Results 211 to 220 of about 5,512,789 (304)

Estimating mutation rate and characterising single nucleotide de novo mutations in pigs. [PDF]

Genet Sel Evol
Rochus CM, Steensma MJ, Bink MCAM, Huisman AE, Harlizius B, Derks MFL, Crooijmans RPMA, Ducro BJ, Bijma P, Groenen MAM, Mulder HA.   +10 more
europepmc   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Parameterizing Pantherinae: De Novo Mutation Rate Estimates from Panthera and Neofelis Pedigrees. [PDF]

Genome Biol Evol
Armstrong EE, Carey SB, Harkess A, Zenato Lazzari G, Solari KA, Maldonado JE, Fleischer RC, Aziz N, Walsh P, Koepfli KP, Eizirik E, Petrov DA, Campana MG.   +12 more
europepmc   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Direct measurement of the male germline mutation rate in individuals using sequential sperm samples. [PDF]

Nat Commun
Shoag JE, Srinivasa A, Loh CA, Liu MH, Lassen E, Melanaphy S, Costa BM, Grońska-Pęski M, Jabara NT, Picciotto S, Choi U, Bohorquez AD, Barbieri CE, Callum P, Skytte AB, Evrony GD.   +15 more
europepmc   +1 more source

One size does not fit all: An in vitro evaluation of the effects of bezafibrate and medroxyprogesterone acetate on human SH‐SY5Y and U‐87 MG cancer cells

FEBS Open Bio, EarlyView.
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar, Francesco Michelangeli, Farhat Latif Khanim, Christopher Martin Bunce, William Eustace Basil Johnson   +4 more
wiley   +1 more source

Direct Measurement of the Mutation Rate and Its Evolutionary Consequences in a Critically Endangered Mollusk. [PDF]

Mol Biol Evol
Wooldridge TB, Ford SM, Conwell HC, Hyde J, Harris K, Shapiro B.   +5 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Continuous evolution of user-defined genes at 1 million times the genomic mutation rate. [PDF]

Science
Rix G, Williams RL, Hu VJ, Spinner A, Pisera AO, Marks DS, Liu CC.   +6 more
europepmc   +1 more source