Results 101 to 110 of about 2,692,899 (243)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Identifying events that impact self-efficacy in physics learning
Physical Review Special Topics. Physics Education Research, 2012 We present a method of analyzing the development of self-efficacy in real time using a framework of self-efficacy opportunities (SEOs). Considerable research has shown a connection between self-efficacy, or the confidence in one’s own ability to perform ...
Vashti Sawtelle +3 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Nuclear Physics BWe revisit the Simple Extension of the Standard Model (SESM), which can account for various tentative new physics anomalies and dark matter. The B physics anomalies, muon anomalous magnetic moment, and dark matter can be explained already in SESM.
Tianjun Li +3 more
doaj +1 more source
Physics goals of a μ+μ− collider
, 1996 This working group report focuses on the physics potential of μ+μ− colliders beyond what can be accomplished at linear e+e− colliders and the LHC. Particularly interesting possibilities include (i) s‐channel resonance production to discover and study ...
Willis, B. +11 more
core +1 more source
Pendulums and Nonlinear Effects - Warning 4000 level physics [video]
, 2015 NPS PhysicsIn-class Physics DemonstrationsDr.
Naval Postgraduate School Physics
core +2 more sources
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Search for new physics in events with same-sign dileptons and jets in pp collisions at ?s = 8 TeV
, 2014 A search for new physics is performed based on events with jets and a pair of isolated, same-sign leptons. The results are obtained using a sample of proton-proton collision data collected by the CMS experiment at a centre-of-mass energy of 8 TeV at the ...
Physics Collaboration, CMS Collaboration
core +1 more source