Results 81 to 90 of about 1,719,427 (359)

Analysis of multiplex PCR fragments with PMMA microchip

, 2006
Microchip electrophoresis is a promising technique for analysis of bio-molecules. It has the advantages of fast analysis, high sensitivity, high resolution and low-cost of samples.
Zhong, RT, Xiong, W, 林炳承, Ye, NN, Mei, XD, Liu, DY, Zhou, XM, Chang, GH, Lin, BC   +8 more
core   +1 more source

Simplified development of multiplex real-time PCR through master mix augmented by universal fluorogenic reporters

BioTechniques, 2016
Mediator probe (MP) PCR is a real-time PCR approach that uses standardized universal fluorogenic reporter oligonucleotides (UR) in conjunction with label-free sequence-specific probes.
Simon Wadle, Michael Lehnert, Friedrich Schuler, René Köppel, Annerose Serr, Roland Zengerle, Felix von Stetten   +6 more
doaj   +1 more source

A Fully Human Engineered Bone Niche With Endogenous Osteoclastogenesis Reveals Osteoclast‐Dependent Osteomimicry in Prostate Cancer Cells

Advanced Healthcare Materials, EarlyView.
A human 3D bone niche integrating osteoblasts and osteoclasts enables co‐culture with prostate cancer cell lines and patient‐derived organoids. The engineered niche (i) models aspects of reciprocal phenotypic regulation between osteoblasts and cancer cells, (ii) captures osteoclast‐enhanced osteomimicry in tumor cells, and (iii) establishes a ...
Andrea Mazzoleni, Robin Dolgos, Thomas Menter, Boris Dasen, Arnaud Scherberich, Clémentine Le Magnen, Manuele G. Muraro, Ivan Martin   +7 more
wiley   +1 more source

Comparison of Two Multiplex PCR Systems for Meat Species Authentication

Journal of Food Quality and Hazards Control, 2019
Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication.
D. Al-taghlubee, A. Misaghi, P. Shayan, A. Akhondzadeh Basti, H. Gandomi, D. Shayan   +5 more
doaj  

3D‐Printed Piezoionic/Bioelectronic Hydrogel for Electro‐Metabolic Regulation of Osteogenic Differentiation

Advanced Healthcare Materials, EarlyView.
A 3D‐printed piezoionic GPMx hydrogel enables stable electromechanical signal generation under mechanical loading, exhibiting long‐term durability and low fatigue. As a bioactive patch, it restores endogenous bioelectricity to stimulate osteogenesis via Ca2+ influx and mitochondrial activation, while simultaneously enabling label‐free alkaline ...
Sayan Deb Dutta, Myoung Joon Jeon, Youjin Seol, Ki‐Taek Lim   +3 more
wiley   +1 more source

In-situ rapid bioaerosol detection in the ambient air by miniature multiplex PCR utilizing technique

, 2021
Given the current problems associated with the COVID-19 pandemic, research in the field of rapid and reliable detection and characterization of airborne pathogenic microorganisms is becoming of exceptional urgency.
Usachev, EV, Agranovski, IE
core   +1 more source

A Multiplex RT-PCR Assay for S. aureus, L. monocytogenes, and Salmonella spp. Detection in Raw Milk with Pre-enrichment

Frontiers in Microbiology, 2017
This study firstly developed a multiplex real-time PCR (RT-PCR) technique combined with a pre-enrichment step to simultaneously detect Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes) and Salmonella spp.
Tian Ding, Yuanjie Suo, Zhaohuan Zhang, Donghong Liu, Xingqian Ye, Shiguo Chen, Yong Zhao   +6 more
doaj   +1 more source

Hierarchically Engineered Multi‐Enzyme Nanoreactors for in vitro Drug Biosynthesis and Pathway Transplantation Into Cells

Advanced Materials, EarlyView.
A hierarchically structured metal–organic framework (eMIL) is shown to host the complete six‐enzyme biosynthesis pathway for the anti‐cancer pigment violacein, reshaping reaction dynamics and multiplying in vitro yields. eMIL nanoreactors deliver this reconstituted bacterial multi‐protein system into mammalian cells, where cell‐derived substrates and ...
Ainur Sharip, Somayah S. Qutub, Manar M. Farooqui, Walaa Baslyman, Nida Khalfay, Lukman O. Alimi, Patricia Lopez Sanchez, Lingyun Zhao, Milena Chernyshevskaia, Giovanni Colombo, Niveen M. Khashab, Stefan T. Arold, Raik Grünberg   +12 more
wiley   +1 more source

Sensitive and Spatially‐Resolved Electrochemiluminescence via Micropatterning

Advanced Materials Interfaces, EarlyView.
The use of a simple silica nanoparticle surface coating and patterning process can transform ordinary gold electrodes into multiplexed ECL sensing electrodes. Through spatially confined droplet formation and local ECL emission, it is possible to perform multiple analyses simultaneously from individual locations as well as intensify the ECL signal by as
Yuliang Shao, Tatiana Starikova, Mengzhen Xi, Maria Vittoria Balli, Emanuele Luigi Sciuto, Gabriele Giagu, Vincenzo Paratore, Carmelo Corsaro, Enza Fazio, Sabrina Conoci, Luca Prodi, Giovanni Valenti, Pavel A. Levkin   +12 more
wiley   +1 more source

A novel XbaI multiplex PCR method for rapid typing of Klebsiella pneumoniae strains

Scientific Reports
This study focused on exploiting single-nucleotide polymorphism (SNP) variations in and around XbaI-restriction sites (5′….T↓CTAGA….3′) within the genomes of Klebsiella pneumoniae to develop a multiplex PCR genotyping technique that integrates the high ...
Ibrahim Ali Al-Zahrani
doaj   +1 more source