Results 131 to 140 of about 1,719,427 (359)

Diagnostic Performance of a Multiplex PCR assay for meningitis in an HIV-infected population in Uganda

Diagnostic microbiology and infectious disease, 2015
Meningitis remains a worldwide problem, and rapid diagnosis is essential to optimize survival. We evaluated the utility of a multiplex PCR test in differentiating possible etiologies of meningitis.
Joshua Rhein, N. Bahr, A. Hemmert, J. Cloud, Satya K. Bellamkonda, C. Oswald, Eric Lo, Henry W. Nabeta, R. Kiggundu, A. Akampurira, A. Musubire, Darlisha A Williams, D. Meya, D. Boulware   +13 more
semanticscholar   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

Evaluating an MRNA based body fluid identification test using sybr green fluorescent dye and real-time PCR

, 2011
The requirement to have more definitive and wider ranging body fluid identification (BFI) tests has resulted in a range of mRNA based real-time PCR BFI assays utilising Taqman fluorescent dye.
Connolly, Jo-Ann, Williams, Graham
core  

Multiple Colonization with S. pneumoniae before and after Introduction of the Seven-Valent Conjugated Pneumococcal Polysaccharide Vaccine [PDF]

, 2010
Background: Simultaneous carriage of more than one strain of Streptococcus pneumoniae promotes horizontal gene transfer events and may lead to capsule switch and acquisition of antibiotic resistance. We studied the epidemiology of cocolonization with S.
Hinds Jason, Brugger, Silvio D., Frey, Pascal, Hinds, Jason, Brugger Silvio D., Frey Pascal, Silvio D Brugger, Silvio D. Brugger, Hinds, J, Brugger, SD, Aebi, S, Jason Hinds, Kathrin Mühlemann, Mühlemann, K, Suzanne Aebi, Mühlemann Kathrin, Aebi Suzanne, Aebi, Suzanne, Mühlemann, Kathrin, Pascal Frey, Frey, P   +20 more
core   +1 more source

DOT1L Drives Endothelial‐to‐Mesenchymal Transition and Fibrotic Vascular Remodeling via H3K79 Methylation

Advanced Science, EarlyView.
DOT1L as a central epigenetic regulator of EndoMT and pulmonary fibrosis. Acting as an early epigenetic switch, it translates TGFβ–SMAD signaling into H3K79me2‐mediated chromatin remodeling, selectively activates fibrosis‐related genes, and primes ECs for rapid mesenchymal transition.
Yaofeng Wang, Xing Peng, Jingjing Chen, Yun Zhang, Tinghong Zhang, Jingyuan Zhang, Jiaying Fan, Hui Zheng, Qiaoyuan Liu, Zhimin Song, Zhan‐Peng Huang, Shu Meng   +11 more
wiley   +1 more source

Mouse multiplex PCR assay.

, 2019
Mouse multiplex PCR assay.
Jamie L. Almeida (3212301), Karin Kindig (6862052), Ruth Peat (6862064), Heath Berg (6862076), Barbara B. Fransway (6862085), Aleksandra Dakic (399057), Carolyn R. Steffen (3684418), Yvonne Reid (6862097), Beth Bauer (6862091), Maikan Kone (6862055), Jayamalini Holding-Pillai (6862067), Steve Lund (6862082), Manuela Natoli (132738), Erin M. Hall (6862070), Jinliang Li (75116), Dale W. Lewis (6862094), Deborah L. D. Letham (6862058), Scott Langdon (6862061), Susanne M. Gollin (5063618), Georges Wigger (6862079), Mark Ladd (664581), Bob Geraghty (6862088), Megan D. Shaffer (6862073)   +22 more
core   +1 more source

Multiplex Detection of Aspergillus fumigatus Mycoviruses

Viruses, 2018
Mycoviruses are viruses that naturally infect and replicate in fungi. They are widespread in all major fungal groups including plant and animal pathogenic fungi. Several dsRNA mycoviruses have been reported in Aspergillus fumigatus.
Selin Özkan-Kotiloğlu, Robert H. A. Coutts   +1 more
doaj   +1 more source

New multiplex PCR methods for rapid screening of genetically modified organisms in foods

Frontiers in Microbiology, 2015
We present novel multiplex PCR methods for rapid and reliable screening of genetically modified organisms (GMOs). New designed PCR primers targeting four frequently used GMO specific sequences permitted identification of new DNA markers, in particular ...
N. Datukishvili, T. Kutateladze, I. Gabriadze, Kakha Bitskinashvili, B. Vishnepolsky   +4 more
semanticscholar   +1 more source

Targeting Peptostreptococcus anaerobius with an Iron‐Based Nanozyme Reverses Ferroptosis Resistance and Enhances Antitumor Immunity in Colorectal Cancer

Advanced Science, EarlyView.
An iron‐based nanozyme selectively eliminates intratumoral P. anaerobius while catalytically generating ROS to induce ferroptosis, synergistically suppressing colorectal cancer growth and activating anti‐tumor immunity through immunogenic cell death. ABSTRACT The intratumoral microbiota is a critical determinant of therapeutic outcomes in colorectal ...
Yinghao Cao, Jun Wang, Hanwenchen Wang, Xiang Sun, Fang Fang, Shiying Li, Jianping Liu, Changrong Shi, Pengyuan Qi, Jianhua Zou, Xiaoyuan Chen, Kailin Cai   +11 more
wiley   +1 more source

Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping-2

, 2011
Barc64, (C) barc108, (D) gwm681, (E) barc273 and (F) cfa2028, and 100, 70, 120, 70, 120 and 30 nM of locus-specific primer, respectively.Copyright information:Taken from "Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping"http://www.
Matthew J Hayden (44210), Kenneth J Chalmers (44213), Thao M Nguyen (44211), Amanda Waterman (44212)   +3 more
core   +1 more source