Results 21 to 30 of about 27,933 (201)

Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria [PDF]

Journal of Clinical Microbiology, 2012
ABSTRACT Accurate strain identification is essential for anyone working with bacteria. For many species, multilocus sequence typing (MLST) is considered the “gold standard” of typing, but it is traditionally performed in an expensive and time-consuming manner.
Larsen, Mette V, Cosentino, Salvatore, Rasmussen, Simon, Friis, Carsten, Hasman, Henrik, Marvig, Rasmus Lykke, Jelsbak, Lars, Sicheritz-Pontén, Thomas, Ussery, David W, Aarestrup, Frank M, Lund, Ole   +10 more
openaire   +3 more sources

Molecular typing of the pneumococcus and its application in epidemiology in sub-Saharan Africa

Frontiers in Cellular and Infection Microbiology, 2013
Molecular typing of the pneumococcus has played a crucial role in understanding the epidemiology of the organism. However, most of what is known about molecular epidemiology of the pneumococcus pertains to the developed world.
Eric S Donkor
doaj   +1 more source

Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans

Emerging Infectious Diseases, 2020
We conducted molecular typing of a Corynebacterium ulcerans isolate from a woman who died in Japan in 2016. Genomic DNA modification might have affected the isolate’s ribotyping profile.
Tsuyoshi Sekizuka, Chihiro Katsukawa, Makoto Kuroda, Keigo Shibayama, Ken Otsuji, Mitsumasa Saito, Akihiko Yamamoto, Masaaki Iwaki   +7 more
doaj   +1 more source

High-throughput multilocus sequence typing: bringing molecular typing to the next level. [PDF]

PLoS ONE, 2012
Multilocus sequence typing (MLST) is a widely used system for typing microorganisms by sequence analysis of housekeeping genes. The main advantage of MLST in comparison to other typing techniques is the unambiguity and transferability of sequence data ...
Stefan A Boers, Wil A van der Reijden, Ruud Jansen   +2 more
doaj   +1 more source

Extended Sequence Typing of Campylobacter spp., United Kingdom

Emerging Infectious Diseases, 2008
Supplementing Campylobacter spp. multilocus sequence typing with nucleotide sequence typing of 3 antigen genes increased the discriminatory index achieved from 0.975 to 0.992 among 620 clinical isolates from Oxfordshire, United Kingdom.
Kate E. Dingle, Noel D. McCarthy, Alison J. Cody, Tim E.A. Peto, Martin C. J. Maiden   +4 more
doaj   +1 more source

Multilocus Sequence Typing of Clostridium difficile [PDF]

Journal of Clinical Microbiology, 2010
ABSTRACT A robust high-throughput multilocus sequence typing (MLST) scheme for Clostridium difficile was developed and validated using a diverse collection of 50 reference isolates representing 45 different PCR ribotypes and 102 isolates from recent clinical samples.
Griffiths, D, Fawley, W, Kachrimanidou, M, Bowden, R, Crook, D, Fung, R, Golubchik, T, Harding, R, Jeffery, K, Jolley, K, Kirton, R, Peto, T, Rees, G, Stoesser, N, Vaughan, A, Walker, A, Young, B, Wilcox, M, Dingle, K   +18 more
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Ovine clone ST1464: A predominant genotype of Staphylococcus aureus subsp. anaerobius isolated from sheep in Sudan

Journal of Infection in Developing Countries, 2010
Background: The aim of the present study was to examine the phenotypic and genotypic relatedness of 17 Staphylococcus aureus subsp. anaerobius isolates recovered from sheep abscesses in Khartoum state, Sudan, during the period 2007-2008.
Haitham Elbir, Edward J. Feil, Michel Drancourt, Véronique Roux, Sulieman M El Sanousi, Mona Eshag, Patricia Colque-Navarro, Inger Kühn, Jan-Ingmar Flock   +8 more
doaj   +1 more source

mlstdbNet – distributed multi-locus sequence typing (MLST) databases

BMC Bioinformatics, 2004
Background Multi-locus sequence typing (MLST) is a method of typing that facilitates the discrimination of microbial isolates by comparing the sequences of housekeeping gene fragments.
Maiden Martin CJ, Chan Man-Suen, Jolley Keith A   +2 more
doaj   +1 more source

Expanded Multilocus Sequence Typing for Burkholderia Species [PDF]

Journal of Clinical Microbiology, 2009
ABSTRACT PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B . cepacia complex species, (ii) amplify these same loci from additional
Spilker, T., Baldwin, A., Bumford, A., Dowson, C. G, Mahenthiralingam, Eshwar, LiPuma, J. J.   +5 more
openaire   +2 more sources

Consensus Multilocus Sequence Typing Scheme for Pneumocystis jirovecii

Journal of Fungi, 2020
Pneumocystis jirovecii is an opportunistic human pathogenic fungus causing severe pneumonia mainly in immunocompromised hosts. Multilocus sequence typing (MLST) remains the gold standard for genotyping of this unculturable fungus.
Lana Pasic, Lidia Goterris, Mercedes Guerrero-Murillo, Laszlo Irinyi, Alex Kan, Carolina A. Ponce, Sergio L. Vargas, M. Teresa Martin-Gomez, Wieland Meyer   +8 more
doaj   +1 more source