Results 191 to 200 of about 2,900,458 (300)

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

FEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa, Saori Kagoshima, Akira Yamada   +2 more
wiley   +1 more source

An Indonesian Study of Mixed Methods An Example of Methodological Triangulation

A mixed methods research study is where the researcher/s combines elements of qualitative and quantitative research approaches by converging different data sources.
Rusdi, Muhammad., Ahamad, Mohd Sanusi S., Roosli, Ruhizal.   +2 more
core  

Barriers and facilitators to telerehabilitation implementation: a mixed-methods study of German physiotherapists. [PDF]

BMC Health Serv Res
Stahn M, Roemhild M, Kopkow C, Rausch AK.   +3 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Implementation of Emotional Connection Training in Pediatric Primary Care: Mixed Methods Study. [PDF]

JMIR Med Educ
Erickson E, Amati JB, Dumitriu D, Miller-Fitzwater A, Best DL, Preiss D, Uejo E, Brown A, Crotty JE, Gillen L, Grier C, Gustafson KK, Shearman N, Lavallée A, Warmingham J, Arduin E, Shearman N, Young J, Sperling J.   +18 more
europepmc   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

A mixed methods evaluation of mechanisms for facilitation in pediatric primary care. [PDF]

Implement Sci Commun
Boggs JM, Williams N, Waller CR, Pandey M, Johnson C, Ahmedani BK, Pappas C, Bedjeti K, Maye M, Wolk CB, Buttenheim AM, Linn KA, Wright L, Daley MF, Small DS, Beidas RS.   +15 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Determinants of antimicrobial resistance practices in Ethiopia: A One Health mixed-methods study. [PDF]

One Health
Assefa GM, Wgebriel A, Tarekegn M, Gebreselassie G, Shifarraw S, Solomon A, Mihret M, Girmay AH, Belete GA, Abrie W, Majiwa F, Negash K.   +11 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source