Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays [PDF]
AbstractBackgroundMicroRNAs are small non-coding RNAs involved in gene expression regulation by targeting specific regions in the 3′-UTR of the mRNA of their target genes. This binding leads to a decrease in the protein levels of such genes either by mRNA degradation or mRNA destabilization and translation inhibition.
Solé Ferré, Anna +4 more
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Transcript Abundance Explains mRNA Mobility Data in Arabidopsis thaliana [PDF]
Recently, a large population of mRNA was shown to be able to travel between plant organs via sieve elements as a putative long-distance signaling molecule. However, a mechanistic basis by which transcripts are selected for transport has not yet been identified.
Alexander Calderwood +2 more
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Arabidopsis cyclophilins direct plasmodesmata-targeting of mobile mRNA via organelle hitchhiking [PDF]
Abstract Plants selectively transport mobile mRNAs through intercellular pores, plasmodesmata (PD), to distribute spatial information for synchronizing meristematic differentiation with environmental dynamics. However, how plants recognize and deliver mobile mRNAs to PD remains unknown.
Kai-Ren Luo +3 more
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Long-Distance Movement of mRNAs in Plants
Long-distance transport of information molecules in the vascular tissues could play an important role in regulating plant growth and enabling plants to cope with adverse environments.
Chao Xia, Cankui Zhang
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Real-Time Messenger RNA Dynamics in Bacillus subtilis
Messenger RNA molecules have been localized to different positions in cells and have been followed by time-lapse microscopy. We have used MS2-mVenus–labeled mRNA and single-particle tracking to obtain information on the dynamics of single-mRNA molecules ...
Laura Sattler, Peter L. Graumann
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Selective Targeting of Mobile mRNAs to Plasmodesmata for Cell-to-Cell Movement [PDF]
Many plant mRNAs move from cell to cell or long distance to execute non-cell-autonomous functions. These mobile mRNAs traffic through the phloem to regulate many developmental processes, but despite the burgeoning discovery of mobile mRNAs, little is known about the mechanism underlying the intracellular sorting of these mRNAs.
Kai-Ren Luo +2 more
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Group II intron inhibits conjugative relaxase expression in bacteria by mRNA targeting
Group II introns are mobile ribozymes that are rare in bacterial genomes, often cohabiting with various mobile elements, and seldom interrupting housekeeping genes. What accounts for this distribution has not been well understood.
Guosheng Qu +3 more
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Systemic movement of FT mRNA and a possible role in floral induction
FLOWERING LOCUS T (FT) protein is known to be part of the mobile flowering inducing ‘florigen’ signal in plants, but it may not be acting alone. This article reviews the data that FT mRNA can also move systemically throughout the plant and into the shoot
Stephen eJackson, Yiguo eHong
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Mobile FT mRNA contributes to the systemic florigen signalling in floral induction [PDF]
In inducing photoperiodic conditions, plants produce a signal dubbed "florigen" in leaves. Florigen moves through the phloem to the shoot apical meristem (SAM) where it induces flowering. In Arabidopsis, the FLOWERING LOCUS T (FT) protein acts as a component of this phloem-mobile signal.
Li, Chunyang +10 more
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Long-Distance Transport of Prosystemin Messenger RNA in Tomato
Main conclusion: The transcripts of transgenic prosystemin (PS) gene are mobile and the PS mRNA can be translated into protein in tomato and tobacco plants.
Haiyan Zhang, Yuanyuan Hu, Yuanyuan Hu
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