Results 231 to 240 of about 81,793 (268)

How to isolate cardiac myocytes

Cardiovascular Research, 1994
A complete technique is described for the isolation of myocytes from mammalian hearts using the Langendorff perfusion technique. The use of calcium-free solution containing collagenase and protease, followed by low calcium solution, consistently results in a large number of calcium tolerant myocytes which are well suited for long periods of ...
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Cardiac Myocyte Terminal Differentiation

Annals of the New York Academy of Sciences, 1995
The exact mechanism of terminal differentiation in cardiac myocytes is currently unknown. Studies in the skeletal muscle system provided a model where muscle lineage termination gene directly interacts with Rb to produce and maintain the terminally differentiated state. This interaction provided the critical components for the lock in cell cycle arrest
S K, Tam, W, Gu, V, Mahdavi, B, Nadal-Ginard   +3 more
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Confocal Microscopy of Cardiac Myocytes

2013
Detailed methods are provided for the preparation and confocal imaging of cardiac myocyte development and differentiation. Examples include protocols for the analysis of cultured myocytes as well as vibratome sections of hearts from embryonic and adult tissue.
Robert L, Price, Stephen T, Haley, Tara, Bullard, Jeffrey, Davis, Thomas K, Borg, Louis, Terracio   +5 more
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Scaffolding Proteins in Cardiac Myocytes

2008
Post-translational modification, such as protein phosphorylation, plays a critical role to reversibly amplify and modulate signaling pathways. Since kinases and phosphatases have broad substrate recognition motifs, compartmentalization and localization of signaling complexes are required to achieve specific signals.
N L, Chudasama, S O, Marx, S F, Steinberg   +2 more
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Adrenergic regulation of cardiac myocyte apoptosis

Journal of Cellular Physiology, 2001
AbstractThe direct effects of catecholamines on cardiac myocytes may contribute to both normal physiologic adaptation and pathologic remodeling, and may be associated with cellular hypertrophy, apoptosis, and alterations in contractile function.
K, Singh, L, Xiao, A, Remondino, D B, Sawyer, W S, Colucci   +4 more
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The association of lysophosphatidylcholine with isolated cardiac myocytes

Lipids, 1990
AbstractThe ability of exogenous lysophosphatidylcholine to produce electrophysiological derangements and cardiac arrhythmias in the heart has been documented. The action of lysophosphatidylcholine is thought to be mediated via its association with the membrane.
Man, RYK, Kinnaird, AAA, Bihler, I, Choy, PC   +3 more
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Cardiac myocytes and the cardiac action potential

2010
Cardiac myocytes are the contractile cells of the heart and constitute the bulk of heart mass. There are differences between the myocytes of the ventricles, the atria, and the conduction system: ventricular myocytes are elongated cells and packed with myofibrils (the contractile apparatus) and mitochondria (for ATP production)....
Kenneth T. MacLeod, Steven B. Marston, Philip A. Poole-Wilson, Nicholas J. Severs, Peter H. Sugden   +4 more
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Calcium Signaling in Cardiac Ventricular Myocytes

Annals of the New York Academy of Sciences, 2005
Abstract: Calcium (Ca) is a multifunctional regulator of diverse cellular functions. In cardiac muscle Ca is a direct central mediator of electrical activation, ion channel gating, and excitation‐contraction (E‐C) coupling that all occur on the millisecond time scale. The key amplification step in E‐C coupling is under tight control of very local [Ca].
Donald M, Bers, Tao, Guo
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Regulation of cardiac myocyte cell death

Pharmacology & Therapeutics, 2003
Cardiac myocyte death, whether through necrotic or apoptotic mechanisms, is a contributing factor to many cardiac pathologies. Although necrosis and apoptosis are the widely accepted forms of cell death, they may utilize the same cell death machinery. The environment within the cell probably dictates the final outcome, producing a spectrum of response ...
Angela, Clerk, Sharon M, Cole, Timothy E, Cullingford, Joanne G, Harrison, Mika, Jormakka, Donna M, Valks   +5 more
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Leptofibrils in Cardiac Myocytes

Ultrastructural Pathology, 1988
Eloisa Arbustini, Victor Ferrans, H. David, F. N. Ghadially   +3 more
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