Results 171 to 180 of about 597,542 (311)

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

The impact of supplementation with calcium and naloxone to TRIS and Lepus extenders on the quality of refrigerated rabbit semen. [PDF]

Front Vet Sci
Zappone V, Alonge S, Angelastri R, Troisi A, Marcoccia R, Quartuccio M, Aiudi GG.   +6 more
europepmc   +1 more source

Actin-based cell motility and cell locomotion.

Cell, 1996
T. Mitchison, L. Cramer
semanticscholar   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Immediate and sustained resolution of chronic post-traumatic diplopia following lower eyelid adhesiolysis using plasma energy (Plexr): An 8-month follow-up case report. [PDF]

Trauma Case Rep
Tsioumas SG, Sagaro GG, Ricci G, Bellitto V, Nittari G.   +4 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Mfd interacts with RNA polymerase to modulate flagellum-dependent motility, chemotaxis, and population heterogeneity in the stationary phase of <i>Bacillus subtilis</i>. [PDF]

J Bacteriol
Grifaldo J, Leyva-Sanchez H, Rosales K, Callaway A, Sotelo A, Martin HA, Perez RK, Chavez Rios J, Huezo EN, Pedraza-Reyes M, Robleto EA.   +10 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Functional Characterisation of the Quorum-Sensing Regulator ExpR_Ecz in Modulation of Dickeya oryzae Motility and Virulence. [PDF]

Mol Plant Pathol
Liang Z, Liu H, He L, Chen Z, Gu W, Wu X, Chen Q, Wang X, Zhang LH.   +8 more
europepmc   +1 more source