Results 91 to 100 of about 877,269 (241)

Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples

open access: yesInternational Journal of Microbiology
PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze.
Camilo Castellar-Mendoza   +16 more
doaj   +1 more source

SOX30 Facilitates Triple‐Negative Breast Cancer Metastasis via TNFR2–NF‐κB Signaling and Tumor Microenvironment Remodeling

open access: yesAdvanced Science, EarlyView.
SOX30 drives triple‐negative breast cancer (TNBC) metastasis through a dual mechanism: direct activation of the TNFR2/NF‐κB signaling cascade and subsequent CCL20‐mediated recruitment of tumor‐associated macrophages (TAMs) into the tumor microenvironment.
Pingping Gao   +10 more
wiley   +1 more source

qRT-PCR, antigen ELISA, and serology results, Lagos, Nigeria.

open access: yes, 2018
qRT-PCR, antigen ELISA, and serology results, Lagos, Nigeria.
Onikepe Folarin (305194)   +12 more
core   +1 more source

Upadacitinib Restrains the Pathogenic Fitness of CD4+ T Cells and Aberrant B Cell Programming in Optic Neuritis

open access: yesAdvanced Science, EarlyView.
Single‐cell profiling and functional perturbation reveal coordinated JAK1‐pSTAT3 downstream programs in optic neuritis, including MCL1‐dependent fitness of pathogenic CD4+ Tem cells and glycolysis‐linked, cholesterol‐sensitive B‐cell responses associated with RORA. Upadacitinib disrupts this reciprocal T‐B‐cell circuit and alleviates neuroinflammation,
Gengchen Jiang   +12 more
wiley   +1 more source

Quantitative Multiplex Real-Time Reverse Transcriptase–Polymerase Chain Reaction with Fluorescent Probe Detection of Killer Immunoglobulin-Like Receptors, KIR2DL4/3DL3

open access: yesDiagnostics, 2020
(1) Background: KIR2DL4/KIR3DL3 are the framework genes present in all KIR haplotypes, with unique expression patterns being present only in women and CD56bright NK cells. KIR genes have a high degree of DNA sequence identity.
Wipaporn Wongfieng   +3 more
doaj   +1 more source

Multi‐Tissue Crosstalk Regulating Meniscus Healing Under Inflammation and Physiological Loading

open access: yesAdvanced Science, EarlyView.
Using a novel Meniscus‐on‐a‐chip (MoC) model integrated with a meniscus‐specific bioreactor, this study investigated the roles of multi‐tissue crosstalk that regulates injury, healing, and degeneration of the meniscus injury under inflammation and pathophysiological loading.
Hun Jin Jeong   +5 more
wiley   +1 more source

qRT-PCR confirmation of microarray-based transcriptomic results.

open access: yes, 2016
qRT-PCR confirmation of microarray-based transcriptomic results.
Mark J. Longwell (191416)   +12 more
core   +1 more source

qRT-PCR validation of the RNA-Seq data.

open access: yes, 2020
The qRT-PCR data are shown as the means ± standard error (SE) of three replicates. FPKM values from the RNA-Seq analysis are shown as the means and SE of three replicates.
Jinquan Li (112721)   +4 more
core   +1 more source

Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus [PDF]

open access: yes, 2020
Avian flu is a serious disease that always give rise to devastated consequences in poultry industry. This disease is caused by influenza A virus bird’s strain.
Yee, May Ye
core   +1 more source

Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens

open access: yesFrontiers in Microbiology, 2015
A large number of viral and bacterial organisms are responsible for community-acquired pneumonia (CAP) which contributes to substantial burden on health management.
Xuejun eMa
doaj   +1 more source

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