Results 41 to 50 of about 18,173,972 (253)

Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize [PDF]

, 2012
Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a
Atoui, Ali, Kallassy, Mireille, El Khoury, André, Lebrihi, Ahmed   +3 more
core   +1 more source

Unique circulating miRNAome signatures captured with distinct molecular states in Lynch syndrome and sporadic colorectal cancer

Molecular Oncology, EarlyView.
Circulating microRNA profiles distinguish nonmalignant Lynch syndrome from cancer‐associated Lynch syndrome, sporadic colorectal cancer, and healthy controls. Age‐stratified analysis reveals disease‐specific regulatory states, and a six‐miRNA panel shows concordant separation in an independent colorectal tissue cohort.
Ramadhani Salum Chambuso, Md Shohel Ul Islam, Rosa M Xicola, Raj Ramesar, Xavier Llor, Sinclair Steele   +5 more
wiley   +1 more source

Multiplex real-time PCR in non-invasive respiratory samples to reduce antibiotic use in community-acquired pneumonia: a randomised trial

Nature Communications
We assessed whether multiplex real-time PCR plus conventional microbiological testing is safe and more effective than conventional microbiological testing alone for reducing antibiotic use in community-acquired pneumonia (CAP).
Gabriela Abelenda-Alonso, Laura Calatayud, Alexander Rombauts, Yolanda Meije, Isabel Oriol, Nieves Sopena, Ariadna Padullés, Jordi Niubó, Alejandra Duarte, Jaume Llaberia, Judit Aranda, Carlota Gudiol, Pau Satorra, Cristian Tebé, Carmen Ardanuy, Jordi Carratalà   +15 more
doaj   +1 more source

Development of a multiplex DNA-based traceability tool for crop plant materials [PDF]

, 2011
The authenticity of food is of increasing importance for producers, retailers and consumers. All groups benefit from the correct labelling of the contents of food products.
Theo W. Prins, Jeroen P. van Dijk, van Dijk, J.P., van Hoef, A.M.A., Kok, E.J., Ralf Seyfarth, Marleen M. Voorhuijzen, Seyfarth, R., A. M. Angeline Van Hoef, Hoef, A.M.A., van, Dijk, J.P., van, Voorhuijzen, M.M., Prins, T.W., Esther J. Kok   +13 more
core   +1 more source

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma

Molecular Oncology, EarlyView.
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara, Kosuke Murakami, Shiro Takamatsu, Akiko Kanto, Seiichi Mori, Tomoyuki Otani, Kazuko Sakai, Yasushi Kotani, Kazuto Nishio, Kaoru Abiko, Noriomi Matsumura   +10 more
wiley   +1 more source

In-house standardization and validation of a multiplex RT-PCR assay for the detection of 13 respiratory viruses

NOVA, 2016
Background.  Multiplex real time PCR is increasingly used to diagnose respiratory viruses and has shown to be superior to traditional methods, such as culture and antigen detection. Objective.
Hernán Vargas, Ángela Diaz, Yamile Celis, Liliana Díaz, Sandra Gómez, Jenny Sánchez, Carlos Golijow, Patricia Arce   +7 more
doaj   +1 more source

Prevalence of bloodstream pathogens is higher in neonatal encephalopathy cases vs. controls using a novel panel of real-time PCR assays. [PDF]

, 2014
BACKGROUND: In neonatal encephalopathy (NE), infectious co-morbidity is difficult to diagnose accurately, but may increase the vulnerability of the developing brain to hypoxia-ischemia.
Klein Nigel, Hughes Peter, Willey Barbara A., Nicola J Robertson, Kurinczuk, JJ, Kurinczuk, Jennifer, Barbara A Willey, Alison M Elliott, Tann Cally J., Were Jackson, Were, J, Elliott, Alison M, Tann, CJ, Robertson, NJ, Peter Hughes, Robertson Nicola J., Jennifer J Kurinczuk, Nkurunziza, Peter, Margaret Nakakeeto, Oweka, James, Klein, Nigel, Were, Jackson, Tann, Cally J, Willey, Barbara A, Hughes, Peter, Robertson, Nicola J, Harris, KA, Nakakeeto, M, Natasha Nyombi, Kurinczuk, Jennifer J, Nakakeeto Margaret, Elliott Alison M., Cally J Tann, Harris, Kathryn A, Nkurunziza Peter, Nyombi, Natasha, Oweka James, Elliott, AM, Nigel Klein, Harris Kathryn A., Kathryn A Harris, Nkurunziza, P, Willey, BA, Nyombi Natasha, Hughes, P, Oweka, J, Jackson Were, Nakakeeto, Margaret, James Oweka, Nyombi, N, Klein, N, Kurinczuk Jennifer J., Peter Nkurunziza   +52 more
core   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Evaluation of the SpeeDx Carba (beta) multiplex real-time PCR assay for detection of NDM, KPC, OXA-48-like, IMP-4-like and VIM carbapenemase genes

BMC Infectious Diseases, 2019
Background Carbapenemase-producing organisms (CPOs) have emerged as antibiotic-resistant bacteria of global concern. Here we assessed the performance of the Carba (beta) assay, a multiplex real-time PCR assay developed by SpeeDx for the detection of key ...
Amanda Bordin, Ella Trembizki, Madeline Windsor, Rachel Wee, Lit Yeen Tan, Cameron Buckley, Melanie Syrmis, Haakon Bergh, Kyra Cottrell, Hosam M. Zowawi, Hanna E. Sidjabat, Patrick N. A. Harris, Graeme R. Nimmo, David L. Paterson, David M. Whiley   +14 more
doaj   +1 more source

Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants.

PLoS ONE, 2020
A multiplex real-time PCR method based on fluorescent TaqMan® probes was developed for the simultaneous detection of the tomato pathogenic bacteria Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and bacterial spot-causing
Eliška Peňázová, Miloň Dvořák, Lucia Ragasová, Tomáš Kiss, Jakub Pečenka, Jana Čechová, Aleš Eichmeier   +6 more
doaj   +1 more source