Results 51 to 60 of about 4,989,468 (303)

Array detection enables large localization range for simple and robust MINFLUX

Light: Science & Applications
The MINFLUX concept significantly improves the localization properties of single-molecule localization microscopy (SMLM) by overcoming the limit imposed by the fluorophore’s photon counts.
Eli Slenders, Sanket Patil, Marcus Oliver Held, Alessandro Zunino, Giuseppe Vicidomini   +4 more
doaj   +1 more source

Hyperglycemia and cancer in human lung carcinoma by means of Raman spectroscopy and imaging

Scientific Reports, 2022
Raman spectroscopy and Raman imaging were used to identify the biochemical and structural features of human cancer lung cells (CCL-185) and the cancer cells supplemented with glucose and deuterated glucose at normal and hyperglycemia conditions. We found
M. Kopeć, K. Beton, K. Jarczewska, H. Abramczyk   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

In situ and real-time ultrafast spectroscopy of photoinduced reactions in perovskite nanomaterials

Nature Communications
By employing two synchronized mode-locked femtosecond lasers and interferometric detection of the pump-probe spectra—referred to as asynchronous and interferometric transient absorption—we have developed a method for broad dynamic range and rapid data ...
Gi Rim Han, Mai Ngoc An, Hyunmin Jang, Noh Soo Han, JunWoo Kim, Kwang Seob Jeong, Tai Hyun Yoon, Minhaeng Cho   +7 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Laser scanning reflection-matrix microscopy for aberration-free imaging through intact mouse skull

Nature Communications, 2020
Microscopic imaging of the brain usually requires thinning of the skull, as it causes complex aberration. Here, the authors introduce a label-free imaging modality termed laser scanning reflection-matrix microscopy, which allows for correcting these ...
Seokchan Yoon, Hojun Lee, Jin Hee Hong, Yong-Sik Lim, Wonshik Choi   +4 more
doaj   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

Photonics-based terahertz sources for molecular spectroscopy [PDF]

, 2018
Made available in DSpace on 2018-08-17T16:09:38Z (GMT). No. of bitstreams: 2 3374.pdf: 38157 bytes, checksum: ad069cce1ca7a4384b092101f26197ee (MD5) license.txt: 4802 bytes, checksum: 58353f9dd6876860dd5221f3d7872a95 (MD5) Previous issue date: 6Made
Lampin, Jean-François, Jean-François Lampin   +1 more
core   +1 more source

DEVELOPMENT OF QUANTITATIVE APPLICATIONS FOR MOLECULAR ROTATIONAL RESONANCE SPECTROSCOPY [PDF]

, 2023
The greatest strength of molecular rotational resonance spectroscopy as an analytical technique is its ability to unambiguously resolve the spectral signatures of multiple compounds, including isomers, within a mixture without requiring separation or ...
Byars, Ann Adele
core   +1 more source