Results 71 to 80 of about 5,584,216 (285)

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Mixed methods research in pedagogy: Characteristics, advantages and difficulties in application [PDF]

Zbornik Instituta za pedagoška istraživanja, 2015
The mixed methods research, as a new type of research, is discussed in the context of re-examining the relation between the two approaches, i.e. the possibility of not opposing, but connecting, combining and integrating them within research. The
Matović Nataša
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Methodological challenges for collaborative learning research [PDF]

, 2007
Research on collaborative learning, both face-to-face and computer-supported, has thrived in the past 10 years. The studies range from outcome-oriented (individual and group learning) to process-oriented (impact of interaction on learning processes ...
Arnseth, Arvaja, Barron, Beers, De Wever, De Wever, Elliot, Frank Fischer, Hmelo-Silver, Hmelo-Silver, Jan-Willem Strijbos, Kumpulainen, Naidu, Roschelle, Schrire, Stahl, Stahl, Strijbos, Strijbos, Strijbos, Strijbos, Webb, Weinberger, Weinberger, Zemel   +24 more
core   +2 more sources

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Mixed Research Methods Applied to the Gender Perspective in the Press. [PDF]

, 2014
Los métodos mixtos de investigación son cada vez más frecuentes en la investigación en gestión de empresas. Su uso se justifica en tanto que permiten una mayor comprensión de los fenómenos estudiados.
Bezunartea Valencia, Ofa, Caro González, Francisco Javier, García-Gordillo, Mar   +2 more
core   +2 more sources

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Realism and pragmatism in a mixed methods study [PDF]

, 2018
Aim - A discussion of how adopting a Realist rather than Pragmatist methodology affects the conduct of mixed methods research. Background - Mixed methods approaches are now extensively employed in nursing and other health care research.
Bhaskar, Biesta, Biesta, Chakravartty, Feilzer, Giere, Haack, Hillel-Ruben, Kukla, Ladyman, Laudan, Liddell, Machaczek, Maxwell, Mingers, Morgan, Papineau, Pawson, Pawson, Popper, Popper, Putnam, Rorty, Scott, Sosa   +24 more
core   +1 more source

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

A Pilot Study on the Freelisting Method Among Adolescents with Chronic Musculoskeletal Pain: Feasibility, Acceptability and Study Findings

Children
Background/Objectives: To assess the feasibility and acceptability of freelisting for adolescents with chronic musculoskeletal pain (CMP) and use freelisting to identify how adolescents with CMP cope with pain.
Sabrina Gmuca, Dori Abel, Mackenzie McGill, Daneka Stryker, Eda Akpek, Whitney Eriksen, Pamela F. Weiss, Peter F. Cronholm   +7 more
doaj   +1 more source