Results 51 to 60 of about 174,983 (236)

Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential

Advanced Healthcare Materials, EarlyView.
This review examines how cellular behavior is regulated by mechanical cues transmitted through soft biomaterials, from single‐cell mechanosensing to tissue‐level adaptation. It highlights why physiological relevance, rather than model complexity alone, is critical for translational mechanobiology and introduces a scoring framework linking material ...
Mathias Polz, Manuel P. Kainz, Niklas Haider, Theresa Rienmüller, Kerstin Lenk, Rupert Kargl, Michele Terzano, Gerhard Sommer, Gerhard A. Holzapfel, Julia Fuchs   +9 more
wiley   +1 more source

A 3D‐Printed Vascular‐Like Perfusion Tumor‐on‐a‐Chip Recapitulates High‐Grade Breast Cancer With in Vivo‐Relevant Drug Resistance

Advanced Healthcare Materials, EarlyView.
A high‐resolution 3D‐printed tumor‐on‐a‐chip platform sustains breast cancer tissues at in vivo–like cell densities through vascular‐like perfusion. This engineered system recapitulates grade‐dependent molecular features and exhibits drug‐resistance profiles that uniquely align with clinically relevant Cmax values.
Geonhui Lee, Bingjie Sun, Nicholas X. Fang, Chunguang Xia   +3 more
wiley   +1 more source

Electrical maturation of neurons derived from human embryonic stem cells [v1; ref status: indexed, http://f1000r.es/42n]

F1000Research, 2014
In-vitro neuronal differentiation of human pluripotent stem cells has become a widely used tool in disease modeling and prospective regenerative medicine. Most studies evaluate neurons molecularly and only a handful of them use electrophysiological tools
Michael Telias, Menahem Segal, Dalit Ben-Yosef   +2 more
doaj   +1 more source

A Fully Defined GelMA‐Based Matrix Allows Fine Tuning of Tissue‐Relevant Biomechanical and Biochemical Cues for Organoid Culture

Advanced Healthcare Materials, EarlyView.
A fully defined GelMA‐based ECM platform integrates tunable tissue‐relevant stiffness with laminin–entactin biochemical supplementation to support mouse small intestinal organoid growth. Coordinated control of mechanical and biochemical cues promotes robust proliferation, budding morphogenesis, differentiation, and passaging, providing a reproducible ...
Junyi Liu, Su Su Htwe, Lay Poh Tan, Yen Choo   +3 more
wiley   +1 more source

Initiation of lumen formation from junctions via differential actomyosin contractility regulated by dynamic recruitment of Rasip1

Nature Communications
De novo lumen formation necessitates the precise segregation of junctional proteins from apical surfaces, yet the underlying mechanisms remain unclear. Using a zebrafish model, we develop a series of molecular reporters, photo-convertible and optogenetic
Jianmin Yin, Niels Schellinx, Ludovico Maggi, Kathrin Gundel, Cora Wiesner, Maria Paraskevi Kotini, Minkyoung Lee, Li-Kun Phng, Heinz-Georg Belting, Markus Affolter   +9 more
doaj   +1 more source

Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections

iScience, 2019
Summary: Intercellular contacts are essential for precise organ morphogenesis, function, and maintenance; however, spatiotemporal information of cell-cell contacts or adhesions remains elusive in many systems.
Nagatoki Kinoshita, Arthur J.Y. Huang, Thomas J. McHugh, Sachihiro C. Suzuki, Ichiro Masai, Il Hwan Kim, Scott H. Soderling, Atsushi Miyawaki, Tomomi Shimogori   +8 more
doaj   +1 more source

Tomographic Printing in a Chip: A Versatile Platform for Biomimetic 3D Organ‐on‐Chip

Advanced Healthcare Materials, EarlyView.
A novel light‐based bioprinting approach, TVAM‐in‐a‐chip, enables rapid, contactless fabrication of biomimetic 3D organ‐on‐chip devices directly within preassembled microfluidic platforms. This strategy supports diverse materials, including cell‐laden resins, and eliminates post‐assembly steps.
Riccardo Rizzo, Viola Sgarminato, Felix Wechsler, Christophe Moser   +3 more
wiley   +1 more source

Applying mechanical forces on Drosophila tissues in vivo using the StretchCo, a 3D-printable device

STAR Protocols
Summary: Applying mechanical forces to tissues helps to understand morphogenesis and homeostasis. Additionally, recording the dynamics of living tissues under mechanical constraints is needed to explore tissue biomechanics. Here, we present a protocol to
Mélanie Gracia, Bénédicte M. Lefèvre, Raquel Güell Alonso, Victoire Cachoux, Maria Balakireva, Boris Guirao, Damien Cuvelier, Allison J. Bardin, Yohanns Bellaïche   +8 more
doaj   +1 more source

Electrical maturation of neurons derived from human embryonic stem cells [v2; ref status: indexed, http://f1000r.es/4gy]

F1000Research, 2014
In-vitro neuronal differentiation of human pluripotent stem cells has become a widely used tool in disease modeling and prospective regenerative medicine. Most studies evaluate neurons molecularly and only a handful of them use electrophysiological tools
Michael Telias, Menahem Segal, Dalit Ben-Yosef   +2 more
doaj   +1 more source

Myosin-18B Promotes Mechanosensitive CaMKK2-AMPK-VASP Regulation of Contractile Actin Stress Fibers

iScience, 2020
Summary: Actin stress fibers guide cell migration and morphogenesis. During centripetal flow, actin transverse arcs fuse accompanied by the formation of myosin II stacks to generate mechanosensitive actomyosin bundles.
Shuangshuang Zhao, Xuemeng Shi, Yue Zhang, Zeyu Wen, Jinping Cai, Wei Gao, Jiayi Xu, Yifei Zheng, Baohua Ji, Yanqin Cui, Kun Shi, Yanjun Liu, Hui Li, Yaming Jiu   +13 more
doaj   +1 more source