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Longevity and mitochondrial membrane potential

Biochemistry (Moscow), 2012
In Saccharomyces cerevisiae yeast cells a decrease in the mitochondrial membrane potential caused by protonophores or by a loss of mitochondrial DNA leads to an increase in longevity (replicative life span). The loss of mitochondrial DNA also activates retrograde signaling that results in certain changes in transcription. Recently, Miceli and coauthors
D A, Knorre, F F, Severin
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Biogenesis of mitochondrial membrane proteins

Current Opinion in Cell Biology, 2009
Mitochondria are ubiquitous, double-membrane bound organelles, which have developed from endosymbiotic alpha-proteobacteria during evolution. Outer and inner membranes of mitochondria are equipped with characteristic sets of membrane proteins required for energy conversion, metabolite and protein transport, membrane fusion and fission, and signal ...
Becker, Thomas   +3 more
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Mitochondrial Membrane Potential Assay

2022
Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in various types of cells, such as HepG2 ...
Srilatha, Sakamuru   +3 more
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Assembling the mitochondrial outer membrane

Nature Structural & Molecular Biology, 2004
The general preprotein translocase of the outer mitochondrial membrane (TOM complex) transports virtually all mitochondrial precursor proteins, but cannot assemble outer-membrane precursors into functional complexes. A recently discovered sorting and assembly machinery (SAM complex) is essential for integration and assembly of outer-membrane proteins ...
Pfanner, Nikolaus   +3 more
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The arrangement of mitochondrial membranes and a new structural feature of the inner mitochondrial membranes

Journal of Ultrastructure Research, 1977
Rat heart muscle mitochondria were analyzed by means of high-resolution electron microscopy of ultrathin sections of tissue prepared according to the techniques of Sjostrand and Barajas [J. Ultrastruct. Res. 25, 121 (1968)] . These techniques aim at reducing the extent of changes in protein conformation as compared to conventional preparatory ...
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Electrophysiology of the inner mitochondrial membrane

Journal of Bioenergetics and Biomembranes, 1994
The application of electrophysiological techniques to mitochondrial membranes has allowed the observation and partial characterization of several ion channels, including an ATP-sensitive K(+)-selective one, a high-conductance "megachannel", a 107 pS anionic channel and three others studied at alkaline pH's.
Zoratti M, SZABO', ILDIKO'
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Lipid metabolism in mitochondrial membranes

Journal of Inherited Metabolic Disease, 2014
AbstractMitochondrial membranes have a unique lipid composition necessary for proper shape and function of the organelle. Mitochondrial lipid metabolism involves biosynthesis of the phospholipids phosphatidylethanolamine, cardiolipin and phosphatidylglycerol, the latter is a precursor of the late endosomal lipid bis(monoacylglycero)phosphate.
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Reaction of malonaldehyde with mitochondrial membranes

Mechanisms of Ageing and Development, 1982
Malonaldehyde formed by lipid oxidation is regarded as a main crosslinker in the formation of natural age pigment. To elucidate the mechanism of pigment formation the reaction of malonaldehyde with biomembranes using fluorescence spectroscopy has been studied. Rat liver mitochondrial ghosts or bovine serum albumin were reacted with malonaldehyde.
W X, Balcavage, T K, Alvager
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Membrane structure: Binary membranes of mitochondrial cristae

Experimental Cell Research, 1969
Abstract Sections of mitochondrial cristae membranes show a five-layered structure consisting of two outer and one central stained region with unstained layers between. Membranous fragments obtained by detergent fractionation of the cristae show three layers with stained layers on each side of a central unstained layer.
K S, Prezbindowski   +3 more
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Incorporation of β-sitosterol into mitochondrial membrane enhances mitochondrial function by promoting inner mitochondrial membrane fluidity

Journal of Bioenergetics and Biomembranes, 2012
Recent findings suggest that mitochondrial membrane fluidity could influence mitochondrial energy metabolism. β-sitosterol (BS) is a common plant sterol that is prevalent in plant oils, nuts, cereals and plant food products. Its chemical structure is very similar to that of cholesterol.
Chun, Shi, Fengming, Wu, Jie, Xu
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