Results 181 to 190 of about 1,079,572 (317)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Feature Selection and Model Optimization for Survival Prediction in Patients with Angina Pectoris. [PDF]

J Clin Med
Bata R, Ghanem AS, Nagy AC.
europepmc   +1 more source

Visible and Near-Infrared Spectroscopy Combined With Bayes Classifier Based on Wavelength Model Optimization Applied to Wine Multibrand Identification. [PDF]

Front Nutr, 2022
Pan T, Li J, Fu C, Chang N, Chen J.
europepmc   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Machine learning model optimization with optuna for accurate prediction of strength and crack behavior in prestressed concrete beams. [PDF]

Sci Rep
Wen Y, Guo R, Duan Z, Tong Y, Tang X, Pan T, Fu C.   +6 more
europepmc   +1 more source

Diagnostic model optimization method for ADHD based on brain network analysis of resting-state fMRI images and transfer learning neural network. [PDF]

Front Hum Neurosci, 2022
Meng X, Zhuo W, Ge P, Zou B, Zhu Y, Liu W, Li X.   +6 more
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

HOES: an efficient multi-evolutionary expert system for deep learning model optimization in time series prediction. [PDF]

Sci Rep
Wei P, Fan C, Yang X, Chen X, Gan J, Deng X, Wei Y, Li Z.   +7 more
europepmc   +1 more source

Study on Multi-Model Soft Sensor Modeling Method and Its Model Optimization for the Fermentation Process of Pichia pastoris. [PDF]

Sensors (Basel), 2021
Wang B, Wang X, He M, Zhu X.
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source