Results 201 to 210 of about 3,735 (212)

The hydrolysis of unsubstituted N-acylhomoserine lactones to their homoserine metabolites

Journal of Chromatography A, 2007
Derivatives of N-acylhomoserine lactones (HSLs) with different alkanoyl side chains occur as quorum or diffusion sensing molecules in gram-negative bacteria and their quantitative chemical analysis became important as a possible way to follow regulation processes of their pathogenicity towards plants and animals.
Christina Kuttler, Xiaojing Li, Matthias Englmann, Agnes Fekete, Philippe Schmitt-Kopplin, Jenoe Fekete, Moritz Frommberger, Istvan Gebefügi   +7 more
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Discovering Additional Chemical and Biological Functions for 3-Oxo-N-Acylhomoserine Lactones [PDF]

, 2010
Introduction The term quorum sensing has been coined to describe the ability of a population of unicellular bacteria to act as a multicellular organism in a cell-density-dependent manner, that is, a way to sense “how many are out there” [1]. Bacteria use small diffusible molecules to exchange information amongst themselves.
Tobin J. Dickerson, Kim D. Janda, Claude J. Rogers, Jason A. Moss, Sang-Hyeup Lee, Rafaella Sartorio, Gunnar F. Kaufmann, Bruce Clapham, Michael M. Meijler, Andrew P. Brogan   +9 more
openaire   +1 more source

Synthesis of Multiple N-Acylhomoserine Lactones is Wide-spread Among the Members of the Burkholderia cepacia Complex

Systematic and Applied Microbiology, 2001
Seventy strains of the Burkholderia cepacia complex, which currently comprises six genomic species, were tested for their ability to produce N-acylhomoserine lactone (AHL) signal molecules. Using thin layer chromatography in conjunction with a range of AHL biosensors, we show that most strains primarily produce two AHLs, namely N-octanoylhomoserine ...
Leo Eberl, Peter Vandamme, Andrea Hardman, Anette Steidle, Paul Williams, Burkhard Tümmler, Kathrin Riedel, Barry Middleton, Astrid Gotschlich, Otto Geisenberger, Andreas Tögl, Birgit Huber, Miguel Cámara, Philip J. Hill   +13 more
openaire   +2 more sources

Distribution and characterization of N-acylhomoserine lactone (AHL)-degrading activity and AHL lactonase gene (qsdS) in Sphingopyxis

Journal of Bioscience and Bioengineering, 2019
N-Acylhomoserine lactone (AHL)-degrading enzyme is identified from the various environments and applied for quorum-sensing inhibition. In this study, we isolated two AHL-degrading strains, Sphingopyxis sp. EG6 and FD7, from the industrial cooling water samples.
Taro Iizumi, Yaoki Kamimura, Niina Sato, Tomohiro Morohoshi   +3 more
openaire   +2 more sources

Facile preparation of deuterium-labeled N-acylhomoserine lactones as internal standards for isotope dilution mass spectrometry

Bioorganic & Medicinal Chemistry, 2010
N-Acylhomoserine lactones (AHLs) are widely conserved signal molecules that mediate quorum sensing in Gram-negative bacteria. In this study, deuterium-labeled AHLs were prepared for use as internal standards for isotope dilution mass spectrometry. Their utility in the sensitive and precise quantification of AHLs in culture supernatants of bacteria by ...
Hideo Hayashi, Kenji Kai, Ayaka Tani
openaire   +3 more sources

The cloning and characterization of one novel metagenome-derived thermostable esterase acting on N-acylhomoserine lactones

Journal of Molecular Catalysis B: Enzymatic, 2012
Abstract A novel gene (designated as est816 ) encoding an esterase was isolated from a Turban Basin metagenomic library with a functional screening method. Sequence analysis revealed that est816 encoded a protein of 271 amino acids with a predicted molecular mass (Mr) of 29.9 kDa and was expressed in Escherichia coli BL21 (DE3) in soluble form ...
Xiaolong Liu, Xinjiong Fan, Yuhuan Liu
openaire   +2 more sources

Cell aggregation is negatively regulated by N-acylhomoserine lactone-mediated quorum sensing in Pantoea ananatis SK-1

Journal of Bioscience and Bioengineering, 2011
Pantoea ananatis SK-1 produces N-acylhomoserine lactones (AHLs) and regulates expression of some virulence factors through AHL-mediated quorum sensing. In this study, we discovered that the strain SK-02I, which has a disrupted AHL-synthetic gene, showed constitutive cell aggregation.
Yuji Ogata, Tomohiro Morohoshi, Tsukasa Ikeda   +2 more
openaire   +3 more sources

Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris

Journal of Bioscience and Bioengineering, 2012
N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signal molecules by many gram-negative bacteria. We have reported that Solibacillus silvestris, which was isolated from the potato leaf, has AHL-degrading activity. To identify the AHL-degrading gene, whole genome sequencing of S.
Yoshiaki Tominaga, Tsukasa Ikeda, Nobutaka Someya, Tomohiro Morohoshi   +3 more
openaire   +3 more sources

6.12 Detection, Purification, and Synthesis of n-acylhomoserine Lactone Quorum Sensing Signal Molecules

1998
Publisher Summary This chapter describes the most relevant practical approaches for the identification and characterization of the N-acylhomoserine lactones (AHL) family of quorum sensing signal molecules. The first indication of AHL-mediated gene regulation in a Gram-negative bacterial pathogen is the appearance of a specific phenotype in a cell ...
Siri Ram Chhabra, Mavis Daykin, Miguel Cámara   +2 more
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Inhibitory control of quorum sensing using N-acylhomoserine lactone lactonase

Journal of Bioscience and Bioengineering, 2009
Norihiro Kato, Tsukasa Ikeda, Takamasa Murai, Hiroshi Motohashi, Azumi Kobayashi, Tomohiro Morohoshi   +5 more
openaire   +2 more sources