Results 91 to 100 of about 6,637 (223)

Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrity

New Phytologist, Volume 251, Issue 3, Page 1283-1293, August 2026.
Polyphenol oxidases (PPOs) depletion preserves endogenous proteins at their predicted molecular weights and enhances detectable enzymatic activities. (a) Less RbcL crosslinking in ppo mutant of Nicotiana benthamiana. Leaf extracts were incubated for 1 h, separated on protein gels, stained with Coomassie or analyzed by Western blot with the anti‐RbcL ...
Kaijie Zheng, Farnusch Kaschani, Emma C. Watts, Markus Kaiser, Renier A. L. van der Hoorn   +4 more
wiley   +1 more source

Mutasynthesis and Antibiotic Activity of Mupirocin Analogues

ChemBioChem, Volume 27, Issue 13, 14 July 2026.
A mutasynthetic strategy was developed to prepare pseudomonic acid analogues, and their antimicrobial activities were evaluated against five bacterial strains. Feeding studies with mutant strains of Pseudomonas fluorescens revealed that C7‐desepoxy‐PA‐B was metabolised to C7‐PA‐C with loss of the 8‐hydroxyl group, but no fatty acid chain extension ...
Sarah M. Husain, Luoyi Wang, Li‐Chen Han, James I. Bowen, Zhongshu Song, Felix de Courcy‐Ireland, Ashley J. Winter, Thomas J. Simpson, Paul R. Race, James Spencer, Matthew P. Crump, Christine L. Willis   +11 more
wiley   +1 more source

A Novel Plasma Heme Assay Reveals Disease Severity in Beta‐Thalassemia and Sickle Cell Anemia

American Journal of Hematology, Volume 101, Issue 7, Page 1706-1716, July 2026.
ABSTRACT Anemia results from imbalanced hemoglobin or red blood cell production and clearance. Hemolytic anemia, caused by premature red blood cell removal, can be intravascular (in blood) or extravascular (erythrophagocytosis). Hemolysis is common in Sickle Cell Disease (SCD) and Beta‐Thalassemia anemia (β‐thalassemia), the most prevalent inherited ...
Laurent Kiger, Nicolas Hebert, Laura Bencheikh, Vincent Malcor Deydier de Pierrefeu, Anne‐Laure Pham Hung d’Alexandry d’Orengiani, Sandia Adypagavane, Meghan Perkins, Laura Matabishi‐Bibi, France Pirenne, Claire Berquet, Stephane Moutereau, Serge Pissard, Frédéric Galactéros, Michael C. Marden, Pablo Bartolucci   +14 more
wiley   +1 more source

Oxidative drug metabolising enzymes in North Sea dab (Limanda limanda)

, 1997
In the framework of monitoring programmes organizedunder several sea protection conventions (HELSINKI Conv.,OSPAR Conv.) the contracting parties are requested todevelop appropriate techniques for Biological-Effect-Monitoring.
Kellermann, Hans-Jürgen, Vobach, Michael   +1 more
core  

Systematic review and scoring‐based selection of pharmacokinetic models for precision dosing of vancomycin in neonates and children

British Journal of Clinical Pharmacology, Volume 92, Issue 7, Page 2028-2048, July 2026.
This systematic review identified 112 population pharmacokinetic models of vancomycin in neonates and children. To support rational model selection for model‐informed precision, we developed and applied expert‐driven, transparent criteria that integrate both methodological rigour and clinical relevance.
Zoë Vander Elst, Pieter‐Jan Spiessens, Dorian Vanneste, Nada Dia, Matthias Gijsen, Isabel Spriet, Karel Allegaert, Erwin Dreesen, Anne Smits   +8 more
wiley   +1 more source

SPORCalc: A development of a database analysis that provides putative metabolic enzyme reactions for ligand-based drug design.

, 2009
Understanding both the enzyme reactions that contribute to intermediate metabolism and the biochemical fate of candidate therapeutic and toxic agents are essential for drug design.
Stein, Viktor, Smith, James
core  

Thermodynamic Evaluation of Dual Substrate Growth

Biotechnology and Bioengineering, Volume 123, Issue 7, Page 1825-1835, July 2026.
ABSTRACT Various C₁–C₂ compounds are increasingly available through electrochemical reduction of CO2. Although not always suitable as a sole substrate, these compounds can supplement a primary substrate like glucose to enhance microbial growth. Yet, the mechanisms underlying the effects of dual substrate consumption on growth rate and growth yield ...
Marit A. Verheijen, Tim Meyboom, Mark C. M. van Loosdrecht, Rebeca Gonzalez‐Cabaleiro   +3 more
wiley   +1 more source

Contribution to the elucidation of the mechanism of hepatic porphyria induced by hexachlorobenzene and related polyhalogenated hydrocarbons

, 1981
Polyhalogenated aromatic hydrocarbons are known to cause hepatic porphyria in man and in various species of animals. This disorder in porphyrin metabolism is attributed to a defect in the activity of the intermediary enzyme uroporphyrinogen decarboxylase
Debets, F.M.H.
core  

Identification of PRKCB, NLRC4, and TNFSF10 as Key Regulators of the Lipid Metabolism‐Autophagy Network in Atherosclerosis

Cell Biochemistry and Function, Volume 44, Issue 7, July 2026.
ABSTRACT Background The occurrence and development of atherosclerosis (AS) are closely related to disorders of lipid metabolism and dysfunction of autophagy. However, the key regulatory genes and immune microenvironment characteristics require further exploration. Methods This study integrated the bulk transcriptome and single‐cell RNA sequencing data,
Min Li, Yanjun Sun, Yanyi Sun, Zhenyue Chen   +3 more
wiley   +1 more source

Mechanistic Elucidation of BBOX-Catalyzed Hydroxylation and THP-Induced Oxidative Rearrangement via QM/MM Calculations. [PDF]

Molecules
Ruan Z, Li H, Liu Y, Zhang X, Li X.
europepmc   +1 more source