Results 131 to 140 of about 50,764 (304)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

A numerical Green’s function for multiple cracks in anisotropic bodies

, 2020
. The numerical construction of a Green's function for multiple interacting planar cracks in an anisotropic elastic space is considered. The numerical Green's function can be used to obtain a special boundary integral method for an important ...
J C F Telles, W T Ang
core  

Soil desiccation cracking triggered by surface defects: Insight and mechanism based on strain/displacement analysis using DIC

Journal of Rock Mechanics and Geotechnical Engineering
Soil desiccation cracking is a prevalent natural phenomenon that poses significant geotechnical and geoenvironmental challenges. Cracks typically initiate at surface defects such as air bubbles, large aggregates, tiny pits, or uneven surfaces, where ...
Tao Wang, Chao-Sheng Tang, Luan Lin, Zhixiong Zeng, Qing Cheng, Zhengtao Shen   +5 more
doaj   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Stress Intensity Factors of Double and Multiple Edge Cracks

, 1996
In this paper the interaction of multiple edge cracks in a semi-infinite plate is considered. The problem is formulated as a system of hypersingular integral equations using the stress field due to a force doublet as a fundamental solution.
野田, 尚昭, 鶴, 元樹, 小田, 和広   +2 more
core  

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

A micromechanical damage theory for brittle materials with small cracks

, 1998
For brittle solids containing numerous small cracks, a micromechanical damage theory is presented which accounts for the interactions between different small cracks and the effect of the boundary of a finite solid, and includes growth of the pre-existing
王自强, 韩学礼, Zhan, S (reprint author), Chinese Acad Sci, Inst Mech, LNM, Beijing 100080, Peoples R China., 詹世革   +3 more
core  

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Multiple Crack Weight for Solution of Multiple Interacting Cracks by Meshless Numerical Methods

, 2008
We devise a multiple crack weight (MCW) method for the accurate and effective solution of strongly interacting cracks by meshless numerical methods. The MCW method constructs weight functions around cracks so that they simultaneously characterize all the
Eli Turkel, Boris Muravin
core