Results 71 to 80 of about 960,142 (193)

Single-molecule visualization of fast polymerase turnover in the bacterial replisome

eLife, 2017
The Escherichia coli DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high efficiency and fidelity.
Jacob S Lewis, Lisanne M Spenkelink, Slobodan Jergic, Elizabeth A Wood, Enrico Monachino, Nicholas P Horan, Karl E Duderstadt, Michael M Cox, Andrew Robinson, Nicholas E Dixon, Antoine M van Oijen   +10 more
doaj   +1 more source

Phosphorylation-Dependent Assembly of a 14-3-3 Mediated Signaling Complex during Red Blood Cell Invasion by Plasmodium falciparum Merozoites

mBio, 2020
Red blood cell (RBC) invasion by Plasmodium merozoites requires multiple steps that are regulated by signaling pathways. Exposure of P. falciparum merozoites to the physiological signal of low K+, as found in blood plasma, leads to a rise in cytosolic ...
Kunal R. More, Inderjeet Kaur, Quentin Giai Gianetto, Brandon M. Invergo, Thibault Chaze, Ravi Jain, Christéle Huon, Petra Gutenbrunner, Hendrik Weisser, Mariette Matondo, Jyoti S. Choudhary, Gordon Langsley, Shailja Singh, Chetan E. Chitnis   +13 more
doaj   +1 more source

WDR12 Promotes Glucose Metabolism via PFKFB4 Upregulation in Gastric Cancer

Cancer Science, EarlyView.
WDR12 promotes glucose metabolism and tumor growth in GC. Mechanistically, WDR12 interacts with TDP43 and appears to enhance PFKFB4 expression, thereby promoting glucose metabolism. ABSTRACT Metabolic reprogramming is a hallmark of gastric cancer (GC); however, the role of transcriptional regulators in modulating glycolysis remains incompletely ...
Rutong Zhang, Dehua Liu, Xiaoxi Feng, Aoxing Cheng, Zhenye Yang, Kaiguang Zhang   +5 more
wiley   +1 more source

Phosphorylation-mediated interactions with TOPBP1 couple 53BP1 and 9-1-1 to control the G1 DNA damage checkpoint

eLife, 2019
Coordination of the cellular response to DNA damage is organised by multi-domain ‘scaffold’ proteins, including 53BP1 and TOPBP1, which recognise post-translational modifications such as phosphorylation, methylation and ubiquitylation on other proteins ...
Nicolas Bigot, Matthew Day, Robert A Baldock, Felicity Z Watts, Antony W Oliver, Laurence H Pearl   +5 more
doaj   +1 more source

Robots, pipelines, polyproteins:enabling multiprotein expression in prokaryotic and eukaryotic cells

, 2011
Multiprotein complexes catalyze vital biological functions in the cell. A paramount objective of the SPINE2 project was to address the structural molecular biology of these multiprotein complexes, by enlisting and developing enabling technologies for ...
Arnaud Poterszman, Frederic Garzoni, Lakshmi Sumitra Vijayachandran, Poterszman, Arnaud, Romier, Christophe, Christoph Bieniossek, Christophe Romier, Busso, Didier, Timothy J. Richmond, Bieniossek, Christoph, Chaillet, Maxime, Didier Busso, Garzoni, Frederic, Richmond, Timothy J, Schaffitzel, Christiane, Berger, Imre, Richmond, Timothy, Cristina Viola, Simon Trowitzsch, Christiane Schaffitzel, Maxime Chaillet, Trowitzsch, Simon, Viola, Cristina, Imre Berger, Vijayachandran, Lakshmi Sumitra   +24 more
core   +1 more source

Biallelic Truncating Variant in LRGUK Is Associated With Severe Multiple Morphological Abnormalities of the Sperm Flagella and Sperm Nuclear Defects in Humans

Clinical Genetics, EarlyView.
A homozygous truncating variant in LRGUK results in loss of the LRGUK protein and causes multiple morphological abnormalities of the flagella (MMAF), disrupted axonemal architecture, central pair defects, abnormal chromatin organization, and severe male infertility, establishing LRGUK as a novel human infertility gene.
Wiâme Mokkedem, Zeina Wehbe, Anne‐Laure Barbotin, Angèle Boursier, Célia Tebbakh, Nicolas Thierry‐Mieg, Marie Bidart, Antonella Migliaccio, Rosanna Chianese, Christophe Arnoult, Raoudha Zouari, Zine‐Eddine Kherraf, Pierre F. Ray, Guillaume Martinez, Charles Coutton   +14 more
wiley   +1 more source

Equi‐MOI ratio for rapid baculovirus‐mediated multiprotein co‐expression in insect cells integrating selenomethionine for structural studies

FEBS Open Bio
Proteins often co‐exist as multicomponent assemblies, making their co‐expression essential in recombinant production processes. The baculovirus expression vector system is commonly used to produce recombinant multiprotein complexes mostly for structural ...
Andrej Bitala, Mário Benko, Marek Nemčovič, Ivana Nemčovičová   +3 more
doaj   +1 more source

Architecture of the human mTORC2 core complex

eLife, 2018
The mammalian target of rapamycin (mTOR) is a key protein kinase controlling cellular metabolism and growth. It is part of the two structurally and functionally distinct multiprotein complexes mTORC1 and mTORC2.
Edward Stuttfeld, Christopher HS Aylett, Stefan Imseng, Daniel Boehringer, Alain Scaiola, Evelyn Sauer, Michael N Hall, Timm Maier, Nenad Ban   +8 more
doaj   +1 more source

The production of multiprotein complexes in insect cells using the baculovirus expression system

, 2014
The production of a homogeneous protein sample in sufficient quantities is an essential prerequisite not only for structural investigations but represents also a rate-limiting step for many functional studies.
Abdulrahman, Wassim, Olga Kolesnikova, Garzoni, Frederic, Gupta, Kapil, Arnaud Poterszman, Wassim Abdulrahman, Laura Radu, Frederic Garzoni, Berger, Imre, Poterszman, Arnaud, Kolesnikova Karchenkov, Olga, Judit Osz-Papai, Imre Berger, Kapil Gupta, Osz-Papai, Judit, Kolesnikova, Olga, Radu, Laura   +16 more
core   +1 more source

The sorting nexin Snx21 promotes flotillin‐mediated endocytosis

The FEBS Journal, EarlyView.
In this study, we describe a novel function of the previously uncharacterized Snx21 protein as a specific positive regulator of flotillin‐mediated endocytic trafficking. Snx21 is recruited to endosomal membranes via binding to PI(3)P, and it colocalizes with flotillins on the surface of late endosomes.
Tamás Maruzs, Enikő Lakatos, Dalma Feil‐Börcsök, Gábor Juhász, Regina Mező, Asha Kiran Maddali, András Blastyák, Aladár Pettkó‐Szandtner, Hajnalka Laczkó‐Dobos, Gábor Juhász   +9 more
wiley   +1 more source