Results 71 to 80 of about 424,957 (255)

Analysis of riboflavin/ultraviolet a corneal cross-linking by molecular spectroscopy. [PDF]

Heliyon, 2023
Melcher S, Zimmerer C, Galli R, Golde J, Herber R, Raiskup F, Koch E, Steiner G.   +7 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Label-free differentiation of human pancreatic cancer, pancreatitis, and normal pancreatic tissue by molecular spectroscopy. [PDF]

J Biomed Opt, 2022
Teske C, Kahlert C, Welsch T, Liedel K, Weitz J, Uckermann O, Steiner G.   +6 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Electron heating and thermal relaxation of gold nanorods revealed by two-dimensional electronic spectroscopy

Nature Communications, 2018
Electron dynamics in plasmonic nanoparticles are often studied with transient absorption spectroscopy. Here, Lietard et al. apply two-dimensional electronic spectroscopy, a method generally used for molecular spectroscopy, to study ultrafast nonlinear ...
Aude Lietard, Cho-Shuen Hsieh, Hanju Rhee, Minhaeng Cho   +3 more
doaj   +1 more source

Do Osmolytes Impact the Structure and Dynamics of Myoglobin?

Molecules, 2018
Osmolytes are small organic compounds that can affect the stability of proteins in living cells. The mechanism of osmolytes’ protective effects on protein structure and dynamics has not been fully explained, but in general, two possibilities have ...
Dorota Kossowska, Kyungwon Kwak, Minhaeng Cho   +2 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Removal of back-reflection noise at ultrathin imaging probes by the single-core illumination and wide-field detection

Scientific Reports, 2017
Thin waveguides such as graded-index lenses and fiber bundles are often used as imaging probes for high-resolution endomicroscopes. However, strong back-reflection from the end surfaces of the probes makes it difficult for them to resolve weak contrast ...
Changhyeong Yoon, Munkyu Kang, Jin H. Hong, Taeseok D. Yang, Jingchao Xing, Hongki Yoo, Youngwoon Choi, Wonshik Choi   +7 more
doaj   +1 more source

A molecular spectroscopy approach for the investigation of early phase ochronotic pigment development in Alkaptonuria. [PDF]

Sci Rep, 2021
Bernini A, Petricci E, Atrei A, Baratto MC, Manetti F, Santucci A.   +5 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source