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Multilocus Sequence Typing (MLST) of Staphylococcus aureus
2007MLST is a widely accepted method of sequence-based typing that relies on analysis of relatively conserved genes that encode essential proteins. For Staphylococcus aureus the level of discrimination provided by MLST is sufficient to provide a relatively detailed picture of the global dissemination of the pathogen.
Nicholas A, Saunders, Anne, Holmes
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Will the emergence of core genome MLST end the role of in silico MLST?
Food Microbiology, 2018The technological advancement of molecular epidemiological analysis using next-generation sequencing (NGS) for foodborne pathogens has a groundbreaking impact over the past three years. In particular, the emergence of cg (core genome) multilocus sequence typing(MLST) has a significant impact.
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Automation of MLST Using Third-Generation Liquid-Handling Technology
Molecular Biotechnology, 2006The molecular characterization of bacterial pathogens of clinical significance is increasingly important. Methods, such as multilocus sequence typing (MLST), allow bacterial strains to be characterized during case clusters, for antibiotic-resistant strains to be monitored, and for the impact of new vaccines to be assessed.
Sullivan, C.B. +3 more
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Development of Multilocus Sequence Typing (MLST) for Mycoplasma synoviae
Avian Diseases, 2016Mycoplasma synoviae (MS) is a poultry pathogen that has had an increasing incidence and economic impact over the past few years. Strain identification is necessary for outbreak investigation, infection source identification, and facilitating prevention and control as well as eradication efforts.
Mohamed, El-Gazzar +5 more
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Multilocus Sequence Typing (MLST) for Cronobacter spp.
2017MLST is a molecular typing technique that involves the identification and clustering of bacterial isolates based on the partial sequence analysis of multiple housekeeping genes (generally seven) which are distributed across the length of the genome of the organism. The Cronobacter whole genus MLST scheme can be successfully used for an accurate species
Susan, Joseph, Stephen, Forsythe
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MLST versus microsatellites for typingAspergillus fumigatusisolates
Medical Mycology, 2009In recent years, there has been a clear and growing tendency to use exact typing methods for discrimination between microbial isolates. Exact typing methods that yield an unambiguous typing result offer a number of advantages over conventional methods in the generation of typing data that is reproducible, portable and exchangeable. Two such methods are
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Multilocus Sequence Typing (MLST): Markers for the Traceability of Pathogenic Leptospira Strains
2014Leptospirosis is a major zoonosis with worldwide distribution. Conventional serological typing is arduous and time consuming. Genotyping is increasingly applied for the typing and identification of leptospires and contributes to genetic and virulence divergence and molecular epidemiological characteristics such as host versus leptospires population ...
Ahmed, Ahmed +2 more
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Development of a MLST-biased SNP microarray for Candida albicans
Fungal Genetics and Biology, 2008We have developed a single nucleotide polymorphism (SNP) detection microarray for the human pathogenic yeast, Candida albicans, consisting of synthetic oligonucleotides bound to microscope slides. The array consists of multiple replicates of 79 SNPs, derived from 19 discrete loci located on all eight chromosomes.
Timothy J, Lott, Robin T, Scarborough
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Development of multi locus sequence typing (MLST) of Rodentibacter pneumotropicus
Veterinary Microbiology, 2019The aim of the investigation was to develop a definitive typing system for Rodentibacter pneumotropicus. A total of 79 strains including the type strain of R. pneumotropicus, all associated with rodents were used to develop a multi-locus sequence typing scheme (MLST). Primers were designed for conserved regions of seven house-keeping genes (atpG, frdB,
Sadhana Adhikary +5 more
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[Multilocus sequence typing (MLST) analysis].
Rinsho byori. The Japanese journal of clinical pathology, 2014Multilocus sequence typing (MLST) analysis has been emerging as a powerful tool for genotyping specific bacterial species. MLST utilizes internal fragments of multiple housekeeping genes and the combination of each allele defines the sequence type for each isolate.
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