Results 121 to 130 of about 2,644,529 (258)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

On bipartite-mixed graphs

Mixed graphs can be seen as digraphs that have both arcs and edges (or digons, that is, two opposite arcs). In this paper, we consider the case where such graphs are bipartite.
Dalfó Simó, Cristina, Fiol Mora, Miquel Àngel, López Lorenzo, Nacho   +2 more
core  

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

Metric, edge-metric, mixed-metric, and fault-tolerant metric dimensions of geometric networks with potential applications

Scientific Reports
Resolvability parameters of graphs are widely applicable in fields like computer science, chemistry, and geography. Many of these parameters, such as the metric dimension, are computationally hard to determine. This paper focuses on Möbius-type geometric
Sakander Hayat, Rishi Naeem, Hafiz Muhammad Afzal Siddiqui, Amina Riaz, Muhammad Imran, Melaku Berhe Belay, Zeineb Klai   +6 more
doaj   +1 more source

DTS-MixNet: Dynamic Spatiotemporal Graph Mixed Network for Anomaly Detection in Multivariate Time Series

Big Data and Cognitive Computing
Anomaly detection in multivariate time series (MTS) remains challenging due to the presence of complex and dynamic spatiotemporal dependencies. To address this, we propose the Dynamic Spatiotemporal Graph Mixed Network (DTS-MixNet), which takes a sliding
Chengxun Tan, Jiayi Hu, Jian Li, Minmin Miao, Wenjun Hu, Shitong Wang   +5 more
doaj   +1 more source

Chromatic number of sparse colored mixed planar graphs

, 2009
International audienceA colored mixed graph has vertices linked by both colored arcs and colored edges. The chromatic number of such a graph G is defined as the smallest order of a colored mixed graph H such that there exists a (arc-color preserving ...
Alexandre Pinlou, André Raspaud, Amanda Montejano, Éric Sopena, Sopena, Eric, Raspaud, André, Pinlou, Alexandre, Montejano, Amanda   +7 more
core   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Beta1 subunits enable fentanyl‐induced BK channel activation and opioid regulation of cerebral artery diameter

FEBS Open Bio, EarlyView.
During the current opioid crisis, the majority of overdose deaths are attributed to ultra‐potent synthetic opioids, such as fentanyl. While fentanyl exerts its action via opioid receptors, we propose that potassium channels in the brain blood vessels may also sense fentanyl.
Shiwani Thapa, Mohammed Nooh, Akhil Geddati, Alex Dopico, Anna N. Bukiya   +4 more
wiley   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source