Results 41 to 50 of about 415,608 (282)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Zitterbewegung of optical pulses in nonlinear frequency conversion

, 2010
Pulse walk-off in the process of sum frequency generation in a nonlinear $\chi^{(2)}$ crystal is shown to be responsible for pulse jittering which is reminiscent to the Zitterbewegung (trembling motion) of a relativistic freely moving Dirac particle.
Boyd R W, Greiner W, Maamache M, Schrödinger E, Stefano Longhi, Wang L G   +5 more
core   +2 more sources

Birth of Comments on Atomic, Molecular and Optical Physics [PDF]

Physica Scripta, 2003
With this volume we start a new era for Comments on Atomic and Molecular Physics: subject coverage extended to Optical Physics and our joining Physica Scripta as part of this journal. We are very happy that after the last few years of problems with and changes of publishers we can look forward to a long and fruitful association in this venture.
F J de Heer, H H Stroke
openaire   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Acetaminophen interacts with human hemoglobin: optical, physical and molecular modeling studies [PDF]

Journal of Biomolecular Structure and Dynamics, 2016
Acetaminophen, a widely used analgesic and antipyretic drug has ample affinity to bind globular proteins. Here, we have illustrated a substantive study pertaining to the interaction of acetaminophen with human hemoglobin (HHb). Different spectroscopic (absorption, fluorescence, and circular dichroism (CD) spectroscopy), calorimetric, and molecular ...
Paromita, Seal, Jyotirmoy, Sikdar, Amartya, Roy, Rajen, Haldar   +3 more
openaire   +2 more sources

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Addressing and manipulation of individual hyperfine states in cold trapped molecular ions and application to HD^{+} frequency metrology

, 2012
Advanced techniques for manipulation of internal states, standard in atomic physics, are demonstrated for a charged molecular species for the first time.
Borodin, A., Bressel, U., Ernsting, I., Hansen, M., Schiller, S., Shen, J.   +5 more
core   +1 more source

Understanding bio‐based polymers: A study of origins, properties, biodegradation and their impact on health and the environment

FEBS Open Bio, EarlyView.
This review provides an overview of bio‐based polymer sources, their unique functional properties and their environmental impact, and addresses their role as sustainable alternatives. It discusses end‐of‐life options, including composting and anaerobic digestion for renewable energy.
Sabina Kolbl Repinc, Blaž Stres, Mirica Karlovits, Igor Karlovits, Petra Jerič, Ondrej Panák, Anja Verbič, Blaž Likozar, Uroš Novak   +8 more
wiley   +1 more source

Global and local thermometry schemes in coupled quantum systems

New Journal of Physics, 2017
We study the ultimate bounds on the estimation of temperature for an interacting quantum system. We consider two coupled bosonic modes that are assumed to be thermal and using quantum estimation theory establish the role the Hamiltonian parameters play ...
Steve Campbell, Mohammad Mehboudi, Gabriele De Chiara, Mauro Paternostro   +3 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source