Results 81 to 90 of about 199,308 (309)

Intelligent Stain‐Free Histology on Structural Colorimetric Nanocavities

Advanced Science, EarlyView.
Chen and Gan et al. introduce a label‐free imaging platform using inexpensive Nanocavity‐on‐Silicon (NOS) slides. These slides transform subtle tissue variations into vivid structural colors, enabling high‐contrast histological imaging under a regular optical microscope. This stain‐free approach reveals morphological details comparable to traditional H&
Qizhe Chen, Yifei Ren, Lijie Hu, Yanyan Li, Wenyue Liang, Jin Wang, Han Gao, Xinhai Wang, Jiajun Li, Qiutao He, Yingfeng Zhu, Haifeng Hu, Qiwen Zhan, Imed Gallouzi, Jasmeen Merzaban, Di Wang, Zunguo Du, Xiaodong Gu, Qiaoqiang Gan   +18 more
wiley   +1 more source

Allogeneic Immune Cell Perfusion Inhibits the Growth of Vascularized 3D In Vitro Tumor Models, Induces Vascular Regression and Desmoplasia, but Promotes Tumor Cell Invasion

Advanced Science, EarlyView.
This study presents a vascularized 3D tumor model to investigate immune–stromal–tumor interactions under allogeneic PBMC perfusion. While immune cells induced tumor shrinkage, they also promoted vascular regression, stromal activation, and cancer cell invasion.
Alexandra Raab, Rasika Daware, Marcelo A. Szymanski de Toledo, Oskar Weber, Dimitris Kapsokalyvas, Twan Lammers, Horst Fischer, Federica De Lorenzi, Fabian Kiessling   +8 more
wiley   +1 more source

Autophagy Activators Normalize Aberrant Tau Proteostasis and Rescue Synapses in Human Familial Alzheimer's Disease iPSC‐Derived Cortical Organoids

Advanced Science, EarlyView.
A new cerebrocortical organoid model using isogenic hiPSCs with familial Alzheimer's mutations recapitulates key AD features, including amyloid‐beta and phospho‐Tau aggregation, neuronal hyperexcitability, and synapse loss. Single‐cell RNA‐seq reveals aberrant pathways in excitatory and inhibitory neurons.
Sergio R. Labra, Jadon Compher, Akhil Prabhavalkar, Mireya Almaraz, Claudia Cedeño Kwong, Christine Baal, Maria Talantova, Nima Dolatabadi, Julian Piña‐Sanz, Yubo Wang, Leonard Yoon, Swagata Ghatak, Zi Gao, Yuting Zhang, Dorit Trudler, Lynee Massey, Wei Lin, Anthony Balistreri, Michael Bula, Nicholas J. Schork, Tony S. Mondala, Steven R. Head, Jeffery W. Kelly, Stuart A. Lipton   +23 more
wiley   +1 more source

Ovarian reserve evaluation in a woman with 45,X/47,XXX mosaicism: A case report and a review of literature

Molecular Genetics & Genomic Medicine, 2019
Background Turner syndrome (TS) is a common chromosomal disorder affecting approximately 1:2,500 live female births. Mosaic 47,XXX karyotype is found in 3%–4% of TS patients.
Ruiyi Tang, Lin Lin, Zaixin Guo, Haiyan Hou, Qi Yu   +4 more
doaj   +1 more source

Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation

Nature Communications, 2020
Injecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles.
Yanhe Li, Yuteng Weng, Dandan Bai, Yanping Jia, Yingdong Liu, Yalin Zhang, Xiaochen Kou, Yanhong Zhao, Jingling Ruan, Jiayu Chen, Jiqing Yin, Hong Wang, Xiaoming Teng, Zuolin Wang, Wenqiang Liu, Shaorong Gao   +15 more
doaj   +1 more source

Genomic mosaicism in paternal sperm and multiple parental tissues in a Dravet syndrome cohort

Scientific Reports, 2017
Genomic mosaicism in parental gametes and peripheral tissues is an important consideration for genetic counseling. We studied a Chinese cohort affected by a severe epileptic disorder, Dravet syndrome (DS).
Xiaoxu Yang, Aijie Liu, Xiao-jing Xu, Xiaoling Yang, Q. Zeng, A. Y. Ye, Zhe Yu, Sheng Wang, A. Huang, Xi-ru Wu, Qixi Wu, Liping Wei, Yuehua Zhang   +12 more
semanticscholar   +1 more source

NuSAP Safeguards Centriole Integrity to Mediate CEP57‐CEP152 Torus Recruitment for Proper Engagement

Advanced Science, EarlyView.
This study reveals a novel role for the microtubule stabilizer NuSAP at centrioles. NuSAP depletion destabilizes the centriole's tubulin structure, causing premature disengagement, PCM defects, and mis‐localization of the CEP57‐CEP63‐CEP152 complex. By reinforcing centriole architecture, NuSAP enables early CEP57 loading and initiates a newly proposed ...
Shiyu Zhang, Zemin Jiang, Qiaoyun Yang, Hong Zheng, Minghao Wang, Chennianci Zhu, Lih‐Wen Deng, Karen Carmelina Crasta, Yih‐Cherng Liou   +8 more
wiley   +1 more source

Embedded CRISPRi Enhances Gene‐Silencing Efficiency in Drosophila

Advanced Science, EarlyView.
Current CRISPR interference (CRISPRi) technology in Drosophila has limited efficiency. This study introduces the emCRISPRi platform, which significantly enhances transcriptional silencing efficacy by embedding inhibitory domains within the dCas9 architecture.
Pengchong Fu, Xuedi Zhang, Ying Zhou, Jie Zheng, Angyang Sun, Kailong Zhuang, Weiwei Bao, Guanjun Gao   +7 more
wiley   +1 more source

A Testis‐Specific Aralkylamine N‐Acetyltransferase Regulates Dimorphic Sperm Function and Male Fertility in Moths

Advanced Science, EarlyView.
We identify a Lepidoptera‐conserved testis‐specific arylalkylamine N‐acetyltransferase (LTNAT) that governs male moth fertility via a novel mechanism. LTNAT loss disrupts eupyrene sperm mitochondrial derivatives and impairs apyrene sperm motility, offering a safe molecular target for innovative pesticides and genetic pest control.
Hao Sun, Peng‐Yi Huang, Zhi‐Ruo Zhang, Cong‐Fen Gao, Subba Reddy Palli, Shun‐Fan Wu   +5 more
wiley   +1 more source

Detecting Genetic Mosaicism in Cultures of Human Pluripotent Stem Cells

Stem Cell Reports, 2016
Summary Genetic changes in human pluripotent stem cells (hPSCs) gained during culture can confound experimental results and potentially jeopardize the outcome of clinical therapies. Particularly common changes in hPSCs are trisomies of chromosomes 1, 12,
D. Baker, Adam J. Hirst, P. Gokhale, Miguel A. Juárez, S. Williams, M. Wheeler, Kerry Bean, T. Allison, H. Moore, P. Andrews, Ivana Barbaric   +10 more
semanticscholar   +1 more source