Results 51 to 60 of about 3,600,628 (280)
Automatic single cell segmentation on highly multiplexed tissue images [PDF]
Cytometry Part A, 2015 AbstractThe combination of mass cytometry and immunohistochemistry (IHC) enables new histopathological imaging methods in which dozens of proteins and protein modifications can be visualized simultaneously in a single tissue section. The power of multiplexing combined with spatial information and quantification was recently illustrated on breast cancer
Schüffler Peter J +5 more
openaire +3 more sources
Advanced Science, 2023 Here, the authors report that co‐crystallization of fluorophores with matrix‐assisted laser desorption/ionization (MALDI) imaging matrices significantly enhances fluorophore brightness up to 79‐fold, enabling the amplification of innate tissue ...
Ethan Yang +12 more
doaj +1 more source
, 2016 Two-color widefield fluorescence microendoscopy enables multiplexed molecular imaging in the alveolar space of human lung tissue.See readme.txt ...
Choudhary, Tushar R. +6 more
core +2 more sources
Three-dimensional, tomographic super-resolution fluorescence imaging of serially sectioned thick samples. [PDF]
PLoS ONE, 2012 Three-dimensional fluorescence imaging of thick tissue samples with near-molecular resolution remains a fundamental challenge in the life sciences. To tackle this, we developed tomoSTORM, an approach combining single-molecule localization-based super ...
Siddharth Nanguneri +4 more
doaj +1 more source
Combined protein and nucleic acid staining in tissues with PANINI
STAR Protocols, 2022 Summary: We present here a detailed protocol for PANINI (protein and nucleic acid in situ imaging), a technique that enables the concurrent staining of protein and nucleic acids in archival tissue sections.
Addison Deisher, Yao Yu Yeo, Sizun Jiang
doaj +1 more source
, 2022 Multiplexed imaging technologies enable the study of biological tissues at single-cell resolution while preserving spatial information. Currently, high-dimension imaging data analysis is technology-specific and requires multiple tools, restricting ...
Lucia Montorsi (11471518) +10 more
core +2 more sources
Multiplexed imaging reveals an IFN-γ-driven inflammatory state in nivolumab-associated gastritis
Cell Reports Medicine, 2021 Summary: Immune checkpoint blockade using PD-1 inhibition is an effective approach for treating a wide variety of cancer subtypes. While lower gastrointestinal (GI) side effects are more common, upper gastrointestinal adverse events are rarely reported ...
Selena Ferrian +10 more
doaj +1 more source
Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy
FEBS Letters, EarlyView.The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley +1 more source
Multiplexed 3D FRET imaging in deep tissue of live embryos [PDF]
Scientific Reports, 2015 AbstractCurrent deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) sensors in live embryos ...
Ming Zhao +4 more
openaire +2 more sources
MIBI dataset for Expanded Vacuum-Stable Gels for Multiplexed High Resolution Spatial Histopathology
, 2023 Multiplexed MIBI images used in Figures for manuscript "Expanded Vacuum-Stable Gels for Multiplexed High Resolution Spatial Histopathology", a manuscript described a method permitting the accomendation of expansion microscopy (ExM) hydrogel in high ...
Zhu, Bokai +19 more
core +1 more source