Results 111 to 120 of about 1,719,427 (359)

Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping

BMC Genomics, 2008
Background Microsatellite (SSR) and single nucleotide polymorphism (SNP) markers are widely used in plant breeding and genomic research. Thus, methods to improve the speed and efficiency of SSR and SNP genotyping are highly desirable.
Nguyen Thao M, Hayden Matthew J, Waterman Amanda, Chalmers Kenneth J   +3 more
doaj   +1 more source

Improved detection of Escherichia coli and coliform bacteria by multiplex PCR

BMC Biotechnology, 2015
BackgroundThe presence of coliform bacteria is routinely assessed to establish the microbiological safety of water supplies and raw or processed foods.
F. Molina, Elena López-Acedo, R. Tabla, I. Roa, A. Gómez, J. Rebollo   +5 more
semanticscholar   +1 more source

LMO7 Suppresses Tumor‐Associated Macrophage Phagocytosis of Tumor Cells Through Degradation of LRP1

Advanced Science, EarlyView.
LMO7 in tumor‐associated macrophages suppresses phagocytosis of tumor cells and limits cytotoxic T lymphocytes infiltration, fostering tumor progression. Mechanistically, LMO7 mediates the ubiquitination and degradation of the phagocytic receptor LRP1, impairing its ability to engulf tumor cells and driving macrophages toward an antitumor phenotype ...
Mengkai Li, Tao Wang, Yuwen Zhong, Zhiming Wang, Wei Li, Zejian Wang, Xinyi Yang, Yu Qiao, Jianfeng Xiang, Wei Gu, Zishu Wang, Lei Sun, Feng Qian   +12 more
wiley   +1 more source

Multiplex PCR alkalmazása gazdasági állatfajok azonosításában

, 2015
Napjainkban egyre nagyobb figyelmet kap a különböző állati eredetű hús- illetve tejtermékek kapcsán a gazdasági haszonállatok fajazonosítása. Erre a közelmúlt élelmiszerhamisítás botrányai is felhívják a figyelmet.
Katona, Frida
core  

Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR

Indonesian Journal of Biotechnology, 2006
world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detecting dengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primers that conserve for several ...
Nastiti Wijayanti, Hera Nirwati, Tri Wibawa, Aris Haryanto, S. Sutaryo   +4 more
doaj   +1 more source

A Single-Tube, Functional Marker-Based Multiplex PCR Assay for Simultaneous Detection of Major Bacterial Blight Resistance Genes Xa21, xa13 and xa5 in Rice ☆

, 2016
In marker-assisted breeding for bacterial blight (BB) resistance in rice, three major resistance genes, viz., Xa21 , xa13 and xa5 , are routinely deployed either singly or in combinations.
S. Hajira, R. Sundaram, G. S. Laha, A. Yugander, S. Balachandran, B. C. Viraktamath, K. Sujatha, C. Balachiranjeevi, K. Pranathi, M. Anila, S. Bhaskar, V. Abhilash, H. Mahadevaswamy, M. Kousik, T. Kumar, G. Harika, G. Rekha   +16 more
semanticscholar   +1 more source

Multiplex PCR Tests for Detection of Pathogens Associated with Gastroenteritis

Clinics in Laboratory Medicine, 2015
Synopsis A wide range of enteric pathogens can cause infectious gastroenteritis. Conventional diagnostic algorithms including culture, biochemical identification, immunoassay and microscopic examination are time consuming and often lack sensitivity and ...
Hongwei Zhang, S. Morrison, Yi-Wei Tang
semanticscholar   +1 more source

CDK4/6 Inhibition Induces CD8+ T Cell Antitumor Immunity via MIF‐Induced Functional Orchestration of Tumor‐Associated Macrophages

Advanced Science, EarlyView.
CDK4/6 inhibition promotes CD8+ T cell expansion through tumor‐macrophage crosstalk by activating HIF‐1α and enhancing MIF‐CD44/CD74 signaling. This reprograms TAMs to boost MHC‐I antigen presentation, and CDK4/6 inhibitor‐trained M1 TAM supernatant therapy synergizes with low‐dose PD‐1 blockade to restore antitumor immunity.
Lin He, Yuzhong Peng, Lat‐lun Leong, Jingbo Zhou, Dongyang Tang, Weilu Wang, Xiaoran Wu, Josh haipeng Lei, Yongqin Ye, Yangyang Feng, Yunfeng Qiao, Xiangpeng Chu, Di Mu, Qi Zhao, Tzuming Liu, Yan Chen, Paul Kwonghang Tam, Chu‐Xia Deng   +17 more
wiley   +1 more source

Robust detection and identification of multiple oomycetes and fungi in environmental samples by using a novel cleavable padlock probe-based ligation-detection assay

, 2009
Simultaneous detection and identification of multiple pathogenic microorganisms in complex environmental samples are required in numerous diagnostic fields.
Dullemans, A.M., Slawiak, M., Dullemans, A., Van Doorn, R., Doorn, R., van, Kowalchuk, G.A., Doorn, R. van, Schoen, C.D., Szemes, M., Bonants, P.J.M., Bonants, P.   +10 more
core   +1 more source

GATA4‐Driven Transcription of HtrA1 Promotes Cellular Senescence in Ménière's Disease and Age‐Related Audio‐Vestibular Dysfunction

Advanced Science, EarlyView.
This study identifies the HDAC6/GATA4/HtrA1 axis as a critical driver of cellular senescence in the inner ear. GATA4 nuclear translocation, facilitated by HDAC6 downregulation, transcriptionally activates HtrA1, promoting hair cell senescence, SASP, and audio‐vestibular dysfunction in models of Ménière's disease and age‐related audio‐vestibular ...
Na Zhang, Na Li, Yan Wang, Jing Zhang, Jiahui Liu, Lei Chen, Yongdong Song, Yurong Mu, Yuechen Han, Yafeng Lyu, Xiaofei Li, Hanyue Wang, Jing Wang, Yao Lu, Zhaomin Fan, Daogong Zhang, Haibo Wang   +16 more
wiley   +1 more source