Acetylcholine-treated murine dendritic cells promote inflammatory lung injury [PDF]
PLoS ONE, 2019 In recent years a non-neuronal cholinergic system has been described in immune cells, which is often usually activated during the course of inflammatory processes. To date, it is known that Acetylcholine (ACh), a neurotransmitter extensively expressed in
Alcain, Julieta María +8 more
core +2 more sources
M3 Muscarinic Acetylcholine Receptor-Mediated Signaling Is Regulated by Distinct Mechanisms
Molecular Pharmacology, 2008 We have used RNA interference previously to demonstrate that G protein-coupled receptor kinase 2 (GRK2) regulates endogenously expressed H1 histamine receptor in human embryonic kidney 293 cells. In this report, we investigate the regulation of endogenously expressed M(3) muscarinic acetylcholine receptor (M(3) mAChR).
Jiansong Luo +2 more
openaire +3 more sources
Signaling Bias and Antagonism of Pilocarpine for M3 Muscarinic Acetylcholine Receptor
The FASEB Journal, 2018 Pilocarpine is a prototypical drug used to treat glaucoma and dry mouth since the 19th century and classified as either a full or partial muscarinic agonist. Here, we report several unexpected results pertaining to its interaction with its main therapeutic target ‐ muscarinic M3 receptor (M3R).
Kelly Wang +2 more
openaire +1 more source
Spatial intensity distribution analysis: studies of G Protein-coupled receptor oligomerization [PDF]
, 2018 Spatial intensity distribution analysis (SpIDA) is a recently developed approach for determining quaternary structure information on fluorophore-labelled proteins of interest in situ. It can be applied to live or fixed cells and native tissue.
Marsango, Sara +3 more
core +1 more source
A novel M3 muscarinic acetylcholine receptor is expressed in chick atrium and ventricle.
Journal of Biological Chemistry, 1994 Prior studies have suggested that heart expresses only the M2 isoform of the muscarinic receptor (Peralta, E.G., Ashkenazi, A., Winslow, J.W., Smith, D.H., Ramachandran, J., and Capon, D.J. (1987) EMBO J. 6, 3923-3929). Tietje and Nathanson (Tietje, K.M., and Nathanson, N. M. (1991) J. Biol. Chem.
Albert P. Gadbut, Jonas B. Galper
openaire +3 more sources
Modulation of c-Jun NH2-Terminal (JNK) by Cholinergic Autoantibodies from Patients with Sjögren’s Syndrome [PDF]
, 2011 Background: We wanted to determine (via an immunopharmacological approach) whether the c-Jun NH2 terminal kinase (JNK) cascade is phosphorylated in the submandibular gland by carbachol and cholinergic autoantibodies (IgG) present in the sera of patients ...
Borda, Enri Santiago +3 more
core +1 more source
Generation of an agonistic binding site for blockers of the M3 muscarinic acetylcholine receptor [PDF]
Biochemical Journal, 2008 GPCRs (G-protein-coupled receptors) exist in a spontaneous equilibrium between active and inactive conformations that are stabilized by agonists and inverse agonists respectively. Because ligand binding of agonists and inverse agonists often occurs in a competitive manner, one can assume an overlap between both binding sites.
Angela Schulz +3 more
openaire +3 more sources
A molecular basis for selective antagonist destabilization of dopamine D3 receptor quaternary organization [PDF]
, 2017 The dopamine D3 receptor (D3R) is a molecular target for both first-generation and several recently-developed antipsychotic agents. Following stable expression of this mEGFP-tagged receptor, Spatial Intensity Distribution Analysis indicated that a ...
Caltabiano, Gianluigi +6 more
core +2 more sources
Presynaptic M1 muscarinic cholinoceptors mediate inhibition of excitatory synaptic transmission in the hippocampus in vitro [PDF]
, 1990 The effects of the cholinoceptor agonist, carbachol (CCh), were examined in the rat hippocampal slice preparation. Intracellular recordings from CA1 pyramidal neurones revealed that CCh (1–3 μM) inhibited excitatory postsynaptic responses evoked by ...
Sheridan, Robert D., Sutor, Bernd
core +1 more source
Muscarinic M2 acetylcholine receptor distribution in the guinea-pig gastrointestinal tract [PDF]
, 2006 In the enteric nervous system, acetylcholine is the most common neurotransmitter to induce gastrointestinal smooth muscle contractions. Cholinergic signaling is mediated by muscarinic acetylcholine receptors on the surface of smooth muscle cells.
58998 +3 more
core +1 more source