Results 211 to 220 of about 491,817 (253)

Mitochondrial Respiration and Oxygen Tension

2017
Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with ...
Daniel S, Shaw, Karlia, Meitha, Michael J, Considine, Christine H, Foyer   +3 more
openaire   +2 more sources

ROLE OF PHOSPHOLIPIDS IN MITOCHONDRIAL RESPIRATION

Canadian Journal of Biochemistry and Physiology, 1959
The addition of heated snake venom solutions to suspensions of rat liver, kidney, or brain mitochondria results in an initial stimulation of the rate of respiration, which is approximately the same for a variety of substrates, followed by a rapid decline.The presence of phospholipase A in heated snake venom is demonstrated by the formation of ...
E, PETRUSHKA, J H, QUASTEL, P G, SCHOLEFIELD   +2 more
openaire   +2 more sources

The effect of camphor on mitochondrial respiration

Experientia, 1982
Camphor at less than 8 mumoles/mg protein reduced the rate of oxygen consumption by rat liver mitochondria. The effect occurs only with NAD+-linked substrates. Succinate linked respiration was inhibited but this appears to be caused by some conversion of succinate to malate.
D F, Guilland-Cumming, G J, Smith
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Lactate as a fuel for mitochondrial respiration

Acta Physiologica Scandinavica, 2000
Lactate production in skeletal muscle has now been studied for nearly two centuries and still its production and functional role at rest and during muscle contraction is a subject of debate. Historically, skeletal muscle was seen mainly as the site of lactate production during contraction and lactate production associated with a lack of muscle ...
openaire   +2 more sources

Uncoupling of mitochondrial respiration by ADP

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1977
Even when oxidative phosphorylation is blocked completely by addition of high concentrations of oligomycin plus aurovertin, the addition of ADP to a suspension of mitochondria containing a high concentration of ATP inside the mitochondria induces a stimulation of respiration and oxidation of nicotinamide nucleotide.
T A, Out, K, Krab, A, Kemp, E C, Slater
openaire   +2 more sources

Effect of peritonitis on mitochondrial respiration

Journal of Surgical Research, 1971
Abstract Rats were exposed to a lethal form of Klebsiella pneumoniae peritonitis and the mitochondrial respiration of their livers was studied either 18 hours after the injection of the organism or immediately after clinical death. No indication of impaired mitochondrial function was found compared to normal controls. The level of high-energy adenine
G A, Decker, A M, Daniel, S, Blevings, L D, Maclean   +3 more
openaire   +2 more sources

Cellular Control of Mitochondrial Respiration

1976
Oxygen delivery to tissues is essential for maintaining the oxygen tension at a level adequate to sustain the cellular energy supply via oxidative phosphorylation. The question is then how the cell regulates oxidative phosphorylation and, through this, the rate of oxygen consumption.
D F, Wilson, M, Erecińska, M, Stubbs, J G, Lindsay, C S, Owen   +4 more
openaire   +2 more sources

Control of Mitochondrial Respiration in the Heart In Vivo

Annual Review of Physiology, 1989
The role of the hydrolysis products of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and inorganic phosphate (Pi), in the control of myocardial respiration was evaluated in vivo using 31P NMR. These studies were conducted to evaluate whether increases in the ATP hydrolysis products can be detected through the cardiac cycle or during ...
F W, Heineman, R S, Balaban
openaire   +3 more sources

Effects of ferric nucleotides on mitochondrial respiration

Archives of Biochemistry and Biophysics, 1964
Abstract Addition of ferric adenosine diphosphates to rat liver mitochondria induced a burst of respiration similar to that given by ADP. The ratio of moles of added Fe(III)ADP to gram-atoms of oxygen removed during this burst was 20% greater than in the corresponding case with ADP.
E H, STRICKLAND, C R, GOUCHER
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Mitochondrial respiration measurement

Protocol Exchange, 2007
1. Isolate mitochondria by gently crushing 40 to 80 flies in a 10 ml Kontes homogenizer with 7 strokes of the pestle in 3 ml homogenization buffer consisting of 225 mM mannitol, 75 mM sucrose, 10 mM MOPS, 1 mM EGTA, 0.5% BSA, pH 7.2 at 4°C 38. 2. Filter the extracts through 8 layers of cheese cloth, and then centrifuge at 300 x G for 3 min in a Beckman
James Jiayuan Tong, Douglas C. Wallace
openaire   +1 more source