Results 81 to 90 of about 919,462 (268)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Energy-absorbing structures with multi-material topology optimization considering large deformation

Nihon Kikai Gakkai ronbunshu
Future vehicle design will require lightweight, crashworthy, and stiffness, yet it will balance these requirements. A promising approach, multi-material topology optimization, can reasonably place solid materials with different mechanical properties ...
Daiki WATANABE, Masatoshi SHIMODA
doaj   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Stimuli of 4D technology and its impact on architectural design in the age of artificial intelligence [PDF]

Journal of Engineering Science and Military Technologies
The paper discusses the advancements in 4D printing applications inarchitecture; the concept of 4D printing has revolutionized the field ofarchitecture by introducing a new dimension to the construction process.Although it is based on 3D printing ...
Haytham Elbadrawy, Marina Abdelmasih
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

A Multi-Material Self-Healing Soft Gripper

2019 2nd IEEE International Conference on Soft Robotics (RoboSoft), 2019
In the field of soft robotics, the material selection plays an important role and markedly influences the properties of the actuators. More complex actuators can be manufactured by combining the strengths of multiple materials in a single design. To allow this, a good connection between the different materials is indispensable.
Ellen Roels, Seppe Terryn, Joost Brancart, Guy Van Assche, Bram Vanderborght   +4 more
openaire   +2 more sources

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Mechanical behavior of macroscopic interfaces for 3D printed multi-material samples made of dissimilar materials

Mechanics & Industry
The development of 3D printing technologies has introduced new possibilities in producing multi-material parts, and Fused Filament Fabrication (FFF) is one of these technologies.
Ermolai Vasile, Sover Alexandru, Boca Marius Andrei, Mihalache Andrei Marius, Irimia Alexandru Ionuț, Hrițuc Adelina, Slătineanu Laurențiu, Nagîț Gheorghe, Stavarache Răzvan Cosmin   +8 more
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source