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Multiplexed Epitope-Based Tissue Imaging for Discovery and Healthcare Applications [PDF]
Cell Systems, 2016 The study of organs and tissues on a molecular level is necessary as we seek an understanding of health and disease. Over the last few years, powerful highly multiplexed epitope-based imaging approaches that rely on the serial imaging of tissues with ...
Bernd Bodenmiller
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CODEX multiplexed tissue imaging
Nature Reviews Immunology, 2023Garry P. Nolan, Yury Goltsev
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Inpainting missing tissue in multiplexed immunofluorescence imaging
Medical Imaging 2022: Digital and Computational Pathology, 2022Multiplex immunofluorescence (MxIF) is an emerging technique that allows for staining multiple cellular and histological markers to stain simultaneously on a single tissue section. However, with multiple rounds of staining and bleaching, it is inevitable that the scarce tissue may be physically depleted. Thus, a digital way of synthesizing such missing
Shunxing Bao +11 more
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Highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing [PDF]
Nature Biotechnology, 2021 Mapping the localization of multiple proteins in their native three-dimensional (3D) context would be useful across many areas of biomedicine, but multiplexed fluorescence imaging has limited intrinsic multiplexing capability, and most methods for ...
Lixue Shi +2 more
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Highly-Multiplexed Tissue Imaging with Raman Dyes
Journal of Visualized Experiments, 2022Visualizing a vast scope of specific biomarkers in tissues plays a vital role in exploring the intricate organizations of complex biological systems. Hence, highly multiplexed imaging technologies have been increasingly appreciated. Here, we describe an emerging platform of highly-multiplexed vibrational imaging of specific proteins with comparable ...
Lixue, Shi, Mian, Wei, Wei, Min
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Multiplexed Raman Imaging in Tissues and Living Organisms
2021Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) are ideal multiplexing probes for in vivo imaging and tissue staining. Their remarkable sensitivity and unique Raman molecular fingerprint results in minimal background compared to other optical modalities.
Travis M, Shaffer, Sanjiv S, Gambhir
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High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging
2021Multiplexed Ion Beam Imaging by Time of Flight (MIBI-TOF) enables high-dimensional imaging in situ of clinical specimens at single-cell resolution. In MIBI-TOF, tissue sections are stained with dozens of metal-labeled antibodies, whose abundance and location are read by secondary ionization mass spectrometry.
Ofer, Elhanani +2 more
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