Results 51 to 60 of about 877,269 (241)

Clinical Comparative Evaluation of the LabTurboTM AIO® Reverse Transcription-Polymerase Chain Reaction and World Health Organization-Recommended Assays for the Detection of Emerging SARS-CoV-2 Variants of Concern

Infection and Drug Resistance, 2022
Chih-Kai Chang,1,* Ming-Jr Jian,1,* Hsing-Yi Chung,1 Jung-Chung Lin,2 Shan-Shan Hsieh,1 Sheng‐Hui Tang,1 Cherng-Lih Perng,1 Chien-Wen Chen,3 Kuo-Sheng Hung,4 Feng-Yee Chang,2 Hung-Sheng Shang1 1Division of Clinical Pathology, Department of ...
Chang CK, Jian MJ, Chung HY, Lin JC, Hsieh SS, Tang S, Perng CL, Chen CW, Hung KS, Chang FY, Shang HS   +10 more
doaj  

qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples

STAR Protocols, 2020
Summary: The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents ...
Richard M. Giadone, Aditya Mithal, Gregory J. Miller, Taylor M. Matte, Anthony K. Yeung, Todd W. Dowrey, Rhiannon B. Werder, Nancy S. Miller, Christopher D. Andry, Kim Vanuytsel, George J. Murphy   +10 more
doaj   +1 more source

Bioprinted Stiffness‑Tunable Pancreatic Microniches to Decode Early Tumor Initiation Mechanisms

Advanced Healthcare Materials, EarlyView.
Stiffness‐tunable GelMA microniches generated by microvalve bioprinting preserve wild‐type acinar identity while revealing a nonlinear, Kras‐dependent response to matrix mechanics. Intermediate stiffness promotes acinar‐to‐ductal metaplasia and proliferation, defining a permissive mechanical window.
Aurelien Mazet, Charles Handschin, Theo Desigaux, Nathalie Dusserre, Marie‐Laure Stachowicz, Shane Fennell, Malou Lea, Silvia Arcucci, Benoît Thibault, Jean‐Christophe Fricain, Julie Guillermet‐Guibert, Hugo Oliveira   +11 more
wiley   +1 more source

Rab1A Promotes Hepatic Steatosis by Suppressing Mitophagy via the Raf‐1/ERK1/2/PINK1 Signaling Axis

Advanced Science, EarlyView.
Why does fat accumulate in the liver? Our study reveals Rab1A as the molecular switch that silences the cell's mitochondrial cleanup crew. Turning Rab1A off reactivates mitophagy, clears excess fat, and reverses fatty liver disease in mice—offering a promising new therapeutic target for MASLD, a growing global health challenge.
Li Zhang, Jianhua Li, Huilu Zhang, Qi Qin, Yichen Huang, Xiaofan Tian, Chao Sun, Binbin Li, Zhengxin Wang, Xin Zhang   +9 more
wiley   +1 more source

qRT-PCR primers.

, 2016
qRT-PCR primers.
Marc P. Stemmler (176442), Tim Pieters (3074160), Dagmar Wirth (48093), Vanessa Andries (74590), Danny Huylebroeck (124349), Kelly Lemeire (3074157), Jody J. Haigh (426911), Riet De Rycke (416524), Jolanda van Hengel (253211), Agata Stryjewska (469825), Frans van Roy (3074154), Lieven Haenebalcke (734219), Geert Berx (12850), Steven Goossens (426910), Tino Hochepied (493717)   +14 more
core   +1 more source

qRT-PCR assays for Jamestown Canyon virus quantification.

, 2023
qRT-PCR assays for Jamestown Canyon virus quantification.
Anastacia M. Diaz (16934310), Fhallon Ware-Gilmore (11172140), Elizabeth A. McGraw (7853180), Matthew J. Jones (555280), Heverton L. C. Dutra (6714785), Meng-Jia Lau (7827683), Brianna P. McNulty (16934307)   +6 more
core   +1 more source

Evaluation of a viral microarray based on simultaneous extraction and amplification of viral nucleotide acid for detecting human herpesviruses and enteroviruses. [PDF]

PLoS ONE, 2015
In this study, a viral microarray based assay was developed to detect the human herpesviruses and enteroviruses associated with central nervous system infections, including herpes simplex virus type 1, type 2 (HSV1 and HSV2), Epstein-Barr virus (EBV ...
Yi Liu, Chunhong Duan, Chunxiu Zhang, Xiaomeng Yang, Yan Zhao, Rui Dong, Jiajing Zhou, Zhongtao Gai   +7 more
doaj   +1 more source

GHRHR Deficiency Enhances Retinal Ganglion Cell Survival and Visual Functions in Experimental Glaucoma by Inhibiting Ferroptosis

Advanced Science, EarlyView.
Glaucoma, a major cause of blindness, involves retinal ganglion cell (RGC) degeneration. This study shows growth hormone‐releasing hormone receptor (GHRHR) deficiency preserves RGC survival and restores vision, unlike activation which only aids survival.
Yan Tong, Ming Ho Yam, Jiaxin Zhang, Lin Du, Linbin Zhou, Yolanda Wong Ying Yip, Bo Man Ho, Hemlata Bisnauthsing, Jiahui Li, Ivan Kong, Shushu Xu, Changzhen Fu, Karl K.H. So, Joaquim S.L. Vong, Ling‐Ping Cen, Ming‐Ming Yang, Khazeema Yousaf, Mai Har Sham, Sun On Chan, Poemen P. Chan, Chi Pui Pang, Clement C. Tham, Jing Na He, Jian Li, Wai Kit Chu   +24 more
wiley   +1 more source

qRT-PCR analysis.

, 2023
Randomly selected eight differential genes and calculated relative gene expressions by qRT-PCR using the 2-ΔΔCt method. Bar graphs indicate the fold difference in transcript levels between the sisal and control under Cd stress; * indicates ...
Tao Chen (75911), Shibei Tan (7470635), Kexian Yi (652533), Jing Li (10611), Weihuai Wu (6896720), Lifang Ge (13907871), Helong Chen (652531), Jingen Xi (652538), Xing Huang (129439), Zhaoxue Huang (17479295), Ping Liu (156158)   +10 more
core   +1 more source

Evaluating an MRNA based body fluid identification test using sybr green fluorescent dye and real-time PCR [PDF]

, 2011
The requirement to have more definitive and wider ranging body fluid identification (BFI) tests has resulted in a range of mRNA based real-time PCR BFI assays utilising Taqman fluorescent dye.
Connolly, Jo-Ann, Williams, Graham
core   +2 more sources