Rapid multi-locus sequence typing using microfluidic biochips. [PDF]
Multiple locus sequence typing (MLST) has become a central genotyping strategy for analysis of bacterial populations. The scheme involves de novo sequencing of 6-8 housekeeping loci to assign unique sequence types. In this work we adapted MLST to a rapid
Timothy D Read +7 more
doaj +1 more source
Multilocus Sequence Typing [PDF]
Multilocus sequence typing (MLST) was first proposed in 1998 as a typing approach that enables the unambiguous characterization of bacterial isolates in a standardized, reproducible, and portable manner using the human pathogen Neisseria meningitidis as the exemplar organism.
Ana Belén, Ibarz Pavón +1 more
openaire +2 more sources
Multilocus sequence typing of IncN plasmids [PDF]
Incompatibility group N (IncN) plasmids have been associated with the dissemination of antimicrobial resistance and are a major vehicle for the spread of bla(VIM-1) in humans and bla(CTX-M-1) in animals. A plasmid multilocus sequence typing (pMLST) scheme was developed for rapid categorization of IncN plasmids.Twelve fully sequenced IncN plasmids ...
García-Fernández A +6 more
openaire +3 more sources
Evaluation of WGS-subtyping methods for epidemiological surveillance of foodborne salmonellosis [PDF]
Background: Salmonellosis is one of the most common foodborne diseases worldwide. Although human infection by non-typhoidal Salmonella (NTS) enterica subspecies enterica is associated primarily with a self-limiting diarrhoeal illness, invasive bacterial ...
Mohammed, M. +3 more
core +1 more source
FastMLST: A Multi-core Tool for Multilocus Sequence Typing of Draft Genome Assemblies
Multilocus Sequence Typing (MLST) is a precise microbial typing approach at the intra-species level for epidemiologic and evolutionary purposes. It operates by assigning a sequence type (ST) identifier to each specimen, based on a combination of alleles ...
E. Guerrero-Araya +3 more
semanticscholar +1 more source
The environmental bacterium Pseudomonas aeruginosa, particularly multidrug-resistant clones, is often associated with nosocomial infections and outbreaks.
Hauke Tönnies +3 more
semanticscholar +1 more source
Background Infections caused by Corynebacterium ulcerans, a zoonotic pathogen, have been reported worldwide. This microorganism is known to produce the diphtheria toxin and cause diphtheria‐like illness.
Koji Wake +10 more
doaj +1 more source
Novel multilocus sequence typing scheme reveals high genetic diversity of human pathogenic members of the Fusarium incarnatum-F. equiseti and F. chlamydosporum species complexes within the United States [PDF]
Species limits within the clinically important Fusarium incarnatum-F. equiseti and F. chlamydosporum species complexes (FIESC and FCSC, respectively) were investigated using multilocus DNA sequence data.
O'Donnell, K. +5 more
core +1 more source
Lack of continuity between Cronobacter biotypes and species as determined using multilocus sequence typing [PDF]
The accuracy of the Cronobacter biotyping scheme was compared with the 7-loci multilocus sequence typing scheme. Biotyping did not reliably assign species level identification, as only half (17/31) of the biotype variants were unique to any of the seven ...
Baldwin +19 more
core +1 more source
Molecular typing of the pneumococcus and its application in epidemiology in sub-Saharan Africa
Molecular typing of the pneumococcus has played a crucial role in understanding the epidemiology of the organism. However, most of what is known about molecular epidemiology of the pneumococcus pertains to the developed world.
Eric S Donkor
doaj +1 more source

