Results 101 to 110 of about 1,719,427 (359)

Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs

Journal of Veterinary Medical Science, 2016
Canine infectious respiratory disease complex (CIRDC) viruses have been detected in dogs with respiratory illness. Canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine distemper virus (CDV), canine respiratory coronavirus (CRCoV ...
Chutchai Piewbang, A. Rungsipipat, Y. Poovorawan, S. Techangamsuwan   +3 more
semanticscholar   +1 more source

PD‐1 Inhibits CD4+ TRM‐Mediated cDC1 Mobilization via Suppressing JAML in Human NSCLC

Advanced Science, EarlyView.
CD4+ tissue‐resident memory T cells (TRMs) in non‐small cell lung cancer recruit conventional type 1 dendritic cells via XCL1‐XCR1 signaling, orchestrating antitumor immunity. The costimulatory molecule JAML is essential for this process. PD‐1 blockade restores JAML expression and cDC1 mobilization, while JAML agonists synergize with anti‐PD‐1 therapy,
Zheyu Shao, Qinyuan Liu, Zhongwei Xin, Zhiyao Zhou, Mingjie Lin, Di Chen, Zhixing Hao, Yongyuan Chen, Wenxuan Wu, Shuyang Zhang, Xiaoke Chen, Xia Xu, Jinfan Li, Wei Lin, Yuhao Lu, Dang Wu, Pin Wu   +16 more
wiley   +1 more source

Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping-4

, 2011
Ex-ready PCR assays were performed using 80, 70, 40 and 40 nM of locus-specific primer, respectively. Conventional PCR was performed with T55, 60, 60 and 60°C, respectively.
Matthew J Hayden (44210), Kenneth J Chalmers (44213), Thao M Nguyen (44211), Amanda Waterman (44212)   +3 more
core   +1 more source

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205), Ameet J. Pinto (143202), Lutgarde Raskin, Pinto Ameet J., Raskin, L., Ameet J Pinto, Raskin Lutgarde, Pinto, A.   +7 more
core   +1 more source

Identification of respiratory virus in infants with congenital heart disease by comparison of different methods

Revista do Instituto de Medicina Tropical de São Paulo, 2011
Respiratory virus infections are the main cause of infant hospitalization and are potentially severe in children with congenital heart disease (CHD).
Tatiana Mitiko Kanashiro, Lucy Santos Vilas Boas, Ana Maria Thomaz, Tania Regina Tozetto-Mendoza, Mônica Setsuko, Clarisse Martins Machado   +5 more
doaj   +1 more source

Multiplex PCR System for the Diagnosis of Plague

Current Molecular Medicine
The plague caused by Yersinia pestis has a high case fatality rate. It is often transmitted from person to person through mosquito bites, causing serious disease transmission. Due to its clinical symptoms being very similar to influenza, it is difficult to detect by people. Traditional detection methods for Y.
Wang, Wenfang, Zhang, Xiaoxue, Yu, Hui, Wang, Zhanli   +3 more
openaire   +2 more sources

Multiplex PCR for rapid diagnosis of gastrointestinal tuberculosis

Journal of Global Infectious Diseases, 2013
Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance.To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB.MPCR was performed on colonoscopy biopsy specimens on 11 GITB confirmed (culture/AFB/histopathology was positive), 29 GITB suspected and 30 ...
Kusum Sharma, Saroj Kant Sinha, Aman Sharma, Ritambra Nada, Kaushal K Prasad, Kapil Goyal, Surinder Singh Rana, Deepak Kumar Bhasin, Meera Sharma   +8 more
openaire   +3 more sources

Targeting UXS1‐Dependent Glucuronate Detoxification Potentiates Metformin's Anti‐Tumor Efficacy in Lung Adenocarcinoma

Advanced Science, EarlyView.
This study reveals that metformin promotes glucuronic acid metabolism in lung adenocarcinoma by activating UGDH S476 phosphorylation and enhancing the conversion of UDPG to UDPGA based on metabolomics analysis. Through compound virtual screening, it is found that plantainoside targeting UGDH downstream UXS1 leads to UDPGA toxicity accumulation ...
Qihai Sui, Zhencong Chen, Guangyao Shan, Zhengyang Hu, Xing Jin, Jiaqi Liang, Yanjun Yi, Jiacheng Yin, Haochun Shi, Xifei Jiang, Junjie Xi, Zongwu Lin, Cheng Zhan, Fenghao Sun, Wei Jiang   +14 more
wiley   +1 more source

Development of a Multiplex PCR to Identify Lactobacillus acidophilus NCFM, a Probiotic Organism

, 2010
Lactobacillus acidophilus NCFM is a probiotic organism used in food products and dietary supplements since 1972. A recent study recovered strains of L.
Souther, Nicole Elaine
core  

Clonagem, expressão e purificação de lipase de Staphylococcus xylosus e detecção de genes de enterotoxinas de Staphylococcus aureus isolados de produtos de origem animal [PDF]

, 2008
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-graduação em Ciência dos AlimentosStaphylococcus xylosus é um microrganismo envolvido na fermentação de produtos cárneos e produtor de lipases ...
Pelisser, Marcia Regina
core