Results 61 to 70 of about 18,173,972 (253)

Application of real-time and multiplex PCR assays to study leaf blotch epidemics in barley [PDF]

, 2007
Leaf blotch, caused by Rhynchosporium secalis, was studied in a range of winter barley cultivars using a combination of traditional plant pathological techniques and newly developed multiplex and real-time polymerase chain reaction (PCR) assays.
Fountaine, J. M., Shaw, M. W., Fraaije, B. A., Ward, E., Napier, B., Fraaije, B.A., Fountaine, J.M., Shaw, M.W.   +7 more
core   +1 more source

Electropolymerized Aptasensor for Femtogram‐Level Cytokine Detection

Advanced Materials, EarlyView.
Electropolymerization of 3‐amino‐ʟ‐tyrosine creates ultrathin, smooth, and chemically robust pALT nanofilms with accessible carboxyl functionalities for direct aptamer immobilization. Integrated with fiber‐optic SPR and OECT platforms, these interfaces enable IL‐6 detection down to 100 fg mL−1 and provide a versatile route to bioelectronic sensing ...
Jiyao Yu, Renan Colucci, Rachel X. Shi, Qi Lu, Tommaso Marchesi D'Alvise, Ulrike Kraft, Christopher V. Synatschke, Tanja Weil   +7 more
wiley   +1 more source

Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting

Revista da Sociedade Brasileira de Medicina Tropical, 2020
INTRODUCTION: Nontuberculous mycobacteria (NTM) species, as human pathogens, are increasing in the world, as is the difficulty of accurately identifying them. Differential diagnosis, especially between the M.
Aline dos Santos Peixoto, Lílian Maria Lapa Montenegro, Andrea Santos Lima, Fábio Lopes Melo, Walter Lins Barbosa Júnior, Maria Madileuza Carneiro Neves, Jesus Pais Ramos, Haiana Charifker Schindler, Zulma Maria Medeiros   +8 more
doaj   +1 more source

Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction [PDF]

Asian-Australasian Journal of Animal Sciences, 2014
Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gender. However, assessment of the sex ratio of the sorted
Trisadee Khamlor, Petai Pongpiachan, Siwat Sangsritavong, Nipa Chokesajjawatee   +3 more
doaj   +1 more source

CD9+ B Cells Induce T Follicular Helper Cell Apoptosis to Regulate Germinal Center Regression

Advanced Science, EarlyView.
A unique subset of CD9+ B cells regulates germinal center B (GC‐B) cell regression by inducing T follicular helper (Tfh) cell apoptosis through multiple signaling pathways, such as ALCAM‐CD6. FOXP1 plays a key role in GC‐B cell differentiation. These findings clarify mechanisms of GC degeneration and termination and offer potential targets for adenoid ...
Wenjing Liao, Lijuan Song, Meiqian Xu, Tianhao Liang, Yixuan Chen, Gui Chen, Zaihuan Lin, Huakang Du, Guofei Feng, Zhiming Feng, Qi Zhang, Fan Pan, Saixuan Yang, Xinyin Zhang, Wenxu Peng, Tianlong Li, Zhuoshen Lin, Yabang Chen, Jiayi Liu, Baoxin Peng, Yan Liu, Xing Niu, Ting Li, Chengyu Huang, Ke Mo, Jianlei Xie, Yu Zou, Xiaowen Zhang   +27 more
wiley   +1 more source

Establishment and application of a TaqMan-based multiplex real-time PCR for simultaneous detection of three porcine diarrhea viruses

Frontiers in Microbiology
IntroductionPorcine viral diarrhea is a common clinical disease, which results in high mortality and economic losses in the pig industry. Porcine epidemic diarrhea virus (PEDV), porcine rotavirus (PoRV), and porcine deltacoronavirus (PDCoV) are important
Jing Ren, Jing Ren, Congcong Zu, Yang Li, Meng Li, Meng Li, Jinyuan Gu, Jinyuan Gu, Fengling Chen, Fengling Chen, Xiaowen Li, Xiaowen Li   +11 more
doaj   +1 more source

Molecular diagnosis of the human immunodeficiency, Hepatitis B and C viruses among blood donors in Lomé (Togo) by multiplex real time PCR

The Pan African Medical Journal, 2016
This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo.
Maleki Assih, Lochina Feteke, Cyrille Bisseye, Djeneba Ouermi, Florencia Djigma, Simplice Damintoti Karou, Jacques Simpore   +6 more
doaj   +1 more source

Dual Role of Tamoxifen in Enhancing STING and CEACAM1 Expression to Prime a Favorable Tumor Microenvironment for Anti‐TIM3 Immunotherapy

Advanced Science, EarlyView.
Anti‐PD‐1/PD‐L1 blockade has revolutionized cancer immunotherapy, but is ineffective against endocrine‐treated (i.e., Tamoxifen), relapsed ER+ breast cancer (BC) patients. This study provides insight into the sub‐optimal response of ER+BCs to anti‐PD‐1/PD‐L1 blockade – highlighting the induction of STING and the CEACAM1/TIM3 axis after chronic ...
Marvin Angelo E Aberin, Saurabh Singh, Soumya Chatterjee, Guang‐Zhi Sui, Yi‐Fu Wang, Ya‐Ting Lu, Yu‐Ling Lee, Kun‐Yuan Lin, Joy Khag, Ta‐Yu Liu, Shao‐Han Chang, Wai‐Mui Cheung, Hsiao‐Chin Hong, Ren‐Jun Hsu, Chen‐Yang Shen, Chia‐Wei Li, Weng‐Lang Yang, Yao‐Ming Chang, Shih‐Yu Chen, Chandan Guha, Shu‐Ping Wang   +20 more
wiley   +1 more source

Identification of Pathogens in Nasopharyngeal Secretions of Patients with and without Pneumonia by Multiplex RT-PCR Method

Journal of Mazandaran University of Medical Sciences, 2022
Background and purpose: Pneumonia is the most common infectious cause of death. This study aimed at investigating the causative agent of pneumonia in nasopharyngeal secretions by PCR method.
Ahmad Alikhani, Masoud Maboudi, Mohammad Khademloo, Azadeh Khalatbari   +3 more
doaj  

Incremental value of multiplex real-time PCR for the early diagnosis of sepsis in the emergency department

Swiss Medical Weekly, 2014
BACKGROUND: Delayed recognition of sepsis and inappropriate initial antibiotic therapy are associated with increased mortality and morbidity. The early detection of the causative organism in sepsis is an unmet clinical need.
Nora Schaub, Tujana Boldanova, Markus Noveanu, Nisha Arenja, Heinz Hermann, Raphael Twerenbold, Reno Frei, Roland Bingisser, Andrej Trampuz, Christian Müller   +9 more
doaj   +1 more source