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Cell Sensitivity Assays: The MTT Assay
2003The clonogenic assay described in Chapter 2 is not suitable for all cell lines. Many adherent cell lines do not form colonies and clearly it is not applicable to non-adherent cell lines. Furthermore, it is slow and time consuming. This chapter describes an alternative cytotoxicity assay that has a number of advantages when compared with a clonogenic ...
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Oncology, 1994
The MTT assay in agarose, a simple colorimetric test performed in double-layer agarose, has been evaluated for chemosensitivity testing of fresh tumor samples from human cancers in comparison with the MTT assay. The absorbance of cells from fibroblast cell lines or normal tissues was markedly reduced in agarose.
R, Abe, H, Ueo, T, Akiyoshi
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The MTT assay in agarose, a simple colorimetric test performed in double-layer agarose, has been evaluated for chemosensitivity testing of fresh tumor samples from human cancers in comparison with the MTT assay. The absorbance of cells from fibroblast cell lines or normal tissues was markedly reduced in agarose.
R, Abe, H, Ueo, T, Akiyoshi
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The mitochondrial uncoupler dicumarol disrupts the MTT assay
Biochemical Pharmacology, 2003Dicumarol is routinely added to the 3-[4,4-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay to study the role of NAD(P)H:quinone oxido-reductase in drug activation and detoxification. We assessed the direct impact of dicumarol (a mitochondrial uncoupler) on the MTT assay. Mouse mammary tumor (EMT6) and Chinese hamster ovary (CHO) cells
Abby C, Collier, Chris A, Pritsos
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Measurement of radiation survival using the MTT assay
European Journal of Cancer, 1994There is increasing interest in the development of rapid assays of radiosensitivity which can be used on clinical specimens. Unfortunately, the measurement of radiation survival using clonogenic assays, which are the established standard, can be difficult and time consuming.
A, Slavotinek, T J, McMillan, C M, Steel
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Analysis of Cell Viability by the MTT Assay
Cold Spring Harbor Protocols, 2018Among viability assays that depend on the conversion of substrate to chromogenic product by live cells, the MTT assay is still among one of the most versatile and popular assays. The MTT assay involves the conversion of the water-soluble yellow dye MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to an insoluble purple formazan by the
Priti, Kumar +2 more
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Liposomes and MTT cell viability assay: An incompatible affair
Toxicology in Vitro, 2015The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is commonly used to evaluate the cytotoxicity potential of drugs vehicled by liposomes. However, liposome delivering drugs could produce inconsistent values of MTT absorbance.
ANGIUS, FABRIZIO, Floris A.
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The MTT Assay to Evaluate Chemosensitivity
2005The assessment of the degree or rate of cellular proliferation and cell viability is critical to the assessment of the effects of drugs, antibodies, or cytokines on both normal and malignant cell populations. This can be accomplished by either direct or indirect counting methods.
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Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin
2017This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures. The underlying principle of these assays is the measurement of a biochemical marker to evaluate the cell's metabolic activity. The formation of the omnipresent reducing agents NADH and NADPH is used as a marker for metabolic activity in ...
Konstantin, Präbst +3 more
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Use of a filtration plate in the MTT assay
Toxicology in Vitro, 1994The use of the MultiScreen filtration plate enables fluids to be removed efficiently from the plate without disturbing cells, or formazan crystals formed during the MTT test. Using a 1:1 mix of dimethyl sulfoxide and ethanol as solvent allows optical densities to be measured directly on the Multiscreen plate.
D, Sladowski +3 more
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MTT Growth Assays in Ovarian Cancer
2003The MTT (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide) growth assay developed by Mosmann (1) offers a simple, rapid, and precise measurement of cell viability and proliferation of adherent cell lines (2). The value of this assay is in the screening of large numbers of samples. The MTT assay, a quantitative colorimetric assay is based on
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