Results 41 to 50 of about 3,600,628 (280)

Nontoxic Fluorescent Nanoprobes for Multiplexed Detection and 3D Imaging of Tumor Markers in Breast Cancer

Pharmaceutics, 2023
Multiplexed fluorescent immunohistochemical analysis of breast cancer (BC) markers and high-resolution 3D immunofluorescence imaging of the tumor and its microenvironment not only facilitate making the disease prognosis and selecting effective anticancer
Pavel Sokolov, Galina Nifontova, Pavel Samokhvalov, Alexander Karaulov, Alyona Sukhanova, Igor Nabiev   +5 more
doaj   +1 more source

High-multiplex tissue imaging in routine pathology—are we there yet?

Virchows Archiv, 2023
Abstract High-multiplex tissue imaging (HMTI) approaches comprise several novel immunohistological methods that enable in-depth, spatial single-cell analysis. Over recent years, studies in tumor biology, infectious diseases, and autoimmune conditions have demonstrated the information gain accessible when mapping complex tissues with HMTI.
Jakob Einhaus, Alexander Rochwarger, Sven Mattern, Brice Gaudillière, Christian M. Schürch   +4 more
openaire   +2 more sources

Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology

Nature Communications, 2023
Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex imaging technologies are limited in resolving subcellular biomolecular features.
Yunhao Bai, Bokai Zhu, John-Paul Oliveria, Bryan J. Cannon, Dorien Feyaerts, Marc Bosse, Kausalia Vijayaragavan, Noah F. Greenwald, Darci Phillips, Christian M. Schürch, Samuel M. Naik, Edward A. Ganio, Brice Gaudilliere, Scott J. Rodig, Michael B. Miller, Michael Angelo, Sean C. Bendall, Xavier Rovira-Clavé, Garry P. Nolan, Sizun Jiang   +19 more
doaj   +1 more source

Genetic perturbations go spatial

Cell Genomics, 2022
Tissue-tumor interactivity is the culmination of cell intrinsic features and their extrinsic interactions with the environment. Recently in Cell, Dhainaut and Rose et al.
Katie Teng, Hartland Warren Jackson
doaj   +1 more source

4D Multiplexed Functional Imaging in Deep Tissue [PDF]

Biophysical Journal, 2014
We present a deep tissue multiplexed functional imaging method that probes multiple cellular conditions in live model organisms. The method uses FRET-based biosensors to sense cellular conditions such as calcium and cAMP concentration levels, and uses fluorescence lifetime quantification to interpret the cellular conditions reported by the FRET ...
Zhao, Ming, Wan, Xiaoyang, Zhou, Weibin, Peng, Leilei   +3 more
openaire   +1 more source

Multi-modal image analysis for large-scale cancer tissue studies within IMMUcan

Cell Reports: Methods
Summary: In cancer research, multiplexed imaging allows detailed characterization of the tumor microenvironment (TME) and its link to patient prognosis. The integrated immunoprofiling of large adaptive cancer patient cohorts (IMMUcan) consortium collects
Nils Eling, Julien Dorier, Sylvie Rusakiewicz, Robin Liechti, Preethi Devanand, Michelle Daniel, Jonas Windhager, Bruno Palau Fernandez, Sophie Déglise, Lucie Despland, Abdelkader Benyagoub, Marcin Możejko, Dawid Uchal, Ewa Szczurek, Alexander Loboda, Daaf Sandkuijl, Nikesh Parsotam, Henoch S. Hong, Marie Morfouace, Nicolas Guex, George Coukos, Bernd Bodenmiller, Stephanie Tissot, Daniel Schulz   +23 more
doaj   +1 more source

Multiplex Immunofluorescence and the Digital Image Analysis Workflow for Evaluation of the Tumor Immune Environment in Translational Research

Frontiers in Oncology, 2022
A robust understanding of the tumor immune environment has important implications for cancer diagnosis, prognosis, research, and immunotherapy. Traditionally, immunohistochemistry (IHC) has been regarded as the standard method for detecting proteins in ...
Frank Rojas, Sharia Hernandez, Rossana Lazcano, Caddie Laberiano-Fernandez, Edwin Roger Parra   +4 more
doaj   +1 more source

Aptamer Probes Labeled with Lanthanide‐Doped Carbon Nanodots Permit Dual‐Modal Fluorescence and Mass Cytometric Imaging

Advanced Science, 2021
High‐dimensional imaging mass cytometry (IMC) enables simultaneous quantification of over 35 biomarkers on one tissue section. However, its limited resolution and ultralow acquisition speed remain major issues for general clinical application. Meanwhile,
Youyi Yu, Xin Wang, Xiaolong Jia, Zijian Feng, Lulu Zhang, Hongxia Li, Jie He, Guangxia Shen, Xianting Ding   +8 more
doaj   +1 more source

Efficient Tissue Clearing and Multi-Organ Volumetric Imaging Enable Quantitative Visualization of Sparse Immune Cell Populations During Inflammation

Frontiers in Immunology, 2021
Spatial information of cells in their tissue microenvironment is necessary to understand the complexity of pathophysiological processes. Volumetric imaging of cleared organs provides this information; however, current protocols are often elaborate ...
Julian Hofmann, Julian Hofmann, Iana Gadjalova, Iana Gadjalova, Ritu Mishra, Ritu Mishra, Jürgen Ruland, Jürgen Ruland, Selina J. Keppler, Selina J. Keppler   +9 more
doaj   +1 more source

Dataset: CODEX highly multiplexed tissue imaging in pancreas

, 2023
Human pancreas This dataset was acquired using CODEX, multiplexed single-cell imaging technology for spatial profiling, where all image data is in .tif format and it includes an associated imaging metadata .csv file.
Björklund, Frida, Lundberg, Emma, Martinez Casals, Anna, Patrick E., MacDonald   +3 more
core   +1 more source