Results 61 to 70 of about 40,419 (211)

Quantification of High-Molecular Weight Protein Platforms by AQUA Mass Spectrometry as Exemplified for the CD95 Death-Inducing Signaling Complex (DISC)

Cells, 2013
Contemporary quantitative mass spectrometry provides fascinating opportunities in defining the stoichiometry of high-molecular weight complexes or multiprotein platforms.
Inna Lavrik, Uwe Warnken, Kolja Schleich, Martina Schnölzer   +3 more
doaj   +1 more source

Organization of multiprotein complexes at cell–cell junctions [PDF]

Histochemistry and Cell Biology, 2008
The formation of stable cell-cell contacts is required for the generation of barrier-forming sheets of epithelial and endothelial cells. During various physiological processes like tissue development, wound healing or tumorigenesis, cellular junctions are reorganized to allow the release or the incorporation of individual cells.
openaire   +2 more sources

The E3 Ligase RNF115 Aggravates Pathological Cardiac Hypertrophy via Ubiquitin‐Mediated Degradation of SPTBN1

Advanced Science, EarlyView.
In response to hypertrophic stimuli, increased c‑JUN phosphorylation upregulates RNF115, leading to SPTBN1 ubiquitination and degradation. which promotes F‑actin depolymerization and YAP activation, driving cardiac hypertrophy. The RNF115 inhibitor DTD effectively suppresses SPTBN1 ubiquitination and cardiac hypertrophy.
Yan Zu, Shiqing Chen, Ji Chen, Liuliu Ruan, Bowen Li, Weijian Chu, Shichen Huang, Dian Yu, Jing Tian, Yuhan Zhao, Yi Han, Xin Tang, Yong Ji   +12 more
wiley   +1 more source

Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes

Nature Communications
Most cellular processes are carried out by multiprotein assemblies. Although various molecular tools exist to visualize binary protein interactions in live cells, the visualization of multiprotein complexes remains a challenge.
Sara Bottone, Fanny Broch, Antoine Gedeon, Aurélien Brion, Lina El Hajji, Hela Benaissa, Arnaud Gautier   +6 more
doaj   +1 more source

Targeted tandem affinity purification of PSD‐95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins

Molecular Systems Biology, 2009
The molecular complexity of mammalian proteomes demands new methods for mapping the organization of multiprotein complexes. Here, we combine mouse genetics and proteomics to characterize synapse protein complexes and interaction networks.
Esperanza Fernández, Mark O Collins, Rachel T Uren, Maksym V Kopanitsa, Noboru H Komiyama, Mike D R Croning, Lysimachos Zografos, J Douglas Armstrong, Jyoti S Choudhary, Seth G N Grant   +9 more
doaj   +1 more source

GS‐9620 alleviates psoriasis‐like inflammation by regulating autophagy in keratinocytes

Animal Models and Experimental Medicine, EarlyView.
Psoriasis is an immune‐mediated inflammatory disease of the skin characterized by excessive proliferation and abnormal differentiation of keratinocytes. GS‐9620 can increase the expression of ATG5, ATG7 and ATG16L1, thereby regulating the autophagy of keratinocytes and inhibiting the secretion of related inflammatory factors.
Yansi Lyu, Wei Zhou, Pei Zhang, Chen Lin, Xin Wen, Zigang Zhao, Guoqiang Zhang, Qian Zhang, Si Chen   +8 more
wiley   +1 more source

Cold Gas Plasma Induces Platelet Activation and Hemostasis in Native and Anticoagulated Human Blood

Advanced NanoBiomed Research, EarlyView.
Cold physical gas plasma induces platelet activation and hemostatic responses in native and anticoagulated human blood. Treatment efficacy depends on plasma parameters, including gas composition, distance, and exposure mode, which govern reactive species delivery and energy transfer.
Sander Bekeschus, Broder Poschkamp, Lea Miebach, Anke Schmidt, Linus Hübner, Julia van der Linde   +5 more
wiley   +1 more source

Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis

STAR Protocols, 2020
Summary: By using negatively charged Coomassie brilliant blue G-250 dye to induce a charge shift on proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE) allows resolution of enzymatically active multiprotein complexes extracted from ...
Alba Timón-Gómez, Rafael Pérez-Pérez, Eva Nyvltova, Cristina Ugalde, Flavia Fontanesi, Antoni Barrientos   +5 more
doaj   +1 more source

The integration of signaling by multiprotein complexes containing Raf kinases

, 2007
In vivo, eukaryotic cells are subjected simultaneously to a broad array of signals ranging from mitogens and inflammatory inputs to environmental stresses and developmental cues.
Kyriakis, John M., John M. Kyriakis
core   +1 more source

RBMX Transcriptionally Repressed by EZH2‐Associated H3K27me3 Modification Attenuates Pyroptosis in Renal Ischemia/Reperfusion Injury via Regulating SIRT3/NLRP3 Inflammasome Activation

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Ischemic reperfusion injury (IRI) to kidney is a significant clinical factor in acute kidney injury (AKI). This study aimed to investigate the new role of RNA‐binding motif protein X‐linked (RBMX), a modulator of m6A methylation, in renal IRI and to examine the associated regulatory mechanisms. An in vitro renal IRI model was established using
Yi‐Han Wang, Yan Teng, Fang‐Lan Yao, Shu‐Qi Wei, Yan Li   +4 more
wiley   +1 more source