Results 1 to 10 of about 114,774 (119)

An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens

Viruses, 2009
This study used real-time PCR assays to screen small sample volumes for a comprehensive range of 35 respiratory pathogens. Initial thermocycling was limited to 20 cycles to avoid competition for reagents, followed by a secondary real-time multiplex PCR ...
Glenys R. Chidlow, Gerry B. Harnett, Geoffrey R. Shellam, David W. Smith   +3 more
doaj   +3 more sources

Development of a multiplex real-time PCR method for the detection of Pseudomonas savastanoi pv. glycinea and Curtobacterium flaccumfaciens pv. flaccumfaciens in soybean seeds [PDF]

Brazilian Journal of Biology, 2023
Multiplex real-time PCR with TaqMan® probes has been developed for the simultaneous detection of soybean pathogens Pseudomonas savastanoi pv. glycinea and Curtobacterium flaccumfaciens pv. flaccumfaciens.
R. Tarakanov, A. Ignatov, P. Evseev, S. Chebanenko, I. Ignatyeva, K. Miroshnikov, F. Dzhalilov   +6 more
doaj   +1 more source

Development of multiplex real-time PCR assay for the detection of Brucella spp., Leptospira spp. and Campylobacter foetus [PDF]

Veterinaria Italiana, 2014
Abortion among dairy cattle is one of the major causes of economic losses in the livestock industry. This study describes a 1-step multiplex real-time polymerase chain reaction (PCR) to detect Brucella spp., Leptospira spp.
Abdelfattah M. Selim, Mahmoud M. Elhaig, Wolfgang Gaede   +2 more
doaj   +1 more source

Two Multiplex Real-Time PCR Assays to Detect and Differentiate Acinetobacter baumannii and Non- baumannii Acinetobacter spp. Carrying blaNDM, blaOXA-23-Like, blaOXA-40-Like, blaOXA-51-Like, and blaOXA-58-Like Genes. [PDF]

PLoS ONE, 2016
Nosocomial infections caused by Acinetobacter spp. resistant to carbapenems are increasingly reported worldwide. Carbapenem-resistant Acinetobacter (CRA) is becoming a serious concern with increasing patient morbidity, mortality, and lengths of hospital ...
Qiu Yang, Yongyu Rui
doaj   +1 more source

Interpretation of results obtained using real-time fluorescence PCR based on multiplex PCR fragment analysis for ABCB1 tri-allele

Sichuan jingshen weisheng, 2022
ObjectiveTo resolve the problems related to the abnormal interpretations of real-time fluorescence polymerase chain reaction （PCR） results for tri-allele， to formulate the interpretation methods of real-time fluorescence PCR by referring to multiplex PCR
Zhang Yan, Meng Zhuocheng, Xing Huan, Cui Xiaohua, Yang Liu, Zheng Xuan, Dai Zunxiao   +6 more
doaj   +1 more source

Real-time multiplex PCR for human echinococcosis and differential diagnosis

Parasite, 2023
Molecular identification of rare human infectious pathogens appears to be one of the most relevant current methods for rapid diagnosis and management of patients. PCR techniques, in particular real-time quantitative PCR, are best suited for the detection
Knapp Jenny, Lallemand Séverine, Monnien Franck, Felix Sophie, Courquet Sandra, Umhang Gérald, Millon Laurence   +6 more
doaj   +1 more source

Multiplex Real-Time PCR Assay for Six Major Carbapenemase Genes

Pathogens, 2021
The global dissemination of carbapenemase-producing Enterobacteriaceae (CPE) is a major concern in public health. Due to the existence of the diversity of carbapenemases, development of an easily available, cost-effective multiplex detection assay for ...
Nori Yoshioka, Hideharu Hagiya, Matsuo Deguchi, Shigeto Hamaguchi, Masanori Kagita, Isao Nishi, Yukihiro Akeda, Kazunori Tomono   +7 more
doaj   +1 more source

Evaluating performance of multiplex real time PCR for the diagnosis of malaria at elimination targeted low transmission settings of Ethiopia

Malaria Journal, 2022
Background Malaria incidence has declined in Ethiopia in the past 10 years. Current malaria diagnostic tests, including light microscopy and rapid antigen-detecting diagnostic tests (RDTs) cannot reliably detect low-density infections. Studies have shown
Mahlet Belachew, Mistire Wolde, Desalegn Nega, Bokretsion Gidey, Legessie Negash, Ashenafi Assefa, Geremew Tasew, Adugna Woyessa, Adugna Abera   +8 more
doaj   +1 more source

Rapid Detection and Differentiating of the Predominant Salmonella Serovars in Chicken Farm by TaqMan Multiplex Real-Time PCR Assay

Frontiers in Cellular and Infection Microbiology, 2021
Salmonella has been known as an important zoonotic pathogen that can cause a variety of diseases in both animals and humans. Poultry are the main reservoir for the Salmonella serovars Salmonella Pullorum (S.
Suhua Xin, Hong Zhu, Chenglin Tao, Beibei Zhang, Lan Yao, Yaodong Zhang, Dossêh Jean Apôtre Afayibo, Tao Li, Mingxing Tian, Jingjing Qi, Chan Ding, Shengqing Yu, Shaohui Wang   +12 more
doaj   +1 more source

Evaluation of VERSANT® CT/GC DNA 1.0 Assay in conjunction with VERSANT® kPCR Molecular system

Microbiologia Medica, 2011
A total of 463 first void urine specimens were obtained from patients attending the Sexually Transmitted Diseases (STD) Outpatients Clinic of the St. Orsola University Hospital in Bologna, Italy. Samples were tested by the new test VERSANT® CT/GC DNA 1.0
Antonella Marangoni, Alessandra Moroni, Antonietta D’Antuono, Nicoletta Banzola, Enzo Della Bella, Claudio Foschi, Paola Nardini, Paola Monari, Manuela Donati, Roberto Cevenini   +9 more
doaj   +1 more source