Results 11 to 20 of about 9,800 (208)

Nanoscopic surfactant behavior of the porin MspA in aqueous media [PDF]

Beilstein Journal of Nanotechnology, 2013
The mycobacterial porin MspA is one of the most stable channel proteins known to date. MspA forms vesicles at low concentrations in aqueous buffers. Evidence from dynamic light scattering, transmission electron microscopy and zeta-potential measurements ...
Ayomi S. Perera, Hongwang Wang, Tej B. Shrestha, Deryl L. Troyer, Stefan H. Bossmann   +4 more
doaj   +4 more sources

Control of subunit stoichiometry in single-chain MspA nanopores [PDF]

Biophysical Journal, 2021
ABSTRACTTransmembrane protein channels enable fast and highly sensitive electrical detection of single molecules. Nanopore sequencing of DNA was achieved using an engineered Mycobacterium smegmatis porin A (MspA) in combination with a motor enzyme. Due to its favorable channel geometry, the octameric MspA pore exhibits the highest current level as ...
Mikhail Pavlenok, Luning Yu, Dominik Herrmann, Meni Wanunu, Michael Niederweis   +4 more
openaire   +2 more sources

Channel Blocking of MspA Revisited [PDF]

Langmuir, 2012
Porin A from Mycobacterium smegmatis (MspA) is a highly stable, octameric channel protein, which acts as the main transporter of electrolytes across the cell membrane. MspA features a narrow, negatively charged constriction zone, allowing stable binding of various analytes thereby blocking the channel.
Perera, Ayomi S., Wang, Hongwang, Basel, Matthew T., Pokhrel, Megh Raj, Gamage, Pubudu Siyambalagoda, Kalita, Mausam, Wendel, Sebastian, Sears, Bryan, Welideniya, Dhanushi, Liu, Yao, Turro, Claudia, Troyer, Deryl L., Bossmann, Stefan H.   +12 more
openaire   +2 more sources

Nanopore Sequencing with MspA [PDF]

Biophysical Journal, 2009
Nanopores may provide the basis for a high-speed and inexpensive de novo DNA sequencing technique that could revolutionize medical and biological science. In this technique, single-stranded DNA is electrophoretically translocated through a pore with inner dimensions similar to that of DNA.
Derrington, Ian, Butler, Tom, Pavlenok, M., Collins, Marcus, Niederweis, Michael, Gundlach, Jens   +5 more
openaire   +1 more source

Thermal Motion of DNA in an MspA Pore [PDF]

Biophysical Journal, 2015
ABSTRACTWe report on an experiment and calculations that determine the thermal motion of a voltage-clamped ssDNA-NeutrAvidin complex in an MspA nanopore. The electric force and diffusion constant of DNA inside an MspA pore have been determined in order to evaluate DNA’s thermal position fluctuations.
Lu, Bo, Fleming, Stephen Jordan, Szalay, Tamas, Golovchenko, Jene   +3 more
openaire   +3 more sources

Continuously Scanning DNA with Nanopore MspA [PDF]

Biophysical Journal, 2016
Nanopore sequencing is a promising next-generation technology that is being enabled by the biological nanopore MspA. In this sequencing technique, current is driven through the ∼1 nm constriction of MspA. Single stranded DNA molecules are first drawn into the pore by an applied voltage.
Noakes, Matthew T., Derrington, Ian M., Langford, Kyle W., Brinkerhoff, Henry D., Manrao, Elizabeth, Laszlo, Andrew H., Bartlett, Joshua, Tickman, Benjamin I., Blum, Jackie, Gundlach, Jens H.   +9 more
openaire   +1 more source

Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransporters [PDF]

, 2015
Neisseria meningitidis, a major cause of bacterial meningitis and septicaemia, secretes multiple virulence factors, including the adhesion and penetration protein (App) and meningococcal serine protease A (MspA).
Aisen, Ait-Tahar, Benabdillah, Benz, Bhavsar, Celik, Demogines, Duncan, Füllgrabe, Gerace, Hadi, Haraga, Hashino, Hayes, Henderson, Hill, Ilver, Johansson, Johansson, Khorasanizadeh, Koomey, Lange, Leyton, Linehan, Luger, Macedo-Ramos, Marfori, Martin, Martinez-Pomares, Martinez-Pomares, Matangkasombut, McSweeney, Mrkic, Oldfield, Park, Parker, Pohlner, Poulsen, Rawlings, Robinson, Rothbart, Ruiz-Perez, Sallusto, Santos-Rosa, Serruto, Sjölinder, St Geme, Stephens, Strahl, Syed, Taylor, Tsuruta, Turner, Turner, Ulsen, Ulsen, Ulsen, Van Noorden, Virji, Vliet, Wang, Zamze, Zhang   +62 more
core   +5 more sources

Biological Nanopore MspA for DNA Sequencing [PDF]

Biophysical Journal, 2012
Nanopore sequencing is a single molecule technique that has the potential to provide fast and low-cost DNA sequencing. In nanopore sequencing, an ionic current passing through a small pore would directly map the sequence as single stranded DNA is driven through the constriction.
Manrao, Elizabeth A., Derrington, Ian M., Langford, Kyle W., Pavlenok, Mikhail, Niederweis, Michael, Gundlach, Jens H.   +5 more
openaire   +1 more source

Hetero-oligomeric MspA pores inMycobacterium smegmatis [PDF]

FEMS Microbiology Letters, 2016
The porin MspA of Mycobacterium smegmatis is a biological nanopore used for DNA sequencing. The octameric MspA pore can be isolated from M. smegmatis in milligram quantities, is extremely stable against denaturation and rapidly inserts into lipid membranes. Here, we show that MspA pores composed of different Msp subunits are formed in M.
Mikhail, Pavlenok, Michael, Niederweis
openaire   +2 more sources

Engineering Biological Nanopore MspA for Sequencing DNA [PDF]

Biophysical Journal, 2011
The sequence of a long DNA molecule can be determined, in principle, by measuring the ionic current blockade the molecule produces as it permeates a nanometer-diameter pore in a thin insulating membrane. The difficulties in realizing this idea in practice are common to both biological and synthetic nanopores: the pore geometry does not permit isolation
Aksimentiev, Aleksei, Bhattacharya, Swati, Ho, Anthony   +2 more
openaire   +1 more source