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Multilocus Sequence Typing (MLST) of Staphylococcus aureus
2007MLST is a widely accepted method of sequence-based typing that relies on analysis of relatively conserved genes that encode essential proteins. For Staphylococcus aureus the level of discrimination provided by MLST is sufficient to provide a relatively detailed picture of the global dissemination of the pathogen.
Nicholas A, Saunders, Anne, Holmes
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Development of a MLST-biased SNP microarray for Candida albicans
Fungal Genetics and Biology, 2008We have developed a single nucleotide polymorphism (SNP) detection microarray for the human pathogenic yeast, Candida albicans, consisting of synthetic oligonucleotides bound to microscope slides. The array consists of multiple replicates of 79 SNPs, derived from 19 discrete loci located on all eight chromosomes.
Timothy J, Lott, Robin T, Scarborough
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Multilocus Sequence Typing (MLST) of Listeria monocytogenes
2014Nucleotide sequence-based methods focusing on the single nucleotide polymorphisms (SNPs) of Listeria monocytogenes housekeeping genes facilitate the rapid and interlaboratory comparison on open accessible databases, such as the multilocus sequence typing (MLST) databases that are available.
Beatrix, Stessl +2 more
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Development of Multilocus Sequence Typing (MLST) for Mycoplasma synoviae
Avian Diseases, 2016Mycoplasma synoviae (MS) is a poultry pathogen that has had an increasing incidence and economic impact over the past few years. Strain identification is necessary for outbreak investigation, infection source identification, and facilitating prevention and control as well as eradication efforts.
Mohamed, El-Gazzar +5 more
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Multilocus Sequence Typing (MLST): Markers for the Traceability of Pathogenic Leptospira Strains
2014Leptospirosis is a major zoonosis with worldwide distribution. Conventional serological typing is arduous and time consuming. Genotyping is increasingly applied for the typing and identification of leptospires and contributes to genetic and virulence divergence and molecular epidemiological characteristics such as host versus leptospires population ...
Ahmed, Ahmed +2 more
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Development of a Multilocus sequence typing (MLST) scheme for Pan-Leishmania
Acta Tropica, 2020Since the description of the Leishmania genus, its identification and organization have been a challenge. A high number of molecular markers have been developed to resolve phylogenetic differences at the species level and for addressing key epidemiological and population genetics questions.
Juan Jose, Lauthier +5 more
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Development of multilocus sequence typing (MLST) assay for Mycoplasma iowae
Veterinary Microbiology, 2016Mycoplasma iowae (MI) infection is an economically and commercially important disease of turkeys. There are no sequence typing assays available for MI strain identification, the only available molecular tools for this purpose, are DNA fingerprinting assays.
Mostafa, Ghanem, Mohamed, El-Gazzar
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Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus
Veterinary Microbiology, 2019The aim of the investigation was to develop a definitive typing system for Rodentibacter pneumotropicus. A total of 79 strains including the type strain of R. pneumotropicus, all associated with rodents were used to develop a multi-locus sequence typing scheme (MLST). Primers were designed for conserved regions of seven house-keeping genes (atpG, frdB,
Sadhana Adhikary +5 more
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Analysis of Recombination in Campylobacter jejuni from MLST Population Data
Journal of Molecular Evolution, 2005We analyze recombination in C. jejuni using MLST data from isolates taken from wild birds, cattle, wild rabbits, and water in a 100-km2 study region in Cheshire, UK. We use a recent approximate likelihood method for inference, based on combining likelihood information from all pairs of segregating (polymorphic) sites in the data.
Fearnhead, Paul +4 more
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Multilocus Sequence Typing (MLST) for Cronobacter spp.
2017MLST is a molecular typing technique that involves the identification and clustering of bacterial isolates based on the partial sequence analysis of multiple housekeeping genes (generally seven) which are distributed across the length of the genome of the organism. The Cronobacter whole genus MLST scheme can be successfully used for an accurate species
Susan, Joseph, Stephen, Forsythe
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