Results 71 to 80 of about 877,269 (241)

qRT-PCR and sequencing.

, 2016
qRT-PCR validation of differentially expressed genes in disease and normal samples of pulmonary artery for 12 genes.
Huabin Cao (2824973), Caiying Zhang (2824976), Fei Yang (46361), Yu Zhuang (2824982), Xiaoquan Guo (2824988), Yalu Song (2824991), Tiancheng Wang (2406016), Ping Liu (156158), Huayuan Lin (2824985), Guoliang Hu (2824979), Qingyang Xiao (686993)   +10 more
core   +1 more source

Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance

BMC Infectious Diseases, 2018
Background During the fifth wave of human H7N9 infections, a novel highly pathogenic (HP) H7N9 variant emerged with an insertion of multiple basic amino acids in the HA cleavage site.
Yang Yang, Shanqin Li, Gary Wong, Sufang Ma, Zhixiang Xu, Xiaonan Zhao, Hong Li, Wen Xu, Haixia Zheng, Jingyan Lin, Qi Zhao, Wenjun Liu, Yingxia Liu, George F. Gao, Yuhai Bi   +14 more
doaj   +1 more source

Targeting the NR1D1–IGF2BP2–V‐ATPase Axis With Hybrid Nanovesicles Restores Macrophage Rhythms to Reverse Sepsis‐Induced Immunosuppression

Advanced Science, EarlyView.
Sepsis disrupts immune‐cell rhythms and weakens bacterial clearance. Biomimetic nanovesicles combining erythrocyte and inflammation‐activated macrophage membranes deliver siNR1D1 to dysfunctional macrophages, restoring the NR1D1–IGF2BP2–V‐ATPase pathway, circadian regulation, phagolysosomal acidification, and antimicrobial defense.
Lang Chen, Wenyi Lin, Lang Jiang, Xuehui Gao, Chenyan Yu, Jili Han, Zhenping Liu, Xuefeng Li, Mei He, Chang Li, Qilan Li, Chaoying Song, Jiqian Xu, You Shang   +13 more
wiley   +1 more source

qRT-PCR primers and associated genes.

, 2016
qRT-PCR primers and associated genes.
Michael A. Cotten (2634094), David L. Denlinger (555367), David S. Kang (2634097), Cheolho Sim (79085)   +3 more
core   +1 more source

Rapid typing diagnosis and clinical analysis of subtypes A and B of human respiratory syncytial virus in children

Virology Journal, 2022
Background Human respiratory syncytial virus (HRSV) is the leading pathogens causing acute respiratory infections (ARI) in children under five years old. We aimed to investigate the distribution of HRSV subtypes and explore the relationship between viral
Zheng Shen, Yuanyuan Zhang, Huamei Li, Lizhong Du   +3 more
doaj   +1 more source

THSD7A Exacerbates Atherosclerosis via Activation of Signaling Axis αvβ3/CEBPD/IL1A

Advanced Science, EarlyView.
THSD7A exerts pro‐inflammatory effects and exacerbates atherosclerosis. Mechanistically, THSD7A regulates endothelial cell inflammation and atherosclerosis by activating the αvβ3/CEBPD/IL1A signaling axis. THSD7A is not only a genetic marker but also a potential therapeutic target for coronary artery disease (CAD).
Jiankun Liu, Hongfei Liu, Yiqi Wan, Andong Wu, Jiayu Qiu, Xueting Gong, Ya Zhao, Yuanyuan Li, Bingbing Zhou, Yangyi Zheng, Fang Wu, Yuanzheng Zhu, Weixin Lv, Xueer Li, Qiang Yuan, Xiao‐Li Tian   +15 more
wiley   +1 more source

qRT-PCR validation of RNA-seq data.

, 2021
Correlation of fold changes determined by RNA-Seq and qRT-PCR data (A). Relative expression levels of ALS1 (B) and ALS2 (C).
Yaling Geng (11356084), Wenliang Pan (319428), Guangyuan Ma (5624378), Jian Li (41607), Libing Yuan (11356081), Hua Wang (13530), Zhiying Hun (11356087), Shanshan Song (500562), Xiaomin Liu (436906)   +8 more
core   +1 more source

Chemotherapy‐Activated GSK3β‐DNMT1 Signaling Upregulates CD47 to Evade Macrophage Phagocytosis and Drive Temozolomide Resistance in Glioblastoma

Advanced Science, EarlyView.
Temozolomide treatment activates GSK3β, driving DNMT1 phosphorylation, destabilization, and CD47 promoter hypomethylation in glioblastoma. This epigenetic shift upregulates CD47, enabling TMZ‐treated GBM cells to evade macrophage phagocytosis, survive chemotherapy, and acquire resistance.
Jie Li, Shuai Ying, Yi Zhang, Jingjing Wang, Quan Wan, Lingli Gong, Daxing Xu, Zhenkun Yang, Hongnian Lu, Ying Yin, Xiangming Fang, Zhening Pu   +11 more
wiley   +1 more source

qRT-PCR validation of RNA-seq results.

, 2016
qRT-PCR validation of RNA-seq results.
Li Kang (494757), Pengfei Wang (123133), Liyuan Wang (795570), Shijin Jiang (3172107), Gen Liu (527432), Ping Zhang (86495), Yunliang Jiang (494758), Yi Sun (118759), Yanping Li (90163), Hao Liu (12832)   +9 more
core   +1 more source

qRT-PCR using standard curves.

, 2021
(A) qRT-PCR standard curves for the individual standard RNAs. The molar amount of the respective RNA fragment introduced into the qRT-PCR reaction is given on the x-axis in logarithmic scale.
Nadine Biedenkopf (2834705), Lennart Kämper (11608114), Stephan Becker (146493), Roland K. Hartmann (9320167), Marcus Lechner (618023), Paul Klemm (6262502), Jana-Christin Demper (9339743), Julia Schlereth (2834699), Simone Bach (9339740), Friedemann Weber (45518), Andreas Schoen (11608111)   +10 more
core   +1 more source