Results 41 to 50 of about 40,419 (211)
A general path for large-scale solubilization of cellular proteins: From membrane receptors to multiprotein complexes [PDF]
Protein Expression and Purification, 2013 Filippo Pullara +2 more
exaly +2 more sources
DNMT1 is a Component of a Multiprotein DNA Replication Complex [PDF]
Cell Cycle, 2002 DNA methylation is a major determinant of epigenetic inheritance and plays an important role in genome stability. The accurate propagation of DNA methylation patterns with cell division requires that methylation be closely coupled to DNA replication, however the precise molecular determinants of this interaction have not been defined.
Paula M, Vertino +6 more
openaire +2 more sources
Genetic code expansion for multiprotein complex engineering [PDF]
Nature Methods, 2016 We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins ...
Christine Koehler +26 more
openaire +4 more sources
Nuclear Coregulatory Complexes in Tregs as Targets to Promote Anticancer Immune Responses
Frontiers in Immunology, 2022 T-regulatory (Treg) cells display considerable heterogeneity in their responses to various cancers. The functional differences among this cell type are heavily influenced by multiprotein nuclear complexes that control their gene expression.
Lanette M. Christensen +2 more
doaj +1 more source
Purifi cation of Multiprotein Histone Acetyltransferase Complexes [PDF]
, 2011 The reversible acetylation of specific lysine residues on core histones regulates gene transcription in eukaryotes. Since the discovery of GCN5 as the first transcription-regulating histone acetyltransferase (HAT), a variety of HATs have now been identified and shown to acetylate different sites on histones as well as on non-histone proteins, including
Yuan-Liang, Wang +2 more
openaire +2 more sources
SmartBac, a new baculovirus system for large protein complex production
Journal of Structural Biology: X, 2019 Recent revolution of cryo-electron microscopy has opened a new door to solve high-resolution structures of macromolecule complexes without crystallization while how to efficiently obtain homogenous macromolecule complex sample is therefore becoming a ...
Yujia Zhai +4 more
doaj +1 more source
PLoS Pathogens, 2022 The hepatitis C virus (HCV) life cycle is highly regulated and characterized by a step-wise succession of interactions between viral and host cell proteins resulting in the assembly of macromolecular complexes, which catalyse genome replication and/or ...
Olaf Isken +5 more
doaj +1 more source
Membrane-embedded multiprotein complexes coproduced with GFP from the PprgH::gfp fusion.
, 2022 Membrane-embedded multiprotein complexes coproduced with GFP from the PprgH::gfp fusion.
Abram Aertsen (43457) +12 more
core +1 more source
Hypergraphs for predicting essential genes using multiprotein complex data [PDF]
, 2020 Protein–protein interactions are crucial in many biological pathways and facilitate cellular function. Investigating these interactions as a graph of pairwise interactions can help to gain a systemic understanding of cellular processes.
Deane, Charlotte +2 more
core +1 more source
A Multiprotein Complex That Mediates Translational Enhancement in Drosophila [PDF]
Journal of Biological Chemistry, 2007 Modulating the efficiency of translation plays an important role in a wide variety of cellular processes and is often mediated by trans-acting factors that interact with cis-acting sequences within the mRNA. Here we show that a cis-acting element, the Hsp83 degradation element (HDE), within the 3'-untranslated region of the Drosophila Hsp83 mRNA ...
Meryl R, Nelson +7 more
openaire +2 more sources