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Development of Multilocus Sequence Typing (MLST) for Mycoplasma synoviae
Avian Diseases, 2016Mycoplasma synoviae (MS) is a poultry pathogen that has had an increasing incidence and economic impact over the past few years. Strain identification is necessary for outbreak investigation, infection source identification, and facilitating prevention and control as well as eradication efforts.
Mohamed, El-Gazzar +5 more
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Multilocus Sequence Typing (MLST): Markers for the Traceability of Pathogenic Leptospira Strains
2014Leptospirosis is a major zoonosis with worldwide distribution. Conventional serological typing is arduous and time consuming. Genotyping is increasingly applied for the typing and identification of leptospires and contributes to genetic and virulence divergence and molecular epidemiological characteristics such as host versus leptospires population ...
Ahmed, Ahmed +2 more
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Development of a Multilocus sequence typing (MLST) scheme for Pan-Leishmania
Acta Tropica, 2020Since the description of the Leishmania genus, its identification and organization have been a challenge. A high number of molecular markers have been developed to resolve phylogenetic differences at the species level and for addressing key epidemiological and population genetics questions.
Juan Jose, Lauthier +5 more
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Automation of MLST Using Third-Generation Liquid-Handling Technology
Molecular Biotechnology, 2006The molecular characterization of bacterial pathogens of clinical significance is increasingly important. Methods, such as multilocus sequence typing (MLST), allow bacterial strains to be characterized during case clusters, for antibiotic-resistant strains to be monitored, and for the impact of new vaccines to be assessed.
Sullivan, C.B. +3 more
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Development of multilocus sequence typing (MLST) assay for Mycoplasma iowae
Veterinary Microbiology, 2016Mycoplasma iowae (MI) infection is an economically and commercially important disease of turkeys. There are no sequence typing assays available for MI strain identification, the only available molecular tools for this purpose, are DNA fingerprinting assays.
Mostafa, Ghanem, Mohamed, El-Gazzar
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Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus
Veterinary Microbiology, 2019The aim of the investigation was to develop a definitive typing system for Rodentibacter pneumotropicus. A total of 79 strains including the type strain of R. pneumotropicus, all associated with rodents were used to develop a multi-locus sequence typing scheme (MLST). Primers were designed for conserved regions of seven house-keeping genes (atpG, frdB,
Sadhana Adhikary +5 more
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Multilocus Sequence Typing (MLST) for Cronobacter spp.
2017MLST is a molecular typing technique that involves the identification and clustering of bacterial isolates based on the partial sequence analysis of multiple housekeeping genes (generally seven) which are distributed across the length of the genome of the organism. The Cronobacter whole genus MLST scheme can be successfully used for an accurate species
Susan, Joseph, Stephen, Forsythe
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[Multilocus sequence typing (MLST) analysis].
Rinsho byori. The Japanese journal of clinical pathology, 2014Multilocus sequence typing (MLST) analysis has been emerging as a powerful tool for genotyping specific bacterial species. MLST utilizes internal fragments of multiple housekeeping genes and the combination of each allele defines the sequence type for each isolate.
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MLST typing of Acinetobacter baumannii in a hospital outbreak
Pathology, 2023Alfred Lok Hang Lee +2 more
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