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Multiphoton microscopy in dermatological imaging
A minimally invasive imaging modality that provides both cellular and extracellular structural information with subcellular resolution is helpful for clinical diagnosis as well as basic laboratory research in dermatology. Multiphoton microscopy (MPM), using femtosecond laser as the light source, is efficient in non-linear excitation of endogenous ...
Tsung-Hua, Tsai +3 more
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Multiphoton microscopy in biological research
Current Opinion in Chemical Biology, 2001From its conception a decade ago, multiphoton microscopy has evolved from a photonic novelty to an indispensable tool for gleaning information from subcellular events within organized tissue environments. Its relatively deep optical penetration has recently been exploited for subcellularly resolved investigations of disease models in living transgenic ...
Rebecca M Williams +2 more
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New developments in multiphoton microscopy
Multiphoton laser-scanning microscopy is still developing rapidly, both technologically and by broadening its range of application. Technical progress has been made in the optimization of fluorophores, in increasing the imaging depth of multiphoton microscopy, and in microscope miniaturization.
Helmchen, F., Denk, W.
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Multiphoton Microscopy Applications in Biology
This chapter provides an overview of basic principles of multiphoton microscopy, including a brief summary of how multiphoton microscopy fits within the arena of fluorescent microscopy techniques. The description of how multiphoton microscopy works is geared toward both the novice and experienced user.
Yamada, Miko +2 more
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Multiphoton microscopy in life sciences
Journal of Microscopy, 2000Near infrared (NIR) multiphoton microscopy is becoming a novel optical tool of choice for fluorescence imaging with high spatial and temporal resolution, diagnostics, photochemistry and nanoprocessing within living cells and tissues. Three‐dimensional fluorescence imaging based on non‐resonant two‐photon or three‐photon fluorophor excitation requires ...
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Principles of Multiphoton Microscopy
Nephron Experimental Nephrology, 2006Multiphoton fluorescence microscopy is a powerful, important tool in biomedical research that offers low photon toxicity and higher spatial and temporal resolution than other in vivo imaging modalities. The capability to collect images hundreds of micrometers into biological tissues provides an invaluable tool for studying cellular and subcellular ...
Kenneth W, Dunn, Pamela A, Young
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2006
The proceedings contain 44 papers. The topics discussed include: time-resolved nanoscale imaging of biomolecules in living cells and tissues: prospects for small-animal imaging; spectrally resolved fluorescence lifetime and FRET measurements; three-dimensional FRET microscopy; improving fluorescence diagnosis of cancer by SLIM; scanning total internal ...
DIASPRO, ALBERTO GIOVANNI +4 more
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The proceedings contain 44 papers. The topics discussed include: time-resolved nanoscale imaging of biomolecules in living cells and tissues: prospects for small-animal imaging; spectrally resolved fluorescence lifetime and FRET measurements; three-dimensional FRET microscopy; improving fluorescence diagnosis of cancer by SLIM; scanning total internal ...
DIASPRO, ALBERTO GIOVANNI +4 more
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Multiphoton microscopy applications in nanodermatology
WIREs Nanomedicine and Nanobiotechnology, 2012AbstractMultiphoton microscopy is being used as a tool for tracking nanoparticles and assessing endogenous autofluorescent molecules. This technology is founded on femtosecond lasers that are capable of exciting unlabeled metal nanoparticles, quantum dots, and upconverting nanoparticles.
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Multimodal multiphoton microscopy
SPIE Proceedings, 2009Multiphoton microscopy is a powerful technique for high spatial resolution thick tissue imaging. In its simple version, it uses a high repetition rate femtosecond oscillator laser source focussed and scanned across biological sample that contains fluorophores.
François Légaré +2 more
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