad of protein folding, aggregation, and cancer. [PDF]
Mol OncolMutations, environmental stress, and chaperone dysfunction can destabilize pVHL, promoting its conversion from the native folded state into amyloid‐like assemblies. This transition may contribute to protein storage, cell dormancy, survival, and drug resistance.
Abad L, Tosatto SCE, Leonardi E.
europepmc +2 more sources
Sequential storage and release of microdroplets
Microsystems & Nanoengineering, 2021 Droplet microfluidic methods have opened up the possibility of studying a plethora of phenomena ranging from biological to physical or chemical processes at ultra low volumes and high throughput.
Zenon Toprakcioglu, Tuomas P. J. Knowles
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Shear-mediated sol-gel transition of regenerated silk allows the formation of Janus-like microgels
Scientific Reports, 2021 Microcapsules and microgels consisting of macromolecular networks have received increasing attention due to their biomedical and pharmaceutical applications.
Zenon Toprakcioglu, Tuomas P. J. Knowles
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Automated optimisation of solubility and conformational stability of antibodies and proteins
Nature Communications, 2023 Antibodies find key applications in research, diagnostics, and therapeutics, but their development can be impeded by poor stability or solubility. Here the authors developed a computational strategy that enables antibody optimisation, without affecting ...
Angelo Rosace +7 more
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Recent High-Resolution Structures of Amyloids Involved in Neurodegenerative Diseases
Frontiers in Aging Neuroscience, 2021 Amyloids are highly ordered aggregates composed of proteins or peptides. They are involved in several pathologies, including hallmark neurodegenerative disorders such as Alzheimer’s (AD) and Parkinson’s (PD).
Rodrigo Diaz-Espinoza
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Spatially non-uniform condensates emerge from dynamically arrested phase separation
Nature Communications, 2023 Biomolecular condensates with internal structure allow cells to further organise their processes. In this work the authors investigate how condensates can obtain an internal structure with droplets of dilute phase inside via kinetic, rather than purely ...
Nadia A. Erkamp +9 more
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Synthetic biology tools to promote the folding and function of RNA aptamers in mammalian cells
RNA Biology, 2023 RNA aptamers are structured RNAs that can bind to diverse ligands, including proteins, metabolites, and other small molecules. RNA aptamers are widely used as in vitro affinity reagents.
Qian Hou, Samie R. Jaffrey
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The Transthyretin/Oleuropein Aglycone Complex: A New Tool against TTR Amyloidosis
Pharmaceuticals, 2022 The release of monomers from the homotetrameric protein transthyretin (TTR) is the first event of a cascade, eventually leading to sporadic or familial TTR amyloidoses.
Francesco Bemporad +4 more
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A Role of Cholesterol in Modulating the Binding of α-Synuclein to Synaptic-Like Vesicles
Frontiers in Neuroscience, 2020 α-Synuclein (αS) is a presynaptic protein whose aggregation is associated with Parkinson’s disease (PD). Although the physiological function of αS is still unclear, several lines of evidence indicate that this protein may play a role in the trafficking ...
Wing K. Man +5 more
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Structural Specificity of Polymorphic Forms of α-Synuclein Amyloid
Biomedicines, 2023 The structural transformation producing amyloids is a phenomenon that sheds new light on the protein folding problem. The analysis of the polymorphic structures of the α-synuclein amyloid available in the PDB database allows analysis of the amyloid ...
Irena Roterman +2 more
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