Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components. [PDF]
Free Radic Biol Med, 2015 Individual recombinant components of pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes (PDHc, OGDHc) of human and Escherichia coli (E. coli) origin were expressed and purified from E. coli with optimized protocols.
Ambrus A +6 more
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How evolution dismantles and reassembles multienzyme complexes. [PDF]
Proc Natl Acad Sci U S A, 2022 The mycobacterial PDH features an unusual architecture that may allow it to function in combination with otherα-ketoacid dehydrogenase complexes.
Mattevi A.
europepmc +8 more sources
Low immunogenicity of the common lipoamide dehydrogenase subunit (E3) of mammalian pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multienzyme complexes [PDF]
Biochemical Journal, 1985 The production of high-titre monospecific polyclonal antibodies against the purified pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multienzyme complexes from ox heart is described.
Olga L. De Marcucci +2 more
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Regulation of Brain Pyruvate Dehydrogenase Multienzyme Complex [PDF]
Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques, 1978 SUMMARY:A number of excellent and comprehensive reviews on various aspects of pyruvate dehydrogenase multienzyme complex have been written recently. The purpose of the present review is to summarize briefly the reaction mechanism and the regulation of this enzyme. Emphasis is put on the most recent literature not covered by previous reviews. Particular
T. T. Ngo, A. Barbeau
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Structure of the murine lysosomal multienzyme complex core [PDF]
Science Advances, 2021 The 3D structure of the complex formed by the enzymes GLB1 and CTSA furthers our understanding of lysosomal storage diseases.
A. Gorelik +4 more
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Knockout studies reveal an important role of Plasmodium lipoic acid protein ligase A1 for asexual blood stage parasite survival. [PDF]
PLoS ONE, 2009 Lipoic acid (LA) is a dithiol-containing cofactor that is essential for the function of alpha-keto acid dehydrogenase complexes. LA acts as a reversible acyl group acceptor and 'swinging arm' during acyl-coenzyme A formation.
Svenja Günther +2 more
doaj +10 more sources
Composition and conservation of the mRNA-degrading machinery in bacteria [PDF]
Journal of Biomedical Science, 2011 RNA synthesis and decay counteract each other and therefore inversely regulate gene expression in pro- and eukaryotic cells by controlling the steady-state level of individual transcripts.
Kaberdin Vladimir R +2 more
doaj +6 more sources
Multienzyme complex for metabolic channeling in mammalian DNA replication. [PDF]
Proceedings of the National Academy of Sciences, 1980 In the DNA-synthesizing phase (S phase) of CHEF/18 Chinese hamster embryo fibroblast cells, six enzymes associated with DNA metabolism, including DNA polymerase (deoxynucleoside triphosphate:DNA deoxynucleotidyl-transferase, EC 2.7.7.7), were largely localized in the nuclear region (karyoplasts).
G. Prem Veer Reddy, Arthur B. Pardee
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Multienzyme complexes of eukaryotic aminoacyl-tRNA synthetases [PDF]
Bioscience Reports, 1983 The high-molecular-mass aminoacyl-tRNA synthetase complexes in higher eukaryotes are discrete non-artifactual multienzyme complexes of similar composition which can be purified from different tissues of different species by different techniques. These high-Mr complexes are composed of synthetases in simple stoichiometric ratios.
Chi V. Dang, Chuan V. Dang
openaire +3 more sources
Performance and economic viability of broiler chicken fed diets with multienzyme complexes [PDF]
Acta Scientiarum: Animal Sciences, 2017 This study evaluated the effect of multienzyme complexes (EC1 and EC2) in low nutritional density diets on performance and economic viability of broilers. A total of 840 day-old chicks were assigned to 7 treatments, distributed in a completely randomized
Guilherme Aguiar Mateus Pasquali +5 more
doaj +6 more sources