Results 91 to 100 of about 957,735 (260)

Primers used for multiplex PCR.

open access: yes, 2021
Primers used for multiplex PCR.
Sirivipa Piyamongkol (11450269)   +4 more
core   +1 more source

Coixenolide Enhances Antitumor Immunity of Cytotoxic CD8+ T Cells by Inhibiting S100A8/A9‐CD36 Axis in Obesity‐Associated Pancreatic Adenocarcinoma

open access: yesAdvanced Science, EarlyView.
Pancreatic adenocarcinoma (PAAD) remains highly lethal with limited treatment options. This study demonstrates that coixenolide, a bioactive compound from Coix lacryma‐jobi L. and a key component of the clinically approved Kanglaite injection, exhibits enhanced antitumor efficacy in high‐fat diet (HFD)‐induced obese mice bearing PAAD tumors compared to
Kaidi Chen   +20 more
wiley   +1 more source

Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces [PDF]

open access: yes, 2003
A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and
Phillips, N.D., La, T., Hampson, D.J.
core  

Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping-4

open access: yes, 2011
Ex-ready PCR assays were performed using 80, 70, 40 and 40 nM of locus-specific primer, respectively. Conventional PCR was performed with T55, 60, 60 and 60°C, respectively.
Matthew J Hayden (44210)   +3 more
core   +1 more source

Compensatory Mitophagy via NEDD4/HIF‐1α/BNIP3 Pathway Restrains EndMT and Renal Allograft Interstitial Fibrosis Induced by TNFα

open access: yesAdvanced Science, EarlyView.
The role of TNFα in the process of renal allograft interstitial fibrosis is complex and multifaceted. As it can promote renal allograft interstitial fibrosis by inducing mitochondrial dysfunction and EndMT, and also mediating compensatory mitophagy through the NEDD4–HIF‐1α–BNIP3 pathway, which clears damaged mitochondria and inhibits EndMT, thereby ...
Dengyuan Feng   +15 more
wiley   +1 more source

Rab1A Promotes Hepatic Steatosis by Suppressing Mitophagy via the Raf‐1/ERK1/2/PINK1 Signaling Axis

open access: yesAdvanced Science, EarlyView.
Why does fat accumulate in the liver? Our study reveals Rab1A as the molecular switch that silences the cell's mitochondrial cleanup crew. Turning Rab1A off reactivates mitophagy, clears excess fat, and reverses fatty liver disease in mice—offering a promising new therapeutic target for MASLD, a growing global health challenge.
Li Zhang   +9 more
wiley   +1 more source

Evaluating an mRNA based body fluid identification test using SYBR green fluorescent dye and real-time PCR [PDF]

open access: yes
The requirement to have more definitive and wider ranging body fluid identification (BFID) tests has resulted in a range of mRNA based real-time PCR BFI assays utilising Taqman fluorescent dye.
Connolly, Jo-Ann
core   +3 more sources

PERFORMANCE OF MULTIPLEX REAL TIME PCR FOR DIAGNOSIS MALARIA IN FOREST ENDEMIC AREAS IN TANAH BUMBU REGENCY SOUTH KALIMANTAN PROVINCE INDONESIA [PDF]

open access: yes
and Figures Keywords: ABSTRACT performance-multiplex real time PCR-forest malaria Malaria incidence has declined in Indonesia for years, but it is still found a foci malaria in forest areas. Rapid Diagnostic Test (RDT) has been used as a diagnostic tool
Heny Arwati, Heny   +2 more
core   +1 more source

Detecção de Listeria monocytogenes em produtos a base de pescado e comparação de métodos PCR em tempo real e ISO11290-1 [PDF]

open access: yes, 2015
Dissertação (mestrrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Ciência dos Alimentos, Florianópolis, 2015.O pescado é um alimento de extrema importância na dieta dos seres humanos, pois possui ...
Barretta, Clarissa
core  

Copper‐Driven Epithelial Barrier Disruption: A Novel Mechanism of COPD Acute Exacerbations Mediated by the TNF‐α/ATP7A Axis

open access: yesAdvanced Science, EarlyView.
This study reveals a copper‐driven mechanism of COPD acute exacerbations mediated by the TNF‐α/ATP7A axis. TNF‐α suppresses the copper transporter ATP7A via competitive NF‐κB/CREB1/CBP signaling, resulting in intracellular copper accumulation, mitochondrial oxidative stress, and epithelial barrier disruption.
Xinru Xiao   +7 more
wiley   +1 more source

Home - About - Disclaimer - Privacy