How evolution dismantles and reassembles multienzyme complexes [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2022 The mycobacterial PDH features an unusual architecture that may allow it to function in combination with otherα-ketoacid dehydrogenase complexes.
Andrea Mattevi
exaly +6 more sources
Fine tuning of spatial arrangement of enzymes in a PCNA-mediated multienzyme complex using a rigid poly-L-proline linker. [PDF]
PLoS ONE, 2013 Inspired by natural multienzyme complexes, many types of artificial multienzyme complexes have recently been constructed. We previously constructed a self-assembled complex of a bacterial cytochrome P450 and its ferredoxin and ferredoxin reductase ...
Tomoaki Haga +2 more
doaj +2 more sources
Nuclear Magnetic Resonance Approaches in the Study of 2-Oxo Acid Dehydrogenase Multienzyme Complexes—A Literature Review [PDF]
Molecules, 2013 The 2-oxoacid dehydrogenase complexes (ODHc) consist of multiple copies of three enzyme components: E1, a 2-oxoacid decarboxylase; E2, dihydrolipoyl acyl-transferase; and E3, dihydrolipoyl dehydrogenase, that together catalyze the oxidative ...
Mulchand S. Patel +2 more
doaj +2 more sources
PLoS ONE, 2014 Multienzyme complexes catalyze important metabolic reactions in many organisms, but little is known about the complexes involved in biological methane production (methanogenesis). A crosslinking-mass spectrometry (XL-MS) strategy was employed to identify
Dillon J Lieber +6 more
doaj +2 more sources
Nature-inspired confined cascade enzyme nanoreactors for targeted atherosclerosis therapy [PDF]
Signal Transduction and Targeted TherapyOxidative stress and excessive inflammatory responses are major drivers of atherosclerosis (AS) formation and progression. In this study, we report a nature-inspired nanoreactor (named USPB@SeDMSN@NM) with superoxide dismutase (SOD), catalase (CAT), and ...
Yuehuang Wu +7 more
doaj +2 more sources
Application of artificial scaffold systems in microbial metabolic engineering
Frontiers in Bioengineering and Biotechnology, 2023 In nature, metabolic pathways are often organized into complex structures such as multienzyme complexes, enzyme molecular scaffolds, or reaction microcompartments. These structures help facilitate multi-step metabolic reactions.
Nana Liu +6 more
doaj +1 more source
Photosynthetic Electron Transfer by Dint of Protein Mobile Carriers. Multi-particle Brownian and Molecular Modeling [PDF]
EPJ Web of Conferences, 2019 The paper presents the review of works on modeling the interaction of photosynthetic proteins using the multiparticle Brownian dynamics method developed at the Department of Biophysics, Biological Faculty, Lomonosov Moscow State University.
Riznichenk Galina +4 more
doaj +1 more source
Multienzyme Coimmobilization on Triheterofunctional Supports [PDF]
, 2023 Immobilized multienzyme systems are gaining momentum in applied biocatalysis; however, the coimmobilization of several enzymes on one carrier is still challenging.
Susana Velasco-Lozano (1810726) +11 more
core +1 more source
Biomolecules, 2021 The use of multienzyme complexes can facilitate biocatalytic cascade reactions by employing fusion enzymes or protein tags. In this study, we explored the use of recently developed peptide tags that promote complex formation of the targeted proteins: the
Ni Nyoman Purwani +3 more
doaj +1 more source
Structure of the murine lysosomal multienzyme complex core [PDF]
Science Advances, 2021 The 3D structure of the complex formed by the enzymes GLB1 and CTSA furthers our understanding of lysosomal storage diseases.
Alexei Gorelik +4 more
openaire +2 more sources