Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components. [PDF]
Free Radic Biol Med, 2015 Individual recombinant components of pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes (PDHc, OGDHc) of human and Escherichia coli (E. coli) origin were expressed and purified from E. coli with optimized protocols.
Ambrus A +6 more
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Richard Nelson Perham. 27 April 1937—14 February 2015 [PDF]
, 2018 Richard Nelson Perham, FRS, FMedSci, FRSA, was a British professor of structural biochemistry. He undertook his academic career at the University of Cambridge, holding positions as lecturer, reader, chair and head of the Department of Biochemistry, as ...
Charoy, François +6 more
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Biomolecules, 2021 The use of multienzyme complexes can facilitate biocatalytic cascade reactions by employing fusion enzymes or protein tags. In this study, we explored the use of recently developed peptide tags that promote complex formation of the targeted proteins: the
Ni Nyoman Purwani +3 more
doaj +1 more source
Metabolic Fuel for Epigenetic: Nuclear Production Meets Local Consumption
Frontiers in Genetics, 2021 Epigenetic modifications are responsible for finetuning gene expression profiles to the needs of cells, tissues, and organisms. To rapidly respond to environmental changes, the activity of chromatin modifiers critically depends on the concentration of a ...
Ruben Boon, Ruben Boon, Ruben Boon
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Exogenous dietary enzyme formulations improve growth performance of broiler chickens fed a low-energy diet targeting the intestinal nutrient transporter genes. [PDF]
PLoS ONE, 2018 Diminishing the cost of broiler chicken diet is a critical issue in the poultry industry. Numerous studies were performed to achieve this pivotal objective by diet supplementation with alternative feed additives.
Ahmed A Saleh +5 more
doaj +1 more source
Variation in the organization and subunit composition of the mammalian pyruvate dehydrogenase complex E2/E3BP core assembly [PDF]
, 2011 The final version of this article is available at the link below.Crucial to glucose homoeostasis in humans, the hPDC (human pyruvate dehydrogenase complex) is a massive molecular machine comprising multiple copies of three distinct enzymes (E1–E3) and an
Alan Cooper +44 more
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Non-translational Connections of eEF1B in the Cytoplasm and Nucleus of Cancer Cells
Frontiers in Molecular Biosciences, 2020 The human translation machinery includes three types of supramolecular complexes involved in elongation of the polypeptide chain: the ribosome, complex of elongation factors eEF1B and multienzyme aminoacyl-tRNA synthetase complex.
Boris Negrutskii
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Research progress of multi-enzyme complexes based on the design of scaffold protein
Bioresources and Bioprocessing, 2023 Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways.
Xiangyi Wang +5 more
doaj +1 more source
Performance and economic viability of broiler chicken fed diets with multienzyme complexes
Acta Scientiarum: Animal Sciences, 2017 This study evaluated the effect of multienzyme complexes (EC1 and EC2) in low nutritional density diets on performance and economic viability of broilers. A total of 840 day-old chicks were assigned to 7 treatments, distributed in a completely randomized
Guilherme Aguiar Mateus Pasquali +5 more
doaj +3 more sources
Human 2-Oxoglutarate Dehydrogenase Complex E1 Component Forms a Thiamin-derived Radical by Aerobic Oxidation of the Enamine Intermediate. [PDF]
, 2014 Herein are reported unique properties of the human 2-oxoglutarate dehydrogenase multienzyme complex (OGDHc), a rate-limiting enzyme in the Krebs (citric acid) cycle.
Ambrus, Attila +5 more
core +4 more sources